Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

Abstract

In this paper, a novel method of QuEChERS-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) in guava leaves. The QuEChERS-based extraction parameters, including the amount of added salt, vortex-assisted extraction time, and absorbent amount, and the chromatographic conditions were investigated for the analysis of UA and OA in guava leaves. Under the optimized conditions, the method showed good linearity over a range of 1–320 μg mL−1, with correlation coefficients above 0.999. The limits of detection of UA and OA were 0.18 and 0.36 μg mL−1, respectively. The intraday and interday precision were below 1.95 and 2.55%, respectively. The accuracies of the UA and OA determinations ranged from 97.4 to 111.4%. The contents of UA and OA in the guava leaf samples were 2.50 and 0.73 mg g−1, respectively. These results demonstrate that the developed method is applicable to the simultaneous determination of UA and OA in guava leaves.

Simultaneous Determination of α-Glucosidase Inhibitory Triterpenoids in Psidium guajava Using HPLC–DAD–ELSD and Pressurized Liquid Extraction

“…An HPLC-DAD method with evaluation using PCA and Neural Network Analysis has been developed for simultaneous determination of 13 phenolic bioactive compounds in P. guajava [29]. An HPLC-PDA method was established for quantification of ursolic acid and oleanolic acid in the P. guajava [30]. Our previous research developed an HPLC method for quantification of corosolic acid and found the content of corosolic acid in leaf increased after acid treatment due to the hydrolysable corosolic acid derivatives.…”

New QuEChERS method for quantification of Physalin B and D in Physalis angulata L. in Vietnam

“…This poses a requirement that a suitable impurities removal method be developed that can reduce impurities while still retaining the majority of the analyte. Among the extraction methods that can be used to extract organic compounds from medicinal and plant samples, the QuEChERS method is becoming increasingly popular (Malysheva et al 2020;Xu et al 2017). This method is based on the decrease in solubility of organic substances dissolved in water as the salt concentration in the aqueous solution increases.…”

Ursolic Acid and Its Derivatives as Bioactive Agents

“…Liposomal ursolic acid (LUA) was used as a fresh drug in normal young volunteers and in individuals with developed solid tumours in order to determine the highest permitted level (MTD), dose-limiting toxicities (DLTs), and UA pharmacokinetics. A total sample of LUA (11,22,37,56,74,98 and 130 mg/m2) was given to 63 subjects (4 patients, 35 healthy participants and 24 adults). For the first season, clinical information revealed that LUA had manageable 98 mg/m 2 MTD toxicity.…”