Simultaneous detection of multi-allergens in an incurred food matrix using ELISA, multiplex flow cytometry and liquid chromatography mass spectrometry (LC–MS)

Food Allergy

“…Buckwheat (Hirao et al 2005) Conventional 1 ppm Quantitative real-time PCR: accurate, specific, sensitive, quantitative, rapid (Kirsch et al 2009, Poms et al 2004 Targeted DNA fragment may not be allergen specific, need special equipment, need appropriate calibrators, DNA contamination possible (Cucu et al 2013, Kirsch et al 2009, Poms et al 2004, van Hengel 2007 (Cucu et al 2013, Gomaa & Boye 2015, Kirsch et al 2009, van Hengel 2007 Does not target allergen (Cucu et al 2013, Kirsch et al 2009, Poms et al 2004, van Hengel 2007 Black mustard ( 10-50 mg/kg…”

Foodomic investigations of food allergies

“…Limit of detection and limit of quantification of the MS approaches are in a femtomolar/atomolar range, what is comparable with antibody-based techniques [38,42]. The threshold levels of safety for food allergens are still under research [41]. Thus, currently there is zero tolerance.…”

“…Sensitivity, reliability and specificity of MS-based proteomic techniques frequently offer an important advantage over antibody and nucleic acid based techniques in the complex task of identifying and quantifying known allergens in food matrices [38][39][40][41]. However, still complicated measurements and frequently long time that is necessary for sample preparation and analysis are the reason that these techniques have some limitations regarding their application for routine high-throughput analyses [8].…”

Alergeni U Hrani – Prisustvo, Rizici I Upravljanje U Prehrambenoj Industriji

“…Enzyme-Linked Immunosorbent Assays (ELISA) imaju značajnu primjenu u kliničkoj dijagnostici, a u detekciji alergena u hrani veću primjenu imaju u posljednjih desetak godina [69,70]. Kitovi koji se komercijalno nude, međusobno se razlikuju prema pragu detekcije alergena (na primjer, prag detekcije za rezidua kikirikija se kreće od 0.5 ppm do 5 ppm).…”