Determination of phosphatidylethanolamine molecular species in various food matrices by liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS2)

Zhou, Li; Zhao, Minjie; Ennahar, Saïd; Bindler, Françoise; Marchioni, Eric

doi:10.1007/s00216-012-5772-6

Cited by 29 publications

(23 citation statements)

References 30 publications

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“…Other authors (Zhou et al 2012b) analyzed the SM species in KO with LC-ESI-MS2, but they could not found any. The same authors in a different work reported the presence of eight different species of PE (Zhou et al 2012a). …”

Section: Phospholipid Molecular Speciesmentioning

confidence: 89%

Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

et al. 2015

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Krill oil represents an interesting source of bioactive lipid components, being suitable as a functional ingredient. This oil is characterized by its high concentration of longchain omega-3 fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The contents of EPA and DHA were similar to those in fish oils, but with the difference that almost the half are located in phospholipids (mainly phosphatidylcholine). This might explain its higher absorption and bioavailability. This highly unsaturated oil maintains stable due to the presence of astaxanthin, a potent antioxidant, which assures the stability of the omega-3 fatty acids. However, there is lack of investigations reporting a deep comprehensive description of the krill oil (KO) lipid composition. The characterization includes new data of its neutral and polar components and the identification of triacylglycerols, diacylglycerols, and molecular species that has been done by different chromatographic techniques as gas chromatography-mass spectrometry/flame ionization detector (GC-MS/ FID), flash chromatography-evaporative light scattering detector (FC-ELSD), and HPLC-ELSD. Also phospholipid molecular species by using ultraperformance liquid chromatography/quadruple-time-of-flight mass spectrometry (UPLC/QToF-MS) have been determined.

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Section: Phospholipid Molecular Speciesmentioning

confidence: 89%

Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

et al. 2015

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“…Egg PE consists primarily of saturated FA such as stearic acid (18:0) at the sn -1 position, with a balanced mixture of unsaturated FA at the sn -2 position. The major PE molecular species include PE (16:0/18:1), PE (18:0/18:1), PE (18:0/18:2), and PE 18:0/20:4 [ 28 , 29 , 30 ]. Egg SM contains primarily saturated FA with palmitic acid (16:0) and stearic acid (18:0) making up ~80% of SM FA.…”

Section: Phospholipid Content and Composition Of The Chicken Eggmentioning

confidence: 99%

“…Mono- and di-unsaturated PE may influence cholesterol absorption like SM, as it has been shown to display a similar affinity to cholesterol as SM [ 49 , 50 ]. Both 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoethanolamine (POPE) [ 49 ] and 1-stearoyl-2-linoleoyl- sn -glycero-3-phosphoethanolamine (SLPE) [ 50 ], major PE molecular species in egg yolk [ 30 ], have been shown to interact with cholesterol in monolayers to a similar degree as SM; this suggests an important role for PE in lipid raft formation at the inner membrane leaflet of cells where it is most abundant. Due to the high affinity for cholesterol observed with certain PE species, dietary PE from egg may influence the absorption of luminal cholesterol similar to dietary SM.…”

Section: Egg Phospholipids and Lipid Absorptionmentioning

confidence: 99%

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Egg Phospholipids and Cardiovascular Health

2015

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Eggs are a major source of phospholipids (PL) in the Western diet. Dietary PL have emerged as a potential source of bioactive lipids that may have widespread effects on pathways related to inflammation, cholesterol metabolism, and high-density lipoprotein (HDL) function. Based on pre-clinical studies, egg phosphatidylcholine (PC) and sphingomyelin appear to regulate cholesterol absorption and inflammation. In clinical studies, egg PL intake is associated with beneficial changes in biomarkers related to HDL reverse cholesterol transport. Recently, egg PC was shown to be a substrate for the generation of trimethylamine N-oxide (TMAO), a gut microbe-dependent metabolite associated with increased cardiovascular disease (CVD) risk. More research is warranted to examine potential serum TMAO responses with chronic egg ingestion and in different populations, such as diabetics. In this review, the recent basic science, clinical, and epidemiological findings examining egg PL intake and risk of CVD are summarized.

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“…To analyze the PL in krill oils, analytical methods based on liquid chromatography with tandem mass spectrometry can be used (Le Grandois et al, ; Zhou et al, , ). However, nuclear magnetic resonance (NMR) spectroscopy is of particular interest for the holistic quality control of PL and FA in krill oil (Burri et al, ).…”

Section: Introductionmentioning

confidence: 99%

³¹P NMR Method for Phospholipid Analysis in Krill Oil: Proficiency Testing—A Step toward Becoming an Official Method

Zailer¹,

Monakhova

Diehl³

2018

J Americ Oil Chem Soc

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A high-resolution 31 P NMR method for determining the concentration of phospholipid (PL) species in krill oil was proficiency tested using an international interlaboratory study. Fourteen laboratories from five countries tested five different krill oil samples providing 17 datasets. Krill oil samples were prepared by the organizer using liquid-liquid extraction and sent to the participants who ran the nuclear magnetic resonance (NMR) analysis and evaluated the spectra. The relative standard deviation (RSD) among all the results provided by the participants varied between 2.1% and 3.3% for the phosphatidylcholine (PtdCho) content and between 2.8% and 12.3% for the sum of other PL. Afterward, an automated procedure was applied to all collected NMR data, which significantly improved the RSD values and provided reliable quantitative results for specific PL (PtdCho, 1-lysophosphatidylcholine [LPC], 2-LPS, PtdEtn, acylphosphatidylethanolamine [APE], and lysophosphatidylethanolamine [LPE]). The RSD varied between 1.6% and 2.7% for PtdCho and between 1.6% and 2.9% for the total phosphorus. Higher but acceptable values were obtained for 1-LPC (5.1% to 8.9%), PtdEtn (7.0% to 10.5%), and LPE (5.7% to 16.5%) due to their small concentrations. The influence of different magnetic field strengths (400-600 MHz), probes, and slight differences in acquisition parameters was assessed as nonsignificant. PtdCho hydrolysis during 14 weeks before measurements was the major factor that affects the result distribution. This method was submitted to the AOCS to become an official AOCS method for krill oil analysis.

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Determination of phosphatidylethanolamine molecular species in various food matrices by liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS2)

Cited by 29 publications

References 30 publications

Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

Egg Phospholipids and Cardiovascular Health

³¹P NMR Method for Phospholipid Analysis in Krill Oil: Proficiency Testing—A Step toward Becoming an Official Method

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Determination of phosphatidylethanolamine molecular species in various food matrices by liquid chromatography–electrospray ionization–tandem mass spectrometry (LC–ESI–MS2)

Cited by 29 publications

References 30 publications

Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

Comprehensive Study of the Lipid Classes of Krill Oil by Fractionation and Identification of Triacylglycerols, Diacylglycerols, and Phospholipid Molecular Species by Using UPLC/QToF-MS

Egg Phospholipids and Cardiovascular Health

31P NMR Method for Phospholipid Analysis in Krill Oil: Proficiency Testing—A Step toward Becoming an Official Method

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Product

Resources

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³¹P NMR Method for Phospholipid Analysis in Krill Oil: Proficiency Testing—A Step toward Becoming an Official Method