Simultaneous detection of 22 toxic plant alkaloids (aconitum alkaloids, solanaceous tropane alkaloids, sophora alkaloids, strychnos alkaloids and colchicine) in human urine and herbal samples using liquid chromatography–tandem mass spectrometry

Identification and Quantification of Aconitines and Colchicine in Serum, Urine, and Plants using MonoSpin C18 and LC-MSMS

“…Although these results were comparable to those of previously reported methods [22,24,26,27], these previous methods showed extensive variability among sample volumes ranging from 1 to 4 mL. However, our methods use less than one-tenth volume compared to previous methods.…”

“…Previous studies have reported aconitines being simultaneously extracted from human serum and urine using an Oasis ® HLB cartridge and liquid-liquid extraction [22,24,28]. Although the extracted sample was analyzed using LC-MS/MS, dryness and a re-constitution procedure were required prior to injection into the instrument.…”

“…However, LC-MS has recently been employed for the determination of colchicine in body fluids [22][23][24][25][26]. Several sample preparation methods have been reported for colchicine from blood, urine, and plant including liquidphase extraction [17,24], solid-phase extraction (SPE) [22,26], QuEChERS [23], and protein precipitation [25].…”

Simultaneous analysis of tropane alkaloids in teas and herbal teas by liquid chromatography coupled to high‐resolution mass spectrometry (Orbitrap)

“…In the case of LC, MS is the predominant detection system used due to its high selectivity and sensitivity. Several methods for the analysis of TAs in Solanaceae plants [17][18][19], body fluids [20][21][22][23], cereals crops [8,19,24], and herbal tea and tea [12,19,20] are available, allowing the determination of these compounds at μg/kg or μg/L levels using LC-MS. None of the above methods analyzed more than seven TAs, except the study performed by Mulder [19], and most of them only quantified scopolamine and atropine.…”

Chemical compositional analysis of the Tamarix hispida aerial part extract obtained in ethanol solutions of different concentration

“…The analysis of literature data allows for a conclusion that plants of the Chenopodiaceae family are characterized by a high proportional content of nitrogen-containing compounds, for example, in plants of the Sueda genus, Halostachys and Ephedra are sources of alkaloids of the piperidine and pyrrole, pyrolidinium and morphine groups; however, the content of organic sulfur and organochlorine compounds in the Tamarix genus was not previously reported, although these classes are quite common in the Embryophytes [23,24,25,26,27,28,29,30,31,32,33,34]. In the studied extracts, a large number of aromatic and polyaromatic compounds have been identifi ed; in particular, phthalic acid esters, as well as a number of organic sulfur compounds and nitrogenuous heterocycles [35,36,37,38,39].…”