Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC–DAD–ESI–MS/MS

Tang, Li; Liu, Ying; Wang, Yeling; Long, Chunlin

doi:10.1007/s11418-009-0381-1

Cited by 16 publications

(11 citation statements)

References 14 publications

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“…Radix Astragali is also commonly used as immunomodulating agent to stimulate the immune system of immunodeficient patients. Moreover, it has been reported that herbal formulations containing Radix Astragali and some of its constituents could produce hepatoprotective [24], antiviral [25] and antioxidative effects [26]. Recently, evidence from various animal and clinical studies has demonstrated that Radix Astragali may possess anticarcinogenic property [27], which could attenuate the systemic side effects of conventional antineoplatic drugs [28].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells

Wang

Xing

et al. 2013

Diagn Pathol

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BackgroundAstragalus memebranaceus is a traditional Chinese herbal medicine used in treatment of common cold, diarrhea, fatigue, anorexia and cardiac diseases. Recently, there are growing evidences that Astragalus extract may be a potential anti-tumorigenic agent. Some research showed that the total saponins obtained from Astragalus membranaceus possess significant antitumorigenic activity. Gastric cancer is one of the most frequent cancers in the world, almost two-thirds of gastric cancer cases and deaths occur in less developed regions. But the effect of Astragalus membranaceus on proliferation, invasion and apoptosis of gastric cancer BGC-823 cells remains unclear.MethodsAstragalus saponins were extracted. Cells proliferation was determined by CCK-8 assay. Cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of BGC-823 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.ResultsThe results demonstrated that total Astragalus saponins could inhibit human gastric cancer cell growth both in vitro and in vivo, in additional, Astragalus saponins deceased the invasion ability and induced the apoptosis of gastric cancer BGC-823 cells.ConclusionsTotal Astragalus saponins inhibited human gastric cancer cell growth, decreased the invasion ability and induced the apoptosis. This suggested the possibility of further developing Astragalus as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent in gastric cancer therapy.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells

Wang

Xing

et al. 2013

Diagn Pathol

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“…A. mongholicus has been broadly utilized as a principal herbal medicine in China, including Northeast, North, and Northwest China, as well as in Mongolia and Korea [ 3 ]. Based on pharmacological studies and clinical practice, the dried roots of A. mongholicus have good potential therapeutic applications, including vital-energy tonification, abscess drainage, diuretic properties, skin reinforcement, and tissue regeneration [ 4 – 6 ]. Recent studies revealed that these bioactivities are mainly associated with isoflavonoids and triterpenoid saponins [ 7 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge

et al. 2021

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Background Astragalus mongholicus Bunge is an important medicinal plant used in traditional Chinese medicine. It is rich in isoflavonoids and triterpenoid saponins. Although these active constituents of A. mongholicus have been discovered for a long time, the genetic basis of isoflavonoid and triterpenoid saponin biosynthesis in this plant is virtually unknown because of the lack of a reference genome. Here, we used a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing to identify genes involved in the biosynthetic pathway of secondary metabolites in A. mongholicus. Results In this study, NGS, SMRT sequencing, and targeted compound analysis were combined to investigate the association between isoflavonoid and triterpenoid saponin content, and specific gene expression in the root, stem, and leaves of A. mongholicus. Overall, 643,812 CCS reads were generated, yielding 121,107 non-redundant transcript isoforms with an N50 value of 2124 bp. Based on these highly accurate transcripts, 104,756 (86.50%) transcripts were successfully annotated by any of the seven databases (NR, NT, Swissprot, KEGG, KOG, Pfam and GO). Levels of four isoflavonoids and four astragalosides (triterpenoid saponins) were determined. Forty-four differentially expressed genes (DEGs) involved in isoflavonoid biosynthesis and 44 DEGs from 16 gene families that encode enzymes involved in triterpenoid saponin biosynthesis were identified. Transcription factors (TFs) associated with isoflavonoid and triterpenoid saponin biosynthesis, including 72 MYBs, 53 bHLHs, 64 AP2-EREBPs, and 11 bZIPs, were also identified. The above transcripts showed different expression trends in different plant organs. Conclusions This study provides important genetic information on the A. mongholicus genes that are essential for isoflavonoid and triterpenoid saponin biosynthesis, and provides a basis for developing the medicinal value of this plant.

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“…At present, AR is mainly used in the treatment of nephritis, diabetes and cancer, and the saponins, flavonoids and polysaccharides contained in the herb are considered as the main components contributing to its clinical therapeutic effect [1,9]. Thus, these compounds have been tentatively assigned as the chemical markers for the quality control of AR, and a variety of analytical methods have been developed for characterizing the flavonoids and saponins of AR [[10], [11], [12], [13], [14], [15], [16]]. Among them, high performance liquid chromatography (HPLC) is more frequently used.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of twenty-five compounds in different parts of Astragalus membranaceus var. mongholicus and Astragalus membranaceus by UPLC-MS/MS

Guo

Zhu

et al. 2019

Journal of Pharmaceutical Analysis

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As a traditional Chinese medicine, the root of Astragalus membranaceus var. mongholicus (AMM) or A. membranaceus (AM) has been widely used in China and other Asian countries for thousands of years. Till now, the flavonoids, phenolic acids and saponins are considered as the main active components contributing to their therapeutic effect in these plants. In order to clarify the distribution and contents of these compounds in different organs of these plants, a rapid and sensitive analytical method for simultaneous determination of 25 active compounds including seven types (i.e. dihydroflavones, isoflavane, isoflavones, flavones, pterocarpans, phenolic acid and saponins) within 10 min was established using ultra-pressure liquid chromatography coupled with tandem mass spectrometry (UPLC–MS/MS). Then, the established method was fully validated and successfully applied to the determination of the contents of these analytes in different parts (root, rhizome, stem, leaf and flower) of AMM and AM. The results indicated that the contents of the same type of compounds in two different species plants were significantly different. Moreover, the obvious differences were also found for the distribution and contents of different type of compounds in five organs of the same species. The present study could provide necessary information for the rational development and utilization of AMM and AM resource.

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Phytochemical analysis of an antiviral fraction of Radix astragali using HPLC–DAD–ESI–MS/MS

Cited by 16 publications

References 14 publications

RETRACTED ARTICLE: Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells

RETRACTED ARTICLE: Astragalus saponins affect proliferation, invasion and apoptosis of gastric cancer BGC-823 cells

Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge

Comparative analysis of twenty-five compounds in different parts of Astragalus membranaceus var. mongholicus and Astragalus membranaceus by UPLC-MS/MS

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