Simultaneous column chromatographic extraction and purification of abscisic acid in peanut plants for direct HPLC analysis

Zhang, Yawen; Fan, Wenlai; Li, Hui; Ni, He; Hanbing, Han; Li, Haihang

doi:10.1016/j.jchromb.2015.08.033

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Abscisic acid (ABA), benzoic acid (BA), gibberellic acid (GA3), indole acetic acid (IAA), jasmonic acid (JA), salicylic acid (SA), zeatin (Z), zeatin riboside (RZ), and isipentenyl adenine (IP) in the tested extracts was calculated on the basis of a calibration curve determined for each identified hormone. All samples were filtered through 0.22 µm membrane filters before injection into HPLC [ 101 , 102 ].…”

Section: Methodsmentioning

confidence: 99%

Investigation of Chemical Constituents and Antioxidant Activity of Biologically Active Plant-Derived Natural Products

et al. 2023

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The aim of this publication is to present rapid screening methods (visual/colorimetric) that will enable quick identification of the presence of biologically active compounds in aqueous solutions. For this reason, 26 plant extracts obtained by ultrasound-assisted extraction were analysed for the content of these compounds. Higher plants, used as a raw material for extraction, are common in Europe and are easily available. The article proposes a comparison of various protocols for the identification of various compounds, e.g., phenolic compounds (phenols, tannins, anthocyanins, coumarins, flavones, flavonoids), vitamin C, quinones, quinines, resins, glycosides, sugars. Initial characterisation of the composition of plant extracts using fast and inexpensive methods allows you to avoid the use of time-consuming analyses with the use of advanced research equipment. In addition, the antioxidant activity of plant extracts using spectrophotometric methods (DPPH, ABTS, FRAP assay) and quantitative analysis of plant hormones such as abscisic acid, benzoic acid, gibberellic acid, indole acetic acid, jasmonic acid, salicylic acid, zeatin, zeatin riboside, and isipentenyl adenine was performed. The obtained results prove that the applied visual methods show different sensitivity in detecting the sought chemical compounds. Therefore, it is necessary to confirm the presence or absence of bioactive substances and their concentration using modern analytical methods.

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Section: Methodsmentioning

confidence: 99%

Investigation of Chemical Constituents and Antioxidant Activity of Biologically Active Plant-Derived Natural Products

et al. 2023

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“…The technique completely extracted plant endogenous ABA in a pure and highly concentrated form for direct HPLC measurement by combining column chromatographic extraction and solid-phase separation technologies in one step. (13) In order to swiftly and accurately detect endogenous ABA and its five catabolites in fresh Oryza sativa tissues, a one-step hydrophilic interaction chromatography-based solid phase extraction (HILIC SPE) pretreatment method with silica as the sorbent was developed. Using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), the isolated analytes were sensitively measured.…”

Section: Extraction Process Of Abscisic Acidmentioning

confidence: 99%

“…With a 99.3% recovery rate, ABA in plant materials was fully extracted, purified, and analysed by HPLC in a 2 mL solution. The technique completely extracted plant endogenous ABA in a pure and highly concentrated form for direct HPLC measurement by combining column chromatographic extraction and solid-phase separation technologies in one step (13). In order to swiftly and accurately detect endogenous ABA and its five catabolites in fresh Oryza sativa tissues, a one-step hydrophilic interaction chromatography-based solid phase extraction (HILIC SPE) pretreatment method with silica as the sorbent was developed.…”

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Abscisic Acid in Treating Neurodegenerative and Other Diseases- An Overview

2023

IRJMETS

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Neurodegenerative diseases arise when neurons in the brain or the peripheral nervous system gradually lose function and die. Millions of people globally are affected by neurodegenerative diseases. With age, the chance of getting a neurodegenerative disease increases dramatically. Many of your body's functions are affected by degenerative nerve diseases, including balance, motion, speaking, respiration, and heart function. Alzheimer's disease (AD) is among the most prevalent type of dementia in the elderly. Abscisic acid (ABA) is a plant hormone found in fruits and vegetables that can be obtained naturally through diet and has previously been investigated for its potential health benefits. It has antioxidant and anti-inflammatory effects, and it improves cognitive learning and memory function. ABA has been shown to play an important part in the metabolism of glucose, inflammation, memory, and tumor growth. This review focuses on ABA's novel and promising role as a possible treatment for a variety of neurodegenerative disorders such as Alzheimer's and ADHD. We also included studies on illnesses such as diabetes mellitus and murine allergic airway inflammation. All of the studies referred to in this review demonstrate that ABA is effective in treating these diseases.

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Simultaneous Determination of Seven Plant Growth Regulators in Melons and Fruits by Modified QuEChERS Coupled with Capillary Electrophoresis

Chen¹,

et al. 2018

Food Anal. Methods

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Simultaneous column chromatographic extraction and purification of abscisic acid in peanut plants for direct HPLC analysis

Cited by 5 publications

References 26 publications

Investigation of Chemical Constituents and Antioxidant Activity of Biologically Active Plant-Derived Natural Products

Investigation of Chemical Constituents and Antioxidant Activity of Biologically Active Plant-Derived Natural Products

Abscisic Acid in Treating Neurodegenerative and Other Diseases- An Overview

Simultaneous Determination of Seven Plant Growth Regulators in Melons and Fruits by Modified QuEChERS Coupled with Capillary Electrophoresis

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