Determination of the triterpene glycosides in sea cucumbers by liquid chromatography with evaporative light scattering and mass spectrometry detection

Abstract

Holothurian triterpene glycosides possess various kinds of biological activities, including antifungal, cytotoxic, hemolytic, cytostatic, and immunomodulatory effects. In this study, a rapid extraction method of triterpene glycosides from sea cucumbers using a small column of C18 solid phase was first developed. Furthermore, a novel high-performance liquid chromatography method coupled with evaporative light scattering detection and electrospray ionization mass spectrometry was established for the determination of each triterpene glycosides from different sea cucumbers. Simultaneous separation of all kind of triterpene glycoside were achieved on a C18 column. A gradient of aqueous acetonitrile was applied, and the method was validated. The liquid chromatography method was applied to the online mass detection to identify the triterpene glycosides in the purified extraction of eight kinds of pulverized sea cucumber from the market of Qingdao, China. The negative mode of [M-H](-)/[M-Na](-) exclusively shown signals corresponding to the triterpene glycosides previously reported and the MS(2) product ions of those ions indicate the specific structure of each triterpene glycoside.

Liquid chromatography with tandem mass spectrometry method for the simultaneous determination of multiple sweet mogrosides in the fruits ofSiraitia grosvenoriiand its marketed sweeteners

“…. It is well known that LC–MS/MS is an efficient tool for the determination of triterpene glycosides in crude extracts due to its rapid separation power, low solvent consumption, high sensitivity, high specificity, high selectivity, and multiple ion detection based on selective ion fragmentation .…”

Techniques for the analysis of pentacyclic triterpenoids in medicinal plants

“…CAD is a universal detector suitable for weak or non‐UV absorption compounds, which have been developed to overcome the limitations of traditional HPLC detection. HPLC–ELSD was used for the characterization and quantitation of triterpene glycosides in sea cucumbers , triterpenoid saponins in raw and sprouted Chenopodium berlandieri spp. , rosmarinic acid, maslinic acid, corosolic acid, betulinic acid, OA, and ursolic acid in the extracts of Prunella vulgaris L., due to its capacity for the sensitive detection of nonvolatile compounds containing weak chromophores .…”

Immunogenicity Analysis of Triterpene Glycoside From Holothuria Atra to Detecting Fas and BCL-2 Protein on the Sp-C1 Cell of Tongue Carcinoma

“…Qualitatively of triterpene glycoside was analyzed by high-performance liquid chromatography (also used to triterpenoid assay with added 50-100 mg/ml of triterpene glycoside in 0.5 ml of glacial acetate acid and incubated 15 min in tubes, and then added strong sulfate acid 0.5 ml). Positive has triterpene glycoside emerged brownish-red and purple colors [20,21].…”