2014
DOI: 10.1039/c4lc00532e
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Simultaneous concentration and detection of biomarkers on paper

Abstract: The lateral-flow immunoassay (LFA) is an inexpensive point-of-care (POC) paper-based diagnostic device with the potential to rapidly detect disease biomarkers in resource-poor settings. Although LFA is inexpensive, easy to use, and requires no laboratory equipment, it is limited by its sensitivity, which remains inferior to that of gold standard laboratory-based assays. Our group is the only one to have previously utilized various aqueous two-phase systems (ATPSs) to enhance LFA detection. In those studies, th… Show more

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Cited by 48 publications
(37 citation statements)
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References 19 publications
(20 reference statements)
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“…25 However, the existing LFAs are associated with the limitation of poor sensitivity. 11,26 Several methods have been developed to enhance the sensitivity of LFAs, such as sample concentration, [27][28][29][30] thermal contrast, 31 fluidic control, [32][33][34] temperature-humidity technique, 35 probe-based signal enhancement, 23,36 enzyme-based signal amplification 37,38 and electrochemical devices-based enhancement. 39 However, these approaches require either external equipment, 35 high-cost reagents 23,37 or complicated fabrication with multistep procedure, 32 limiting their application for POC testing.…”
Section: Introductionmentioning
confidence: 99%
“…25 However, the existing LFAs are associated with the limitation of poor sensitivity. 11,26 Several methods have been developed to enhance the sensitivity of LFAs, such as sample concentration, [27][28][29][30] thermal contrast, 31 fluidic control, [32][33][34] temperature-humidity technique, 35 probe-based signal enhancement, 23,36 enzyme-based signal amplification 37,38 and electrochemical devices-based enhancement. 39 However, these approaches require either external equipment, 35 high-cost reagents 23,37 or complicated fabrication with multistep procedure, 32 limiting their application for POC testing.…”
Section: Introductionmentioning
confidence: 99%
“…Por esta razón cobra importancia la pre-concentración de la muestra, y diferentes mecanismos de pre-tratamiento están hoy bajo estudio (67). En particular se desea pre-concentrar en línea, es decir, en el propio dispositivo, para lo cual ya se están reportando ejemplos posibles (68). Pero un salto notable lo ha dado recientemente la pre-concentración en línea mediante isotacoforesis, al mostrar un incremento de la sensibilidad de dos órdenes de magnitud (69).…”
Section: Innovación Tecnológica De Los Dispositivosunclassified
“…Although the reason for this phenomenon is unclear, it is plausible that (1) the less viscous PEG-poor domains move ahead and coalesce quickly while the more viscous PEGrich domains remain behind and coalesce, and (2) the PEG molecules of the PEG-rich phase may have favorable interactions with the fibers of the paper, which further act to slow down the PEG-rich domains. 35 While the 1:1 solution showed noticeable separation on the paper strip, the phase separation for the 9:1 solution was less distinct. To further improve the phase separation, the authors implemented a 3D paper well consisting of layers of stacked paper (Fig.…”
Section: Concentration Of Biomoleculesmentioning
confidence: 99%
“…In an attempt to reduce the extraction time as well as user interaction, the Kamei group investigated combining the two processes directly in paper. 35 Using the PEG-salt ATPS, the authors applied a well-mixed ATPS solution containing the DGNPs and Tf directly onto a paper strip. The authors experimented with both 1:1 and 9:1 volume ratios; as before, the Tf-bound DGNPs partitioned into the PEG-poor phase.…”
Section: Concentration Of Biomoleculesmentioning
confidence: 99%
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