Simultaneous Detection of Circulating OncomiRs from Body Fluids for Prostate Cancer Staging Using Nanographene Oxide

Hizir, Mustafa Salih; Balcioglu, Mustafa; Rana, Muhit; Robertson, Neil; Yigit, Mehmet V.

doi:10.1021/am504190a

Cited by 78 publications

(77 citation statements)

References 33 publications

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“…To study how miRNAs regulate AR signaling, it is necessary to identify their targets. [15] reported that miR-141-3p expression is at normal levels in early PCa, which is in accordance with our findings. Using deep-sequencing analysis, we found that miR-141-3p expression was decreased by 1.26-fold in early PCa compared to that in BPH (P < 0.05), and confirmed a similar 1.17-fold decrease in early PCa compared to that in BPH (P = 0.2053) by qRT-PCR.…”

Section: Discussionsupporting

confidence: 93%

miR-141-3p Suppresses Expression of Androgen Receptors and Functions as a Tumor Suppressor Gene in Prostate Carcinogenesis

Song¹,

Chen²,

Wang³

et al. 2017

IJCM

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Background: Prostate cancer (PCa) is a leading cause of tumor mortality in Western societies. In China, the PCa mortality rate is increasing yearly. Androgen receptors (ARs) and microRNAs (miRNAs) play central roles in prostate carcinogenesis and progression. Methods: To characterize the underlying molecular mechanisms, we compared the miRNA profiles of early PCa (G ≤ 7), advanced PCa (G > 7) and non-tumor prostate tissues using deep-sequencing. The target genes of differentially expressed miRNAs were predicted by bioinformatics analysis and confirmed by luciferase reporter assays and Western blot (WB) and quantitative reverse transcription-PCR (qRT-PCR)analyses. Finally, we performed in vitro functional studies by inducing or inhibiting miR-141-3p expression using an artificial mimic or inhibitor. Results: A computational search implicated the open reading frame (ORF) of AR mRNA as a potential miR-141-3p target site. The qRT-PCR, WB and luciferase reporter assays revealed a reverse regulatory effect of miR-141-3p on AR. Mutation of the potential miR-141-3p binding site in the AR ORF resulted in a loss of responsiveness to the corresponding miRNA. Moreover, miR-141-3p expression levels were unchanged in early PCas, but were obviously increased in advanced PCas. MiR-141-3p overexpression inhibited RWPE-1 cell proliferation, mobility, and prohibited the entry of cells into the G2-S-M phase; miR-141-3p inhibition had the inverse effects. At the same time, we tested miR-141-3p's functions in PC-3 and VCaP prostate cancer cell lines. Conclusions: Taken together, our results indicate that miR-141-3p targets AR and its downstream signaling pathways, and functions as a tumor suppressor miR in PCa carcinogenesis by suppressing cell growth and mobility, but the effect is not significant in maglinant PCas. MiR-141-3p is implicated as a novel therapeutic target for early PCa.

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Section: Discussionsupporting

confidence: 93%

miR-141-3p Suppresses Expression of Androgen Receptors and Functions as a Tumor Suppressor Gene in Prostate Carcinogenesis

Song¹,

Chen²,

Wang³

et al. 2017

IJCM

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“…This is expected to be a non- or minimally invasive approach to diagnose early-stage prostate cancer. 49 …”

Section: Diagnosis Of Systematic Diseases By Salivamentioning

confidence: 99%

Saliva in the diagnosis of diseases

et al. 2016

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Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects and digestion. With the rapid advancement in salivaomics, saliva is well recognized as a pool of biological markers. Saliva, as a non-invasive and safe source, could be a substitute for blood in the diagnosis and prognosis of diseases. This review summarizes the latest advancements in saliva-related studies and addresses the potential value of saliva in the early diagnosis of oral diseases, such as dental caries and periodontal disease, as well as cancer, diabetes and other systemic disorders. Saliva biomarkers range from changes in the biochemical indices of DNA, RNA and proteins to the diversification of microbiota structures. This study integrates data reported in the recent literature and discusses the clinical significance and prospects for the application of saliva in the early diagnosis of diseases, translational medicine and precision medicine.

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“…This is currently needed because the blood level of the gold standard marker for prostate cancer, PSA, do not always correlate with disease stage and aggressiveness of the malignancy [57]. For example, miR-21 is significantly elevated in the early stage, but not in advanced prostate cancer [58] and miR-16 is up-regulated in plasma of metastatic prostate cancer patients, but down-regulated in primary or metastatic prostate cancer tissues [59]. Additionally, other miRNAs have been reported to be detected in blood exosomes in metastatic prostate cancer patients [60][61][62][63].…”

Section: Exosomes and Prostasomesmentioning

confidence: 99%

Exosomal microRNAs in liquid biopsies: future biomarkers for prostate cancer

et al. 2017

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Prostate cancer is the second most diagnosed cancer in males in the world. Plasma quantification of prostate-specific antigen substantially improved the early detection of prostate cancer, but still lacks the required specificity. Clinical management of prostate cancer needs advances in the development of new non-invasive biomarkers, ameliorating current diagnosis and prognosis and guiding therapeutic decisions. microRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene expression at the post-transcriptional level. These miRNAs are expressed in the cells and are also present in cellderived extracellular vesicles such as exosomes. Exosomes have been shown to act as mediators for cell to cell communication because of the regulatory functions of their content. High levels of exosomes are found in several body fluids from cancer patients and could be a potential source of non-invasive biomarkers. In this review, we summarize the diagnostic and prognostic utility of exosomal miRNAs in prostate cancer.

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Simultaneous Detection of Circulating OncomiRs from Body Fluids for Prostate Cancer Staging Using Nanographene Oxide

Cited by 78 publications

References 33 publications

miR-141-3p Suppresses Expression of Androgen Receptors and Functions as a Tumor Suppressor Gene in Prostate Carcinogenesis

miR-141-3p Suppresses Expression of Androgen Receptors and Functions as a Tumor Suppressor Gene in Prostate Carcinogenesis

Saliva in the diagnosis of diseases

Exosomal microRNAs in liquid biopsies: future biomarkers for prostate cancer

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