Simultaneous determination of 10 components in traditional Chinese medicine Dachaihu Granule by reversed-phase-high-performance liquid chromatographic-diode array detector

Y, Hu; Lu, Tu-Lin; Mao, Chunqin; Wu, Hao; Zhang, Xing; Wang, JV; Gu, Juanjuan

doi:10.4103/0973-1296.108136

Cited by 8 publications

(3 citation statements)

References 3 publications

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“…The SFDA suggested that all herbal chromatograms should be evaluated in terms of similarity by calculating the correlation coefficient and/or angle cosine value of the original data. [ 16 17 18 ] Therefore, SA was conducted based on the mean peak area of standard fingerprints using correlation coefficient method and the cosine of the angle method to calculate the similarity of fingerprints. The same samples had similar constituents with the slight difference resulting from the BZZW and RZZW.…”

Section: Resultsmentioning

confidence: 99%

Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

et al. 2014

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Background:Traditional Chinese medicine (TCM) formula has been used for over 1000 years and most of them contain complicate chemical constituents. Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses for TCM. Zhi Zhu Wan (ZZW), a classical Chinese medical formula, has been commonly used for the treatment of gastrointestinal disease, which pose a serious challenge to its quality control.Materials and Methods:In this work, a sensitive and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) was developed to control the quality of ZZW for chemical fingerprint analysis and quantitative analysis of four major bioactive constituents, including hesperidin, naringin, neohesperidin, and atractylenolide I. The chromatographic separation was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm particle size), with an aqueous 0.095% phosphate acid and acetonitrile mobile phase gradient.Results:Optimization of other experimental conditions was validated with satisfactory accuracy, precision, repeatability, and recovery. In quantitative analysis, the four components showed good regression (R > 0.9994) within test ranges, and the recovery method ranged from 99.32% to 100.630%. HPLC fingerprints of the ZZW samples were compared by performing similarity analysis.Conclusion:The results indicated that the newly developed HPLC-PDA fingerprint method would be suitable for quality control of ZZW.

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Section: Resultsmentioning

confidence: 99%

Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

et al. 2014

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“…The herbal formulation abnormal savda munziq (ASMQ) tang of the traditional Uighur medicine from the Xinjiang region of China is used to treat complex diseases such as cancer, diabetes, and cardiovascular diseases. [ 1 2 3 4 5 ] This formulation is composed of 10 medicinal herbs, namely, Cordia dichotoma Forst. f, Anchusa italica Retz., Euphorbia humifusa Willd., Adiantum capillus-veneris L., Ziziphus jujube Mill., Glycyrrhiza uralensis , Foeniculum vulgare Mill., Lavandula angustifolia Mill., Dracocephalum moldavica L., and Alhagi pseudoalhagi Desv.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an high-performance liquid chromatography-diode array detector method for the simultaneous determination of six phenolic compounds in abnormal savda munziq decoction

Tian

Liu²,

Liu

et al. 2015

Phcog Mag

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Aims:Given the high-effectiveness and low-toxicity of abnormal savda munziq (ASMQ), its herbal formulation has long been used in traditional Uyghur medicine to treat complex diseases, such as cancer, diabetes, and cardiovascular diseases.Settings and Design:ASMQ decoction by reversed-phase high-performance liquid chromatography coupled with a diode array detector was successfully developed for the simultaneous quality assessment of gallic acid, protocatechuic acid, caffeic acid, rutin, rosmarinic acid, and luteolin. The six phenolic compounds were separated on an Agilent TC-C18 reversed-phase analytical column (4.6 × 250 mm, 5 μm) by gradient elution using 0.3% aqueous formic acid (v/v) and 0.3% methanol formic acid (v/v) at 1.0 mL/min.Materials and Methods:The plant material was separately ground and mixed at the following ratios (10): Cordia dichotoma (10.6), Anchusa italic (10.6), Euphorbia humifusa (4.9), Adiantum capillus-veneris (4.9), Ziziphus jujube (4.9), Glycyrrhiza uralensis (7.1), Foeniculum vulgare (4.9), Lavandula angustifolia (4.9), Dracocephalum moldavica L. (4.9), and Alhagi pseudoalhagi (42.3).Statistical Analysis Used:The precisions of all six compounds were <0.60%, and the average recoveries ranged from 99.39% to 104.85%. Highly significant linear correlations were found between component concentrations and specific chromatographic peak areas (R2 > 0.999).Results:The proposed method was successfully applied to determine the levels of six active components in ASMQ.Conclusions:Given the simplicity, precision, specificity, and sensitivity of the method, it can be utilized as a quality control approach to simultaneously determining the six phenolic compounds in AMSQ.

