Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device

Ramadan, Qasem; Samper, Victor; Poenar, Daniel Puiu; Zhu, Liang; Chen, Yu; Lim, Teck Guan

doi:10.1016/j.snb.2005.04.018

Cited by 51 publications

(37 citation statements)

References 16 publications

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“…Therefore, it is often necessary to preconcentrate cells prior to lysis. 21,23 Cell trapping and concentration in microfluidic devices may take place in a contact or contactless manner. In the former case, cells have been immobilized onto a surface by chemical, 24 hydrodynamic, 25 or microelectrode-based dielectrophoretic trapping.…”

Section: Introductionmentioning

confidence: 99%

“…In the former case, cells have been immobilized onto a surface by chemical, 24 hydrodynamic, 25 or microelectrode-based dielectrophoretic trapping. 23,[26][27][28] For contactless trapping, various force fields have been utilized to concentrate cells in a suspension such as acoustic, 29,30 electric, 31,32 magnetic, 33 or optical 34 forces. Additionally, insulator-based dielectrophoresis ͑DEP͒ has been recently demonstrated to continuously concentrate cells in front of posts, ridges, or droplets inside a microchannel.…”

Section: Introductionmentioning

confidence: 99%

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Integrated electrical concentration and lysis of cells in a microfluidic chip

et al. 2010

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Lysing cells is an important step in the analysis of intracellular contents. Concentrating cells is often required in order to acquire adequate cells for lysis. This work presents an integrated concentration and lysis of mammalian cells in a constriction microchannel using dc-biased ac electric fields. By adjusting the dc component, the electrokinetic cell motion can be precisely controlled, leading to an easy switch between concentration and lysis of red blood cells in the channel constriction. These two operations are also used in conjunction to demonstrate a continuous concentration and separation of leukemia cells from red blood cells in the same microchannel. The observed cell behaviors agree reasonably with the simulation results.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrated electrical concentration and lysis of cells in a microfluidic chip

et al. 2010

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“…Photolithographic techniques enable fabrication of almost any desired MET geometry; as a result the geometry of an MET can be tuned for a specific application. Ramadan et al [18,[21][22][23][24][25][26][27] and Lee et al [20,28] have demonstrated customized MP traps which emphasize the effects of different MET geometries. Simulations of magnetic fields produced by different MET geometries have magnetic field profiles attributed to the shape of the MET and magnetic coupling between conductors or loops.…”

Section: Geometrymentioning

confidence: 99%

Applications of microelectromagnetic traps

Basore

Baker

2012

Anal Bioanal Chem

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Microelectromagnetic traps (METs) have been used for almost two decades to manipulate magnetic fields. Different trap geometries have been shown to produce distinct magnetic fields and field gradients. Initially, microelectromagnetic traps were used mainly to separate and concentrate magnetic material at small scales. Recently such traps have been implemented for unique applications, for example filterless bioseparations, inductive heat generation, and biological detection. In this review, we describe recent reports in which MET geometry, current density, or external fields have been used. Descriptions of recent applications in which METs have been used to develop sensors, manipulate DNA, or block ion current are also provided.

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“…Several microfluidic devices of this type have been introduced so far. [14][15][16][17][18] It has been reported that bacterial cells such as E. coli require a threshold field strength for lysis significantly higher than the one required for typical mammalian cells. 14 This is due to the presence of a peptidoglycan cell wall and their small size.…”

Section: Introductionmentioning

confidence: 99%

An electroactive microwell array for trapping and lysing single-bacterial cells

et al. 2011

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Interest in single-cell analysis has increased because it allows to understand cell metabolism and characterize disease states, cellular adaptation to environmental changes, cell cycles, etc. Here, the authors propose a device to electrically trap and lyse single-bacterial cells in an array format for high-throughput single-cell analysis. The applied electric field is highly deformed and concentrated toward the inside of the microwell structures patterned on the planar electrode. This configuration effectively generates dielectrophoretic force to attract a single cell per well. The microwell has a comparable size to the target bacterial cell making it possible to trap single cells by physically excluding additional cells. Inducing highly concentrated electric potential on the cell membrane can also effectively lyse the trapped single-bacterial cells. The feasibility of the authors' approach was demonstrated by trapping and lysing Escherichia coli cells at the single-cell level. The present microwell array can be used as a basic tool for individual bacterial cell analysis.

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Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device

Cited by 51 publications

References 16 publications

Integrated electrical concentration and lysis of cells in a microfluidic chip

Integrated electrical concentration and lysis of cells in a microfluidic chip

Applications of microelectromagnetic traps

An electroactive microwell array for trapping and lysing single-bacterial cells

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