Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography

Chen, Xiao‐Jia; Guo, Baoyuan; Li, Shao‐Ping; Zhang, Q.W.; Tu, Peng‐Fei; Wang, Y.T.

doi:10.1016/j.chroma.2007.06.020

Cited by 89 publications

(69 citation statements)

References 18 publications

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“…Besides icarrin (4), epimedin A (1), epimedin B (2), epimedin C (3), and baohuoside Ⅰ (5) are usually co-existing in Yinyanghuo [5]. The main bioactive compounds in Panax notoginseng (Sanqi in Chinese) are saponins, including notoginsenoside R1 (6), ginsenoside Rgl (7), and ginsenoside Rbl (8), which can antagonize thrombosis, dilate blood vessel, and protect cardiac microvessels [6,7]. Therefore, an accurate measurement of bioactive components, e.g., flavonoids and saponins, in these products becomes essential for quality control of their therapeutic efficacy.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the prevailing methods for determining flavonoids in Yinyanghuo and its herbal preparations are high-performance liquid chromatography-diode array detector (HPLC-DAD) or ultra-performance liquid chromatography (UPLC)-DAD [8,9]. On the other hand, HPLC-ELSD [10][11][12][13] is usually used for quantification of saponins in Sanqi owing to the low UV absorptivities of saponins.…”

Section: Introductionmentioning

confidence: 99%

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Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu¹,

Jia²,

Cai³

et al. 2013

Acta Chromatographica

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Summary. Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min −1 . The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu¹,

Jia²,

Cai³

et al. 2013

Acta Chromatographica

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show abstract

“…Besides icarrin (4), epimedin A (1), epimedin B (2), epimedin C (3), and baohuoside I (5) are usually co-existing in Yinyanghuo [5]. The main bioactive compounds in P. notoginseng (Sanqi in Chinese) are saponins, including notoginsenoside R1 (6), ginsenoside Rgl (7), and ginsenoside Rbl (8), which can antagonize thrombosis, dilate blood vessel, and protect cardiac microvessels [6,7]. Therefore, an accurate measurement of bioactive components, e.g., flavonoids and saponins in these products, becomes essential for quality control of their therapeutic efficacy.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the prevailing methods for determining flavonoids in Yinyanghuo and its herbal preparations are high-performance liquid chromatography−diode array detector (HPLC−DAD) or ultra-performance liquid chromatography (UPLC)−DAD [8,9]. On the other hand, HPLC-evaporative light scattering detector (ELSD) [10][11][12][13] is usually used for quantification of saponins in Sanqi owing to the low UV absorptivities of saponins.…”

Section: Introductionmentioning

confidence: 99%

Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active Flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu¹,

Jia²,

Cai³

et al. 2012

Acta Chromatographica

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Summary. Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of Herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography−diode array detector−evaporative light scattering detector (HPLC−DAD−ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile−water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min −1 . The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC−DAD−ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

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“…Among the state-of-the-art extraction methods used for extracting bitter compounds from hops, the most widespread is supercritical fluid extraction with carbon dioxide [8][9][10][11]14 . Another modern method, pressurized fluid extraction (PFE), has so far been employed for extracting different representatives of polyphenols 7,[15][16][17] . Our aim was to develop a less laborious and reliable procedure for extraction of bitter acids using pressurized solvent extraction (PSE).…”

Section: Introductionmentioning

confidence: 99%

Extraction of Bitter Acids from Hops and Hop Products Using Pressurized Solvent Extraction (PSE)

Čulík

Jurková

Horák

et al. 2009

Journal of the Institute of Brewing

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The EBC method 7.7, currently used for determination of bitter acids in hop products, is a time‐consuming and laborious extraction technique. In this paper, our aim was to propose a new extraction method based on Pressurized Solvent Extraction (PSE) sometimes also called Pressurized Fluid Extraction (PFE) or Accelerated Solvent Extraction (ASE). Compared to conventional extractions, PSE offers a number of important benefits. PSE on OnePSE® automated extractor was used for extraction of α‐ and β‐acids from hops and hop products and the parameters influencing extraction efficiency and the influence of the sample preparation method were studied. The quantitative determination of α‐ and β‐acids in the extracts was accomplished by using an HPLC apparatus equipped with diode array detector. The experimental results were compared with those obtained by the standard EBC 7.7 method and the two methods were found to be fully compatible

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Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography

Cited by 89 publications

References 18 publications

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active Flavonoids and three main saponins by HPLC-DAD-ELSD

Extraction of Bitter Acids from Hops and Hop Products Using Pressurized Solvent Extraction (PSE)

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