New QuEChERS method for quantification of Physalin B and D in Physalis angulata L. in Vietnam

Abstract

In Vietnam, Physalis angulata L. has been widely used as a traditional medicine to treat fever, anti-inflammatory, and expectorant. Currently, there have been studies on the content of chemical composition especially physalin with anti-diabetic, anti-inflammatory, antibacterial, prevent cancer. This study developed a reliable and sensitive method to determine and validate simultaneous Physalin B and Physalin D in Physalis angulata L.. The QuEChERS method was used for sample preparation from leaf matrices and quantified by using High-performance liquid chromatography coupled with a diode-array detector. The method of research was validated under AOAC and ICH guidance. Chromatography conditions include Agilent C18 column (250mm × 4,6mm; 5µm) with a gradient mode using acetonitrile – methanol-water as mobile phase. The recovery of the method ranged from 94.21 – 105.93% and RSD was from 1.20 – 2.31%, the LOD, and LOQ were 0.4 mg/kg – 2.4 mg/kg, respectively. The results of the study showed that the proposed the new QuEChERS method for quantification of Physalin B and D in Physalis angulata L. in Vietnam.

Factors affecting loneliness among left-behind children

“…First, LBC in this study have a lower loneliness score than LBC in other studies, especially in China (Ai & Hu, 2016;Cao et al, 2022;Zhao et al, 2015;Zhao et al, 2019). The score is comparable for the adolescent group in Vietnam studied by Nguyen et al (2020). There is no significant difference in loneliness among different sub-groups of LBC.…”

“…Among the CLS scales, perception of social support and parent-child attachment has been used in previous research for adolescents in Vietnam by Nguyen et al (2020), Dinh (2016) and Tran et al (2013), proving its reliability and efficacy. The resilience measurement scale was translated from Chinese into Vietnamese by a Vietnamese Ph.D., who is not a member of the research team.…”

“…In Vietnam, CLS has been used by Nguyen et al (2020) for children of mean age 14.89 (SD = 2.52). The results showed that CLS ensures internal consistency with a Cronbach's α of .85, split-half part 1 = 0.76 and part 2 = 0.81.…”