Simultaneous characterization of isoflavonoids and astragalosides in two Astragalus species by high‐performance liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry

Abstract

A method was developed for the simultaneous identification of astragalosides (AGs) and isoflavonoids (IFs) in the roots of Astragalus membranaceus and Astragalus mongholicus by HPLC coupled with atmospheric pressure chemical ionization MS/MS (HPLC-APCI-MS/MS). Diagnostic fragment ions of AGs and different group of IFs were obtained with one AG and eight IF standards analyzed by CID-MS, which were adopted as characteristic MS/MS fingerprints for further identification of these compounds in the two Astragalus species by using HPLC-APCI-MS/MS. A total of 20 IFs and 10 AGs were identified or tentatively identified. Among them, six IFs were detected in A. membranaceus for the first time and five IFs were firstly identified in A. mongholicus. The results indicate that HPLC-APCI-MS/MS is a powerful tool for the simultaneous characterization of IFs and AGs in complex matrix.

Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC–DAD–ESI–MS/MS

“…Additionally, 4, just like compound 1, had UV absorptions with maxima at 260 and 290 nm. Based on these data, the structure of 4 was proposed to be 6 00 -O-acetylcalycosin-7-O-b-D-glucopyranoside [5].…”

“…By examining the known pterocarpane glucosides in Radix astragali, only 9,10-dimethoxypterocarpane-3-O-b-D-glucopyranoside and 3,9-dimethoxypterocarpane-10-O-b-D-glucopyranoside were found and are consistent with the above data. According to the content difference and the retention time in HPLC reported before [3][4][5][6][7][8][9][10], compounds 3 and 6 were therefore tentatively identified as 3,9-dimethoxypterocarpane-10-O-b-D-glucopyranoside and 9,10-dimethoxypterocarpane-3-O-b-D-glucopyranoside, respectively. Compound 4 exhibited a [M ?…”

“…Additionally, 5, just like compound 13, had UV absorptions with maxima at 250 and 301 nm. Based on these data [3][4][5][6][7][8][9][10], the structure of 5 was identified as ononin.…”

“…Therefore, methods to identify the effective substances are in great demand [2]. Recently, high-performance liquid chromatography-mass spectrometry (HPLC-MS n ) has proved to be a modern, powerful tool for the identification of substances in TCM extracts [3][4][5][6][7][8][9][10][11]. This approach has an advantage in terms of sensitivity and specificity.…”

Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC‐DAD‐ELSD and HPLC‐TOF/MS

“…Therefore, multi-bioactive marker compounds are quite necessary and reasonable for the quality evaluation of the more complicated herbal prescription. Chemical investigations and pharmacological studies on BYHWD have revealed that the constituents of BYHWD are numerous and diverse, including flavonoids, monoterpene glycosides, saponins and organic acids (Sheng et al, 2005;Ma et al, 2002;Zhang et al, 2007;Yang et al, 2006;Dong et al, 2007). In order to evaluate this prescription effectively and comprehensively and to ensure its correct clinical application, the simultaneous quantification of these types of compounds is a reasonable and advisable approach to controlling the quality of BYHWD.…”