Simultaneous Determination and Quantitation of Diosmetin and Hesperetin in Human Plasma by Liquid Chromatographic Mass Spectrometry With an Application to Pharmacokinetic Studies

Mandal, Partha Sarathi; Dan, Shubhasis; Chakraborty, Soumya; Ghosh, Balaram; Saha, Chiranjit; Khanam, Jasmina; Pal, Tapan Kumar

doi:10.1093/chromsci/bmz015

Cited by 17 publications

(13 citation statements)

References 13 publications

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“…Ingested diosmin becomes diosmetin through Phase I metabolism through the intestinal wall, and then is either glucuronidated (primarily), sulfated, or methylated through Phase II metabolism in the liver. [37][38][39][40][41][42][43][44][45][46][47] Serum analysis on healthy individuals demonstrates negligible presence of the aglycone in plasma, and low sustained levels of the diosmetin conjugates (primarily glucuronides) in plasma, with t max of 2.3 hrs and t 1/2 ranging from 8-70 hrs. [38][39][40][41][42][44][45][46][47] Stachulski and Meng (2013) and Tranoy et al (2014) note that most glucuronides are rapidly eliminated by the kidneys, posing an apparent limitation to their efficacy.…”

Section: Poor Polyphenol Pk Perceptionmentioning

confidence: 99%

“…[37][38][39][40][41][42][43][44][45][46][47] Serum analysis on healthy individuals demonstrates negligible presence of the aglycone in plasma, and low sustained levels of the diosmetin conjugates (primarily glucuronides) in plasma, with t max of 2.3 hrs and t 1/2 ranging from 8-70 hrs. [38][39][40][41][42][44][45][46][47] Stachulski and Meng (2013) and Tranoy et al (2014) note that most glucuronides are rapidly eliminated by the kidneys, posing an apparent limitation to their efficacy. 48,49 Glucuronidation further reduces bioavailability to the intracellular compartment as the glucuronide moiety imparts a hydrophilicity that prevents cellular uptake.…”

Section: Poor Polyphenol Pk Perceptionmentioning

confidence: 99%

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Polyphenolic Promiscuity, Inflammation-coupled Specificity: Whether PAINs Filters Mask an Antiviral Asset

Sheridan¹,

Spelman

2022

Preprint

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The Covid-19 pandemic has elicited much laboratory and clinical research attention on vaccines, mAbs, and certain small-molecule antivirals against SARS-CoV-2 infection. By contrast, there has been comparatively little attention on plant-derived compounds, especially those that are understood to be safely ingested at common doses and are often commonly consumed in the diet. With broader scope for assays and trials against a diverse array of viral infections, we review elucidations of the pharmacokinetic mechanisms of polyphenolic compounds over the past two decades and survey their putative frequent-hitter behavior. Many polyphenols are indeed promiscuous binders, suggesting a candidate mechanism of non-specific inhibition combined with inflammation-targeting specificity. Since such a specificity mechanism combined with promiscuity poses a possible pathway of inhibiting viral replication uniquely in infected tissue, we highlight pre-clinical studies of polyphenol aglycones that reduce virion replication. It is hoped that greater awareness of the potential specificity of polyphenolic activation to sites of pathogenic infection will spur renewed research and clinical attention on assaying and trialing against several infectious viral diseases.

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Section: Poor Polyphenol Pk Perceptionmentioning

confidence: 99%

Section: Poor Polyphenol Pk Perceptionmentioning

confidence: 99%

Polyphenolic Promiscuity, Inflammation-coupled Specificity: Whether PAINs Filters Mask an Antiviral Asset

Sheridan¹,

Spelman

2022

Preprint

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“…Minorsky, 2002;Tramontano & Jouve, 1997), and other active molecules such as oxymatrine (a drug used to treat hepatitis B and tumors. Lu et al, 2003;Song et al, 2006), diosmetin (in food or medicine with anti-oxidant properties, anti-infective, and anti-shock functions Pallab et al, 2019), luotonin A (anti-tumor drug. González-Ruiz et al, 2010), α-humulene (hippone, sesquiterpene, with anti-inflammatory effect.…”

Section: Untargeted Metabolomics Profiling Of P Triticisoli Bj-18mentioning

confidence: 99%

Siderophore and indolic acid production by Paenibacillus triticisoli BJ-18 and their plant growth-promoting and antimicrobe abilities

