Simultaneous characterization of pancreatic stellate cells and other pancreatic components within three-dimensional tissue environment during chronic pancreatitis

Hu, Wenyan; Fu, Ling

doi:10.1117/1.jbo.18.5.056002

Cited by 10 publications

(6 citation statements)

References 44 publications

(68 reference statements)

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“…3) [33], while chronic gastritis or gastric cancer will increase the infiltration of inflammatory cells, which mainly consists of lymphocytes [41]. Lymphocytes within gastric lamina propria and pancreas with chronic inflammation have been identified by their oval shape and higher fluorescence intensity at the wavelength range of 510-550 nm [22,23,42], consistent with the features of the yellow inflammatory cells that we observed (pink arrows in Fig. 4).…”

Section: Spectrum-coded 3d Structure Of Gastric Mucosasupporting

confidence: 82%

Spectrum- and time-resolved endogenous multiphoton signals reveal quantitative differentiation of premalignant and malignant gastric mucosa

Li¹,

Li²,

He³

et al. 2018

Biomed. Opt. Express

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Early identification of premalignant and malignant gastric mucosa is crucial to decrease the incidence and mortality of stomach cancer. Spectrum- and time-resolved multiphoton microscopy are capable of providing not only structural but also biochemical information at the subcellular level. Based on this multidimensional imaging technique, we performed a systematic investigation on fresh human tissue specimens at the typical stages of gastric carcinogenesis, including normal, chronic gastritis with erosion, chronic gastritis with intestinal metaplasia, and intestinal-type adenocarcinoma. The results demonstrate that this technique is available to characterize the three-dimensional subcellular morphological and biochemical properties of gastric mucosa and further provide quantitative indicators of different gastric disorders, by using endogenous contrast. With advances in multiphoton endoscopy, it has the potential to allow noninvasive, label-free, real-time histological and functional diagnosis of premalignant and malignant lesions of stomach in the future.

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Section: Spectrum-coded 3d Structure Of Gastric Mucosasupporting

confidence: 82%

Spectrum- and time-resolved endogenous multiphoton signals reveal quantitative differentiation of premalignant and malignant gastric mucosa

Li¹,

Li²,

He³

et al. 2018

Biomed. Opt. Express

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“…Recent studies [22] have reported that Langergans isles maintain their structure despite the necrotic surrounding, due to two main factors: the resistant collagen pseudo-capsule and the peripheral vascularization (figure 5).…”

Section: Resultsmentioning

confidence: 99%

Pancreatic Acinar and Island Vascular Apparatus Associated with Acute and Chronic Inflammatory Processes

Mateescu¹,

Oprea²,

Stanca³

et al. 2015

Acute and Chronic Pancreatitis

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“…Це процес загоєння після травми ПЗ, що не є патологічним [3]. Повторні епізоди пошкодження тканин (при ХП) і безперервна стимуляція панкреатичних зірчастих клітин профіброгенними стимулами можуть призве-сти до стійкої активації цих клітин і панкреатичного фіброзу, що сприятиме розвитку органної недостат-ності при ХП [4,5].…”

Section: вступunclassified

Functional Features of the Pancreas during Chronic Pancreatitis

Shevchenkо

Babiy

Кленіна

et al. 2021

GASTRO

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Aim of study: to identify functional status of pancreas in patients with chronic pancreatitis depending on the stage of fibrotic transformation and activity of parenchymal inflammation. Materials and methods. The preoperative laboratory results and the data of morphological study performed on biopsies obtained during surgery in 88 patients with complications of chronic pancreatitis (CP) were compared. According to morphological study of pancreatic biopsies, the early pancreatic parenchymal fibrosis (I–II degree) has been diagnosed in 31 cases (35.2 %), late fibrosis (III–IV degree) — in 57 (64.8 %) cases. Results. Active parenchymal inflammation at early and late stages of pancreatic fibrosis are associated with changes in pancreatic secretion and intracellular activation of pancreatic enzymes, leading to autodigestion and release of these enzymes from acinar cells into the blood, which has been confirmed by an increase of trypsin and amylase activity in blood compared with inactive inflammation. At late stages of fibrosis reduction of fecal elastase‑1 (161.7 ± 4.8) mg/g was observed in 73.9 % of patients. Reverse correlation has been found between fibrotic transformation of pancreatic parenchyma and the level of fecal elastase‑1 (r = –0.46; p = 0.05), i.e. progressing fibrotic transformation of pancreas leads to reduced exocrine function. Correlation analysis showed that the progression of fibrotic transformation violates carbohydrate metabolism — a direct correlation with glycosylated hemoglobin (r = 0.496; p = 0.05), namely, the endocrine secretion deficiency. Novelty in science. The pancreas functioning was studied depending on the inflammation activity and stage of fibrosis transformation of its parenchyma at chronic pancreatitis. The active inflammation of the pancreas was found to be accompanied by significant activation of pancreatic enzymes (significant increase in blood amylase and trypsin activity), which causes the progression of fibrotic transformation of pancreas in CP. Conclusions. Progression of fibrotic transformation of the pancreatic parenchyma in CP leads to deterioration of the exocrine pancreatic function, as defined by a decrease in the level of fecal elastase‑1 in case of late fibrosis of the pancreas and to the deterioration of endocrine pancreatic function, as manifested by impaired fasting glucose metabolism both at early and late stages of fibrosis, the development of pancreatogenic type II diabetes at the early stages of pancreatic fibrotic transformation, insulin resistance and type I diabetes at later stages of pancreatic fibrotic transformation.

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Simultaneous characterization of pancreatic stellate cells and other pancreatic components within three-dimensional tissue environment during chronic pancreatitis

Cited by 10 publications

References 44 publications

Spectrum- and time-resolved endogenous multiphoton signals reveal quantitative differentiation of premalignant and malignant gastric mucosa

Spectrum- and time-resolved endogenous multiphoton signals reveal quantitative differentiation of premalignant and malignant gastric mucosa

Pancreatic Acinar and Island Vascular Apparatus Associated with Acute and Chronic Inflammatory Processes

Functional Features of the Pancreas during Chronic Pancreatitis

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