Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

Pui, Chai Fung; Wong, Woan Chwen; Chai, Lay Ching; Nillian, Elexson; Ghazali, Farinazleen Mohamad; Cheah, Yoke Kqueen; Nakaguchi, Yoshitsugu; Nishibuchi, Mitsuaki; Radu, Son

doi:10.1016/j.foodcont.2010.05.021

Cited by 91 publications

(52 citation statements)

References 30 publications

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“…Salmonella and Campylobacter are the most frequently isolated foodborne pathogens, and are predominantly found in poultry, eggs and dairy products (Silva et al 2011). Other food sources that are involved in the transmission of Salmonella include fresh fruits and vegetables (Pui et al 2011). In general, food animals such as swine, poultry and cattle are the prime sources of Salmonella infections.…”

Section: Introductionmentioning

confidence: 99%

Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance

Eng

Pusparajah

Mutalib

et al. 2015

Frontiers in Life Science

682

482

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Salmonella is one of the most frequently isolated foodborne pathogens. It is a major worldwide public health concern, accounting for 93.8 million foodborne illnesses and 155,000 deaths per year. To date, over 2500 Salmonella serotypes have been identified and more than half of them belong to Salmonella enterica subsp. enterica, which accounts for the majority of Salmonella infections in humans. Salmonella infections that involve invasive serotypes are often life threatening, necessitating appropriate and effective antibiotic therapy. The emergence of multi-drug-resistant (MDR) Salmonella serotypes is having a great impact on the efficacy of antibiotic treatment, and an increasing prevalence of MDR strains may lead to an increase in mortality rates of Salmonella infections. Epidemiological studies indicate that MDR Salmonella serotypes are more virulent than susceptible strains, as reflected by increased severity and more prolonged disease in patients infected by MDR strains. Preventive measures have been proposed to eliminate the spread of Salmonella infection. While the maintenance of effective food hygiene and water sanitation remain the cornerstones, additional measures such as restriction of indiscriminate use of antibiotics in food animals are important. This review provides an overview of Salmonella infection, and discusses the nomenclature, pathogenesis, clinical manifestations, epidemiology and antibiotic resistance of Salmonella.

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Section: Introductionmentioning

confidence: 99%

Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance

Eng

Pusparajah

Mutalib

et al. 2015

Frontiers in Life Science

682

482

View full text Add to dashboard Cite

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“…Furthermore, the poultry production systems are designed to ensure the massive production of birds usually in confinement. This design is necessary to meet the high protein demand, it also promote the introduction and establishment of the Salmonella organisms and hence transmission to the consuming population [8,9].…”

Section: Introductionmentioning

confidence: 99%

Critical Segments in the Dissemination and Transmission of Salmonella Species from Poultry Production in Calabar, Nigeria

Yhiler¹,

Bassey²

2015

SJPH

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Salmonella species are ubiquitous enteric bacteria, its ability to survive at different stages of the poultry production and food processing chain has been frequently reported in recent years. The present study was undertaken to investigate the points in the segments involved in the production of poultry in Calabar as vital sources of dissemination and transmission of Salmonella species to humans. A total of 374 samples were collected from three study segments involved in the production of poultry in Calabar, within the period of August 2013 and May 2014. 170 samples were collected from different points in poultry environment which includes (feed from feeders, water from drinkers, litter/faeces from floor, abattoir reins and drag swab from wall); 136 samples were taken from the live birds (cloacal swab, gut, carcass and egg); and 68 samples were derived from poultry handlers (stool specimens and hand wash). The samples were collected aseptically and analysed for the presence of Salmonella species based on the ISO 6579:2002 involving standard bacteriological, biochemical and serologic techniques. The Chi-square, student t-test and simple descriptive statistics were used to analyse the data obtained in this study at 95% confidence level. Salmonella species were recovered from 221 (59.1%) of the examined samples involved in the production of poultry in Calabar. There was no significant statistical difference in the rate of recovery of Salmonella species from the poultry environmental segment (58.8%), bird segment (55.1%) and poultry handlers segment (67.6%) (P = 0.230), implying that these three segments are equally important in the maintenance, dissemination and transmission of Salmonella species. Salmonella species were recovered from all the study sample points implying that all the study poultry sample points are sources of Salmonella species. However, the highest recovery rate of Salmonella species was observed in the stool sample points of poultry handlers (91.2%) and poultry gut sample points (79.4%) which were significantly different from the rest of the poultry sample points (P = 0.021), implying that these two sample points are the major sources of Salmonella species and are critical in the dissemination and transmission of Salmonella species.

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“…If only direct PCR was to be performed on the cat/ dog food samples, it is possible for false positive results to occur due to the presence of dead cells. The incorporation of MPN method which allows only viable cells to grow can solve this problem by complementing the PCR method for accurate detection of L. monocytogenes [22]. The primers used in this study amplified a specific region present in the hly gene of L. monocytogenes.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Listeria monocytogenes in pet food

et al. 2018

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Background: Listeria monocytogenes is one of the commonly isolated foodborne pathogens which cause illness, and listeriosis is a disease caused by this pathogen in human beings. Pets that consume contaminated pet food diets can be colonized by L. monocytogenes without showing clinical signs making the pets a possible source of contamination in the household. This study aimed to detect and enumerate the presence of L. monocytogenes in pet food diets, namely cat and dog food.Result: A total of 32 samples consisting of wet food (25%), dry food (25%), treats (25%), and leftover household samples (25%) were examined for this study. The pet food diets were sampled from pet food shops, grocery stores, and households located in Kuching and Kota Samarahan. The analysis was conducted using the most probable numberpolymerase chain reaction (MPN-PCR). According to the results obtained from MPN-PCR, none of the samples were contaminated by L. monocytogenes. Conclusion:Being the first biosafety assessment of L. monocytogenes in pet food in Malaysia, this study can contribute to the building of a database regarding the potential contamination of pet food diets by L. monocytogenes.

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Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

Cited by 91 publications

References 30 publications

Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance

Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance

Critical Segments in the Dissemination and Transmission of Salmonella Species from Poultry Production in Calabar, Nigeria

Assessment of Listeria monocytogenes in pet food

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