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“…Shaoyao-Gancao decoction (SGD) is a popular traditional Chinese medicine (TCM) and Shakuyaku-Kanzo-to in Japanese, firstly recorded in Shang Han Lun in Han Dynasty of Chinese history (202 BC-220 AD). [ 1 ] Its recipe is composed of two herbal medicines, Paeoniae Radix and Glycyrrhizae Radix , mainly used for the treatment of abdominal pain and dysmenorrhea disease. [ 2 ] Modern pharmacological studies have demonstrated that SGD carries various attractive biological activities.…”

Section: Introductionmentioning

confidence: 99%

Preliminary identification of the absorbed bioactive components and metabolites in rat plasma after oral administration of Shaoyao-Gancao decoction by ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry

et al. 2014

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Background:Shaoyao-Gancao decoction (SGD), a traditional Chinese medicine formula, has been used for the treatment of abdominal pain and dysmenorrhea disease in Asia over long period of time. Its effectiveness has been confirmed in clinic, but its active constituents remain unclear.Materials and methods:In this paper, a rapid, sensitive and reliable ultra-performance liquid chromatography-electrospray ionization/quadrupole-time-of-flight high-definition mass spectrometry (UPLC-ESI-Q-TOF-MS) in positive and negative ion mode were established to characterize the active constituents of SGD in vitro. The analysis was performed on a Waters UPLCTM HSS T3 (2.1 × 100 mm, 1.8 µm) using gradient elution system. Automated MetaboLynxTM technique was employed to screen for the potentially bioactive components in rat plasma after oral administration of SGD. MS/MS fragmentation behavior was proposed for aiding the structural identification of the components.Results:Based on the developed method of fingerprint analysis, an injection run of the plasma sample was finished in 15.0 min. A total of 12 compounds including 9 prototype components such as gallicacid, albiflorin, liquiritin, pallidiflorin, liquiritigenin, isoLiquiritigenin, formononetin, isolicoflavonol, licoricone, C9H10O3 and 2 metabolites such as liquiritigenin-4’-O-glucuronide, formononetin glucuronide were identified or tentatively characterized. Of note, 3 ingredients were identified from Radix Paeoniae Alba, and 9 were from Radix Glycyrrhizae.Conclusion:The compounds found in dosed plasma could be the effective substances of SGT for treating dysmenorrheal, and may provide important experimental data for further pharmacological and clinical research of SGD. Furthermore, this work has demonstrated that the feasibility of the UPLC-ESI-Q-TOF-MS for rapid and reliable characterization of identification and structural elucidation of the chemical constituents and their metabolites from herbal medicines.

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Simultaneous determination of 10 components in traditional Chinese medicine Dachaihu Granule by reversed-phase-high-performance liquid chromatographic-diode array detector

Cited by 8 publications

References 3 publications

Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

Development and validation of an high-performance liquid chromatography-diode array detector method for the simultaneous determination of six phenolic compounds in abnormal savda munziq decoction

Preliminary identification of the absorbed bioactive components and metabolites in rat plasma after oral administration of Shaoyao-Gancao decoction by ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry

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