Simultaneous Cultivation of Human Endothelial–like Differentiated Precursor Cells and Human Marrow Stromal Cells on β-Tricalcium Phosphate

Abstract

MSC and EPC can be cultivated in combination on a fibronectin-coated beta-TCP, thereby partly maintaining their lineage typical gene expression. The results of the in vivo examinations suggest that beta-TCP combined with EPC and MSC can used as a suitable tool to foster bone healing.

BMP2 and VEGF promote angiogenesis but retard terminal differentiation of osteoblasts in bone regeneration by up-regulating <italic>Id1</italic>

“…Isolation and culture of EPCs and BMSCs from rat bone marrow Wistar male rats (3 -4 weeks) with weight of 130-140 g were used for isolating BMSCs and EPCs, as described previously [16]. Mononuclear cells (MNCs) from femurs and tibias bone marrow aspirate were isolated by using a Percoll (Sigma, St Louis, USA) density gradient separation method (1200 g for 20 min) at room temperature.…”

Oxygen Tension-Controlled Matrices with Osteogenic and Vasculogenic Cells for Vascularized Bone Regeneration In Vivo

“…[6][7][8] There is a growing need to develop tissue engineering strategies for bone regeneration throughout the graft (i.e., large-area bone regeneration) via graft vascularization, and such methods are potentially applicable for segmental and critical-sized bone defect repair. [9][10][11][12][13] Many cellular-based bone tissue engineering approaches involve the use of bone marrow-derived mesenchymal stem cells (MSCs) as osteoprogenitor cells to promote and accelerate bone regeneration. 2,14,15 In this approach, MSCs are seeded on scaffolds for colonization and implantation at a defect site.…”

Mesenchymal stem cell (MSC) and endothelial progenitor cell (EPC) growth and adhesion in six different bone graft substitutes

“…A solution proposed in several studies is to promote early vascularization into bone graft substitutes by adding new vessel-forming cells such as endothelial progenitor cells (EPC) [10][11][12][13]. Early EPC are derived presumably from monocytic/dendritic precursors [14].…”