Zhang

Ren

Wang

et al. 2020

PeerJ

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Paenibacillus triticisoli BJ-18, a N2-fixing bacterium, is able to promote plant growth, but the secondary metabolites that may play a role in promoting plant growth have never been characterized. In this study, untargeted metabolomics profiling of P. triticisoli BJ-18 indicated the existence of 101 known compounds, including N2-acetyl ornithine, which is the precursor of siderophores, plant growth regulators such as trehalose 6-phosphate, betaine and trigonelline, and other bioactive molecules such as oxymatrine, diosmetin, luotonin A, (-)-caryophyllene oxide and tetrahydrocurcumin. In addition, six compounds were also isolated from P. triticisoli BJ-18 using a combination of silica gel chromatography, sephadex LH-20, octadecyl silane (ODS), and high-performance liquid chromatography (HPLC). The compound structures were further analyzed by Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS), and Electronic Circular Dichroism (ECD). The six compounds included three classical siderophore fusarinines identified as deshydroxylferritriacetylfusigen, desferritriacetylfusigen, and triacetylfusigen, and three indolic acids identified as paenibacillic acid A, 3-indoleacetic acid (IAA), and 3-indolepropionic acid (IPA). Both deshydroxylferritriacetylfusigen and paenibacillic acid A have new structures. Fusarinines, which normally occur in fungi, were isolated from bacterium for the first time in this study. Both siderophores (compounds 1 and 2) showed antimicrobial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis, but did not show obvious inhibitory activity against yeast Candida albicans, whereas triacetylfusigen (compound 3) showed no antibiosis activity against these test microorganisms. Paenibacillic acid A, IAA, and IPA were shown to promote the growth of plant shoots and roots, and paenibacillic acid A also showed antimicrobial activity against S. aureus. Our study demonstrates that siderophores and indolic acids may play an important role in plant growth promotion by P. triticisoli BJ-18.

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“…"Liquid chromatography tandem mass spectrometry (LC-MS)" assay has also been established for the determination of HSP in combination with other bioflavonoids in its plant-based materials [20]. Various LC-MS-based assays were also applied for the determination of HSP in combination with other bioflavonoids in the biological fluids of rats and humans [21][22][23][24][25]. An "ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS)" method has also been established for the simultaneous analysis of HSP, neo-HSP, naringin, naringenin, meranzin hydrate, and hesperitin in rat plasma samples and plant extract [26].…”

Section: Introductionmentioning

confidence: 99%

“…In reported LC-MS and UPLC-MS methods of HSP analysis, the mobile phase used was the binary combination of acetonitrile-W or acetonitrile-buffers. Acetonitrile is a highly toxic solvent and hence reported LC-MS and UPLC-MS methods are not safe and sustainable for HSP analysis [20][21][22][23][24][25][26]. A fluorimetry method has also been established for the simultaneous estimation of HSP and diosmin in combined dosage form and human plasma samples via complex formation using terbium [27].…”

Section: Introductionmentioning

confidence: 99%

A Sustainable Reversed-Phase HPTLC Method for the Quantitative Estimation of Hesperidin in Traditional and Ultrasound-Assisted Extracts of Different Varieties of Citrus Fruit Peels and Commercial Tablets

et al. 2021

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Hesperidin (HSP) is a bioactive flavanone glycoside, present abundantly in the variety of citrus fruits. The environmental safety and sustainability of the reported analytical assays of HSP analysis have not been considered in the literature. Hence, a sensitive and sustainable “reversed-phase high-performance thin-layer chromatography (RP-HPTLC)” method has been developed and validated for HSP analysis in traditional (TE) and ultrasound-based (UBE) extracts of four different varieties of citrus fruit peels and its commercial tablet dosage forms. The binary combination of green solvents such as ethanol-water (50:50, v v−1) was used as the mobile phase. The detection of HSP was performed at 287 nm. The sustainable RP-HPTLC method was linear in 20–2000 ng band−1 range. The studied validation parameters, including accuracy, precision, robustness, sensitivity were acceptable for HSP analysis. The content of HSP in TE of four different varieties of citrus fruits including grapefruit peels (Citrus paradisi), mosambi peels (Citrus limetta), lemon peels (Citrus lemon), and orange peels (Citrus sinensis) was detected as 8.26, 6.94, 5.90, and 6.81% w w−1, respectively. The content of HSP in TE of commercial formulations A and B was detected as 5.31 and 5.55% w w−1, respectively. However, the content of HSP in UBE of grapefruit peels, mosambi peels, lemon peels, and orange peels was detected as 11.41, 8.86, 7.98, and 8.64% w w−1, respectively. The content of HSP in UBE of commercial formulations A and B was detected as 6.72 and 6.92% w w−1, respectively. The greenness score of the sustainable RP-HPTLC method was predicted as 0.83 using analytical GREEnness (AGREE) metric approach, indicated the excellent greenness profile of the RP-HPTLC method. UBE procedure for HSP was superior over its TE procedure. These observations and results suggested that the present RP-HPTLC method can be successfully used for the quantitative estimation of HSP in the variety of citrus fruit peels and its commercial formulations. In addition, this method is simple, rapid, precise, accurate, and economical compared to the reported analytical methods of HSP analysis. It is also safe and sustainable method due to the use of ethanol-water solvents systems, as both the solvents are green solvents compared to the solvents used in reported analytical methods of HSP analysis.

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Simultaneous Determination and Quantitation of Diosmetin and Hesperetin in Human Plasma by Liquid Chromatographic Mass Spectrometry With an Application to Pharmacokinetic Studies

Cited by 17 publications

References 13 publications

Polyphenolic Promiscuity, Inflammation-coupled Specificity: Whether PAINs Filters Mask an Antiviral Asset

Polyphenolic Promiscuity, Inflammation-coupled Specificity: Whether PAINs Filters Mask an Antiviral Asset

Siderophore and indolic acid production by Paenibacillus triticisoli BJ-18 and their plant growth-promoting and antimicrobe abilities

A Sustainable Reversed-Phase HPTLC Method for the Quantitative Estimation of Hesperidin in Traditional and Ultrasound-Assisted Extracts of Different Varieties of Citrus Fruit Peels and Commercial Tablets

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