Simultaneous coupling of capillary supercritical fluid chromatography and high temperature glass capillary gas chromatography to a mass spectrometer

Abstract

SummaryThe coupling of glass capillary supercritical fluid chromatography to a high temperature GC/MS system via a micrometeradjustable glass capillary interface including an integrated pressure restrictor is described. With this coupling device, both complementary capillary chromatographic methods retain their full independence and flexibility.It is shown that in supercritical fluid chromatographyglassfulfils all the requirements of a suitable support material. The preparation of narrow bore glass capillary columns (0.06 mm i.d.),coatedwithchemicallybondedandcrosslinkedfluidswitha film thickness of about 0.6 p, which exploit the merits of OHterminated polysiloxanes as stationary phases is discussed. The application of glass capillary SFC is demonstrated and compared with examples given in the literature.

Estado da arte da cromatografia gasosa de alta resolução e alta temperatura

“…A principal razão para o aumento rápido no número de biomarcadores identificados nos últimos anos é devido à melhoria das técnicas analíticas, em particular a CGAR acoplada a espectrometria de massas (CGAR-EM) 68 . A CGAR-AT acoplada a espectrometria de massas por ionização em campo ("FIMS"), também foi aplicada largamente à análise de porfirinas e hidrocarbonetos aromáticos e saturados de alta massa molecular [69][70] . Mas além do aumento rápido no número de biomarcadores identificados, os novos biomarcadores têm maior massa molecular 61,68 .…”

Direct analysis of diphenylmethane polyisocyanates by mean of supercritical fluid chromatography/negative chemical ionization mass spectrometry

“…A more detailed description of this arrangement is given in ref. [6] The mass spectrometer was a Finnigan 4600 quadrupole instrument with enhanced pumping capacity [two Varian M-4 oil diffusion pumps (pumping capacity, 800 l/s/unit), two Edwards E2M40 rotary pumps (pumping capacity, 600 l/s/unit)l. The system was additionally coupled to a high temperature capillary GC [8, 91. In this work we demonstrate that direct assignment of di-and polyisocyanates is feasible by means of CSFC in combination with mass spectrometry (MS).…”