Simultaneous Chronoamperometric Sensing of Ascorbic Acid and Acetaminophen at a Boron-Doped Diamond Electrode

Abstract

Cyclic voltammetry (CV) and chronoamperometry (CA) have been used to sense and determine simultaneously L-ascorbic acid (AA) and acetaminophen (AC) at a boron-doped diamond electrode (BDDE) in a Britton-Robinson buffer solution. The calibration plots of anodic current peak versus concentration obtained from CV and CA data for both investigated compounds in single and di-component solutions over the concentration range 0.01 mM – 0.1 mM proved to be linear, with very good correlation parameters. Sensitivity values and RSD of 2-3% were obtained for various situations, involving both individual and simultaneous presence of AA and AC. The chronoamperometric technique associated with standard addition in sequential one step and/or two successive and continuous chronoamperograms at two characteristic potential levels represented a feasible option for the simultaneous determination of AA and AC in real sample systems such as pharmaceutical formulations. The average values indicated by the supplier were confirmed to a very close approximation from chronoamperomgrams by using several additions with the application of suitable current correction factors.

Rapid and Reliable HPLC Method for the Determination of Vitamin C in Pharmaceutical Samples

“…Many analytical techniques including sensors and biosensors [4][5][6] have been suggested for the detection of ascorbic acid in various types of samples. Integrated methods, utilizing flow injection analysis, high performance liquid chromatography [7][8][9] or capillary electrophoresis [10][11][12][13] and a detector, are mostly employed for the determination of vitamin C. However, some of these methods are time-consuming, while others are costly, require special training for operators of the equipment, or suffer from insufficient sensitivity or selectivity.…”

Anodic Determination of Acetylsalicylic Acid at a Mildly Oxidized Boron-Doped Diamond Electrode in Sodium Sulphate Medium

“…The detailed investigation using the chronoamperometry (CA) method, similar to our former study [57] at a fixed potential and sufficient time for a conventionally quasistationary state, was conducted to establish several working conditions and calibration plots data available as support for applications in practical analytical purposes, that is, the determination of ASA content in real samples obtained from pharmaceutical formulations. Figure 5 shows a series of chronoamperograms regarding the effect of ASA concentration on the useful anodic response at unmodified BDDE, using standard solutions in 0.1 M Na 2 SO 4 (pH 7) supporting electrolyte.…”

“…International Journal of Electrochemistry Boron-doped diamond (BDD) is a very important material for electroanalysis, since it exhibits several electrochemically valuable properties such as its very wide electrochemical window in aqueous solutions resulting from the high overpotentials of the oxygen and hydrogen evolution reactions, low and stable voltammetric and amperometric background current, its excellent chemical and electrochemical stability in aggressive conditions, and its good responsiveness to a range of analytes without any conventional pretreatment [49][50][51][52][53][54][55][56][57][58][59].…”

Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers

“…[26][27][28] Hyphenated instruments consisting of flow injection analysis, [29][30][31][32] high performance liquid chromatography [33][34][35] or capillary electrophoresis [36][37][38][39] instruments and a detector are mostly utilized for the determination of Vitamic C. Liquid chromatography is a successful method for vitamin C determination when selectivity and specificity are concerned. [40][41][42] The objective of the present study was to determine the relative bioavailability of synthetic Vitamin C and Nutra-C ® in Korean healthy volunteers.…”