Simultaneous analysis of phospholipid in rabbit bronchoalveolar lavage fluid by liquid chromatography/mass spectrometry

Hayakawa, Jun-ichiro; Okabayashi, Yoshito

doi:10.1016/j.jpba.2004.02.007

Cited by 25 publications

(11 citation statements)

References 28 publications

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“…Only one paper has so far reported the quantitative analysis of different phospholipids in rabbit BALF by LC-MS [31]. Using normal phase HPLC/MS the authors quantify the total contribution of all the species inside one class.…”

Section: Discussionmentioning

confidence: 99%

LC–MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid

Barroso

Bischoff

2005

Journal of Chromatography B

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Section: Discussionmentioning

confidence: 99%

LC–MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid

Barroso

Bischoff

2005

Journal of Chromatography B

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“…Each class with the same polar head-group can be subdivided into molecular species on the basis of the structures of the esterified FAs (length of the alkyl chain and number of double bonds). As a consequence, PLs have large structural diversity and complexity [2].…”

Section: Introductionmentioning

confidence: 99%

Determination of phosphatidylethanolamine molecular species in various food matrices by liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS2)

et al. 2012

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A liquid chromatographic-electrospray ionization-tandem mass spectrometric (LC-ESI-MS(2)) method has been developed for determination of the molecular species of phosphatidylethanolamine (PE) in four food matrices (soy, egg yolk, ox liver, and krill oil). The extraction and purification method consisted of a pressurized liquid extraction procedure for total lipid (TL) extraction, purification of phospholipids (PLs) by adsorption on a silica gel column, and separation of PL classes by semi-preparative normal-phase HPLC. Separation and identification of PE molecular species were performed by reversed-phase HPLC coupled with electrospray ionization tandem mass spectrometry (ESI-MS(2)). Methanol containing 5 mmol L(-1) ammonium formate was used as the mobile phase. A variety of PE molecular species were detected in the four food matrices. (C16:0-C18:2)PE, (C18:2-C18:2)PE, and (C16:0-C18:1)PE were the major PE molecular species in soy. Egg yolk PE contained (C16:0-C18:1)PE, (C18:0-C18:1)PE, (C18:0-C18:2)PE, and (C16:0-C18:2)PE as the major molecular species. Ox liver PE was rich in the species (C18:0-C18:1)PE, (C18:0-C20:4)PE, and (C18:0-C18:2)PE. Finally, krill oil which was particularly rich in (C16:0(alkyl)-C22:6(acyl))plasmanylethanolamine (PakE), (C16:0-C22:6)PE, and (C16:0-C20:5)PE, seemed to be an interesting potential source for supplementation of food with eicosapentaenoic acid and docosahexaenoic acid.

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“…The separation of PLs on a normal phase LC (NPLC) column is mainly achieved based on the differences in the polar head groups of the PLs [12][13][14][15], whereas separation on a reversed phase LC (RPLC) column relies on the differences of chain length and the degree of unsaturation of the acyl chain [9,16]. Due to these limitations, single dimensional HPLC-ESI-MS alone has not provided a comprehensive analysis of complicated PL mixtures from biological organisms.…”

Section: Introductionmentioning

confidence: 99%

Shotgun analysis of phospholipids from mouse liver and brain by nanoflow liquid chromatography/tandem mass spectrometry

Bang

Ahn

Moon

2007

Journal of Chromatography B

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Simultaneous analysis of phospholipid in rabbit bronchoalveolar lavage fluid by liquid chromatography/mass spectrometry

Cited by 25 publications

References 28 publications

LC–MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid

LC–MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid

Determination of phosphatidylethanolamine molecular species in various food matrices by liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS2)

Shotgun analysis of phospholipids from mouse liver and brain by nanoflow liquid chromatography/tandem mass spectrometry

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