Simultaneous and rapid determination of deoxynivalenol and its acetylate derivatives in wheat flour and rice by ultra high performance liquid chromatography with photo diode array detection

Xu, Jiaojiao; Zhou, Jianqin; Huang, Baifen; Cai, Zengxuan; Xu, Xia; Ren, Yiping

doi:10.1002/jssc.201501316

Cited by 21 publications

(12 citation statements)

References 34 publications

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“…The determined LOD and LOQ for all analytes were in the 1.78–15.0 and 5.87–49.5 µg/kg ranges, respectively. These low LOD and LOQ for DON and its modified mycotoxins were comparable with those disclosed in other publications [ 23 , 44 ], or even lower [ 45 ]. The calibration curves were linear over the calibration range for all compounds, resulting in R 2 values between 0.998 and 0.999.…”

Section: Resultssupporting

confidence: 90%

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Panasiuk

Jedziniak

Pietruszka

et al. 2020

Toxins

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A liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3Ac-DON), 15-acetyldeoxynivalenol (15Ac-DON), DON-3-glucoside (DON-3Glc) nivalenol and fusarenone-X in feedstuffs. Different techniques of sample preparation were tested: solid-liquid-extraction, QuEChERS, solid phase extraction with OASIS HLB columns or immunoaffinity columns and a Mycosep 225 Trich column. None of the six immunoaffinity columns tested showed cross-reactivity to all of the mycotoxins. Surprisingly, the results show that if the immunoaffinity columns bound 3Ac-DON, then they did not bind 15Ac-DON. The most efficient sample preparation was achieved with a Mycosep 225 Trich column clean-up. The chromatography was optimised to obtain full separation of all analytes (including 3Ac-DON and 15Ac-DON isomeric form). The validation results show the relative standard deviations for repeatability and reproducibility varied from 4% to 24%. The apparent recovery ranged between 92% and 97%, and the limit of quantification described a 1.30 to 50 µg/kg range. The method trueness was satisfactory, as assessed by a proficiency test and analysis of reference material. A total of 99 feed samples were analysed by the developed method, revealing the presence of DON and DON-3Glc in 85% and 86% of examined animal feeds, respectively at concentrations between 1.70 and 1709 µg/kg. The ratios DON-3Glc to DON in the surveyed feedstuffs were from a low of 3% to high of 59%.

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Section: Resultssupporting

confidence: 90%

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Panasiuk

Jedziniak

Pietruszka

et al. 2020

Toxins

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“…Previous studies mainly described the use of immunoaffinity columns (IACs), multifunctional columns (MFCs), and solid phase extractions (SPEs) in the clean-up procedure [25][26][27]. Considering that a large number of samples and 3 types of mycotoxins needed to be analyzed in our research, we were unwilling to employ SPE because this method requires relatively complex procedures and takes a long time.…”

Section: Optimization Of the Clean-up Procedure Chromatographic Columentioning

confidence: 99%

Natural Occurrence of Deoxynivalenol and Its Acetylated Derivatives in Chinese Maize and Wheat Collected in 2017

Yan

Liu

et al. 2020

Toxins

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Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyl-deoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. In this study, 579 wheat samples and 606 maize samples intended for consumption were collected from China in 2017 and analyzed to determine the co-occurrence of type-B trichothecenes (DON, 3-ADON, and 15-ADON). All the wheat samples tested positive for DON, while 99.83% of the maize samples were DON-positive with mean DON concentrations of 165.87 and 175.30 μg/kg, respectively. Per the Chinese standard limits for DON, 3.63% of wheat and 2.97% of the maize samples were above the maximum limit of 1000 μg/kg. The DON derivatives (3-ADON and 15-ADON) were less frequently found and were present at lower levels than DON in wheat. 3-ADON and 15-ADON had incidences of 13.53% and 76.40%, respectively, in maize. By analyzing the distribution ratio of DON and its derivatives in wheat and maize, DON (95.51%) was the predominant toxin detected in wheat samples, followed by 3.97% for the combination of DON + 3-ADON, while DON + 3-ADON + 15-ADON and DON + 15-ADON were only found in 0.17% and 0.35% of wheat samples, respectively. Additionally, a large amount of the maize samples were contaminated with DON + 15-ADON (64.19%) and DON (22.11%). The samples with a combination of DON + 3-ADON and DON + 3-ADON + 15-ADON accounted for 1.32% and 12.21%, respectively. Only one maize sample did not contain all three mycotoxins. Our study shows the necessity of raising awareness of the co-occurrence of mycotoxin contamination in grains from China to protect consumers from the risk of exposure to DON and its derivatives.

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“…For this purpose, a rice sample purchased from a local supermarket was ground into a fine powder. A recent published method based on a QuEChERS procedure has been applied for the extraction and clean‐up of DON from portions (5.0 g) of the rice powder. In short, the extraction was carried out with water and subsequently with acetonitrile.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Easy Detection of Deoxynivalenol on a Bismuth Oxide Screen‐printed Electrode

et al. 2016

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In this work we propose a rapid method for deoxynivalenol (DON) detection based on the differential pulse voltammetric (DPV) response of this mycotoxin on a bismuth oxide modified screen‐printed electrode (SPE). In alkaline media the reduction peak of Bi2O3 is modified and an additional peak at a more negative potential is observed due to the DON presence in solution. The experimental conditions and DPV instrumental parameters have been optimized in order to get adequate responses for DON analysis on the BiOx‐SPE devices. The best results are obtained in 0.10 mol L−1 KOH using a 60 mV s−1 scan rate and 40 mV pulse amplitude. The ratio of the DON peak current to the Bi(III) reduction peak current is used for quantification purposes of this mycotoxin. Two linear response ranges (r values >0.99) with a sensitivity of 0.0019 L μg−1 (5.5×105 L mol−1) in the lower concentration range have been obtained at these measurement conditions. The reproducibility of the method is adequate, with RSD values lower than 10 % using different SPE devices for each measurement. The LOD and LOQ values (7.1 μg L−1, 2.4×10−8 mol L−1 and 24 μg L−1, 8.1×10−8 mol L−1 respectively) permit to determine DON with adequate precision and accuracy at low concentration levels. These good analytical features and the disposable and economical devices and the portable and simple instrumentation used for the measurements could make possible the implementation of this methodology for the in‐situ determination of DON.

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Simultaneous and rapid determination of deoxynivalenol and its acetylate derivatives in wheat flour and rice by ultra high performance liquid chromatography with photo diode array detection

Cited by 21 publications

References 34 publications

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Simultaneous Determination of Deoxynivalenol, Its Modified Forms, Nivalenol and Fusarenone-X in Feedstuffs by the Liquid Chromatography–Tandem Mass Spectrometry Method

Natural Occurrence of Deoxynivalenol and Its Acetylated Derivatives in Chinese Maize and Wheat Collected in 2017

Rapid and Easy Detection of Deoxynivalenol on a Bismuth Oxide Screen‐printed Electrode

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