Quality evaluation ofYanghuo Sanqitablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Abstract

Summary. Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min −1 . The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

Advances and challenges in ginseng research from 2011 to 2020: the phytochemistry, quality control, metabolism, and biosynthesis

“…The quality control of CPM, such as identifying the used ginseng species and determining the major ginsenosides, tends to be more difficult because of the chemical complexity. A total of 25 CPMs containing P. ginseng , red ginseng, P. quinquefolius , and(or) P. notoginseng , were qualitatively and/or quantitatively analyzed, with the information detailed in Table S6 † 306–326 . HPLC, 318,321,322 HPLC/IT-MS, 189,309,323 HPLC/QQQ-MS, 214,219 HPLC/QTOF-MS, 216,306,312,317 HPLc/QTrap-MS, 307,320 HPLC/LTQ-Orbitrap-MS, 190 UHPLC, 184,310 UHPLC/LTQ-Orbitrap-MS, 315 UHPLC/QQQ-MS, 216,220 UHPLC/QTOF-MS, 199,201,308,311,319 NIRS, 316 and NMR, 325 were utilized.…”

“…CPMs that contain P. notoginseng are diverse, such as Xuesaitong Injection, 323 Xinkeshu Capsule/Tablet/Pill, 191 Xueshuantong Injection, 271,319 Compound Xueshuantong Capsule, 209,320 Yanghuo Sanqi Tablet, 321 Shuxiong Tablet, 326 Sanqi Panax Notoginseng Injection, 322 Compound Danshen preparations, 220,325 Naoluo Xintong Decoction, 324 and Qishen Yiqi Dropping Pill. 221 The fingerprint profiles of 10 batches of Xuesaitong Injection were analyzed, which showed 27 characteristic saponins.…”