Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

Zhang, Lening; Wang, Zhanfeng; Wen, Yuan; Shi, Jingwei; Wang, Jincheng

doi:10.1039/c4ay01818d

Cited by 21 publications

(6 citation statements)

References 20 publications

(24 reference statements)

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“…因此, 发展能够高效检测有机磷化合物的方法对于防范和治理有机磷化合物污染至关重要. 目前, 有机磷化合物的检测方法有酶联免疫法(ELISA) [2] 、气-质联用法(GC-MS) [3] 、酶抑制法、生物芯片技术、液-质联用法(LC-MS) [4] 、高效薄层检测法(HPTLC) [5] 、电化学法 [6] 及荧光方法 [7] 等.…”

Section: 关键词unclassified

A “turn on” fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

et al. 2017

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A highly sensitive "turn-on" fluorescent probe for bovine serum albumin protein detection and quantification based on AIE-active distyrylanthracene derivative SCIENCE CHINA Chemistry 56, 1234 (2013); A highly sensitive "turn-on" fluorescent probe for bovine serum albumin protein detection and quantification based on AIE-active distyrylanthracene derivative SCIENTIA SINICA Chimica 43, 1212 (2013); A turn-on fluorescent probe for Au 3+ based on rodamine derivative and its bioimaging application SCIENCE CHINA Chemistry 57, 1043 (2014); Ultrasensitive detection of aliphatic nitro-organics based on "turn-on" fluorescent sensor array SCIENCE CHINA Chemistry 59, 89 (2016); A near-infrared phosphorescent probe for Fbased on a cationic iridium(III) complex with triarylboron moieties

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Section: 关键词unclassified

A “turn on” fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

et al. 2017

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“…In recent years, methods using conventional techniques, such as gas chromatography [ 8 ], liquid chromatography [ 9 , 10 ], enzyme inhibition methods [ 5 ], immunoassays [ [11] , [12] , [13] ], and molecularly imprinted polymer (MIP) sensors [ 14 , 15 ], have been developed to detect OPs. Although these methods are effective for the analysis of OPs, there are still some disadvantages, including requiring time-consuming pretreatment of samples, expensive equipment, and skilled operators.…”

Section: Introductionmentioning

confidence: 99%

A dual-signal sensor for the analysis of parathion-methyl using silver nanoparticles modified with graphitic carbon nitride

Wan

Yan

et al. 2021

Journal of Pharmaceutical Analysis

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A highly sensitive and selective method was developed for both UV–vis spectrophotometric and fluorimetric determination of organophosphorus pesticides (OPs). This method used silver nanoparticles (AgNPs) modified with graphitic carbon nitride (g-C 3 N 4 ). The AgNPs reduced the fluorescence intensity of g-C 3 N 4 . Acetylthiocholine (ATCh) could be catalytically hydrolyzed by acetylcholinesterase (AChE) to form thiocholine, which induces aggregation of the AgNPs. This aggregation led to the recovery of the blue fluorescence of g-C 3 N 4 , with excitation/emission peaks at 310/460 nm. This fluorescence intensity could be reduced again in the presence of OPs because of the inhibitory effect of OPs on the activity of AChE. The degree of reduction was found to be proportional to the concentration of OPs, and the limit of fluorometric detection was 0.0324 μg/L (S/N = 3). In addition, the absorption of the g-C 3 N 4 /AgNPs at 390 nm decreased because of the aggregation of the AgNPs, but was recovered in presence of OPs because of the inhibition of enzyme activity by OPs. This method was successfully applied to the analysis of parathion-methyl in real samples.

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“…To date, immunoassays such as enzyme-linked immunosorbent assay (ELISA), fluorescence polarization immunoassay (FPIA), and chemiluminescence enzyme-linked immunoassay (CLEIA) are widely used to detect parathion, owing to their simplicity, high throughput, and high speed. Although these immunoassays are simple and rapid compared to traditional methods, their sensitivities are not satisfying the testing requirements in some cases .…”

Section: Introductionmentioning

confidence: 99%

Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

Ge¹,

Jin²,

Ma³

et al. 2019

J. Agric. Food Chem.

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A competitive sensitive bio-barcode immunoassay based on bimetallic nanozyme (Au@Pt: gold@platinum) catalysis has been designed for the detection of the pesticide parathion. Gold nanoparticles (AuNPs) were modified with single-stranded thiol oligonucleotides (ssDNAs) and monoclonal antibodies (mAbs) to form AuNP probes; magnetic nanoparticles (MNPs) were coated with ovalbumin (OVA)–parathion haptens as MNP probes, and bimetallic nanozyme (Au@Pt) nanoparticles functionalized with the complementary thiolated ssDNA were used as Au@Pt probes. The Au@Pt probes reacted with the AuNP probes through complementary base pairing. Further, parathion competed with MNP probes to bind the mAbs on the AuNP probes. Finally, the complex system was separated by a magnetic field. The released Au@Pt probes catalyzed a chromogenic system consisting of teramethylbenzidine (TMB). The bimetallic nanozyme-based bio-barcode immunoassay was performed on rice, pear, apple, and cabbage samples to verify the feasibility of the method. The immunoassay exhibited a linear response from 0.01 to 40 μg·kg–1, and the limit of detection (LOD) was 2.13 × 10–3 μg·kg–1. The recoveries and relative standard deviations (RSDs) ranged from 73.12 to 116.29% and 5.59 to 10.87%, respectively. The method was found to correlate well with data obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS). In conclusion, this method exhibits potential as a sensitive alternative method for the detection of a variety of pesticides, ensuring the safety of fruits and vegetables in agriculture.

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Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

Abstract: A heterologous indirect competitive enzyme-linked immunosorbent assay for the gross determination of parathion and imidacloprid residues was developed.

Cited by 21 publications

References 20 publications

A “turn on” fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

A “turn on” fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

A dual-signal sensor for the analysis of parathion-methyl using silver nanoparticles modified with graphitic carbon nitride

Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

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Simultaneous detection of parathion and imidacloprid using broad-specificity polyclonal antibody in enzyme-linked immunosorbent assay

Abstract: A heterologous indirect competitive enzyme-linked immunosorbent assay for the gross determination of parathion and imidacloprid residues was developed.

Cited by 21 publications

References 20 publications

A &ldquo;turn on&rdquo; fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

A &ldquo;turn on&rdquo; fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

A dual-signal sensor for the analysis of parathion-methyl using silver nanoparticles modified with graphitic carbon nitride

Competitive Bio-Barcode Immunoassay for Highly Sensitive Detection of Parathion Based on Bimetallic Nanozyme Catalysis

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Product

Resources

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A “turn on” fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides

A “turn on” fluorescent probe based on Tb(III) complex for the detection of organophosphorus pesticides