Simultaneous analysis of hydrocortisone and hydrocortisone phosphate by high‐pressure liquid chromatography: Reversed‐phase, ion‐pairing approach

Korpi, J.; Wittmer, D.P.; Sandmann, Beverly J.; Haney, W.G.

doi:10.1002/jps.2600650738

Cited by 35 publications

(5 citation statements)

References 1 publication

(1 reference statement)

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“…The carboxylic acids can also be chromatographed as ion pairs with organic cations. Studies were made in the pH range [6][7][8] with symmetrical quaternary ammonium ions as counterions in the mobile phase.…”

Section: Resultsmentioning

confidence: 99%

“…Naphthalene acetic acid derivatives have been separated as ion pairs and as acids in reversed phase chromatographic systems using aqueous mobile phases containing methanol or acetonitrile (5). Separation of anionic solutes by similar systems after addition of quaternary ammonium compounds such as tetrapropylammonium, tetrabutylammonium, or tetrapentylammonium (6, 7) or cetrimide (8) has been reported. This paper reports on reversed phase chromatographic systems for separation of carboxylic acids in acidic form and as ion pairs using acetonitrile as organic component in the mobile phase and LiChrosorb RP-8 (10-µ particles) as hydrophobic solid phase.…”

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confidence: 99%

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Retention behavior of carboxylic acids and their quaternary ammonium ion pairs in reversed phase chromatography with acetonitrile as organic modifier in the mobile phase

Tilly-Melin¹,

Askemark²,

Wahlund³

et al. 1979

Anal. Chem.

167

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Retention behavior of carboxylic acids and their quaternary ammonium ion pairs in reversed phase chromatography with acetonitrile as organic modifier in the mobile phase

Tilly-Melin¹,

Askemark²,

Wahlund³

et al. 1979

Anal. Chem.

167

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“…Phenothiazine" 15. Methoxyphenamine• (HC1) 16. Diphenhydramine• (HC1) 17. Diphenylpyraline-(HC1) 18. Dimethoxanate•(HC1) 19. Promethazine•(HC1) 20 Predicting Separation Conditions.…”

Section: Methodsmentioning

confidence: 99%

“…High efficiency ion-pair partition chromatography of amines has been demonstrated using aqueous (11)(12)(13) and nonaqueous (11) stationary liquid phases supported on silica gel. Recently, octadecylsilyl bonded packings have been used as stationary phases for reverse-phase "ion pair" chromatography of cationic sample components using a large organic anion as the counterion (14,15), and for anionic sample components using quaternary ammonium counterions (15)(16)(17)(18). Horvath et al (19,20) have investigated the retention of charged species from 0003-2700/78/0350-0491$01.00/0 aqueous solutions on octadecylsilyl phases and interpreted the results in terms of "solvophobic theory".…”

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confidence: 99%

Liquid chromatographic analysis of pharmaceutical syrups using pre-columns and salt-adsorption on Amberlite XAD-2

Mohammed¹,

Cantwell²

1978

Anal. Chem.

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Twenty-nine drug compounds are chromatographed in acidic, neutral, and alkaline solutions containing varying amounts of water and methanol on a column of Amberlite XAD-2 nonionic resin. Cationic conjugate acids of amine drugs are adsorbed via "salt-adsorption". Their retention volumes depend on concentration and type of anion (e.g. Cl , CI04 ) added to the mobile phase as well as on its pH and solvent composition. Pharmaceutical syrups can be analyzed without prior treatment when short pre-columns containing anion-exchange resin and XAD-2 are added. As examples, synthetic and commercial syrups are analyzed for phenylephrine hydrochloride, acetaminophen, glyceryl guaiacolate, and dextromethorphan hydrobromide.

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“…It is used for treating various inflammatory conditions, lymphomas and for inducing precocious fetal lung maturation in threatened preterm labor. Several methods have been described for simultaneously determining hydrocortisone and some synthetic corticosteroids by high-performance liquid chromatography [8][9][10][11][12][13]. The goal of this study was to develop a method for the simultaneous analysis of fusidic acid and steroids from bulk drugs and human plasma, as they have same formulation, and there is no reported method for the determination of all these steroids and fusidic acid.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

Nawaz¹,

Arayne²,

Sultana³

et al. 2014

Acta Chromatographica

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Summary.A simple, rapid, and sensitive high-performance liquid chromatography method was developed and validated for the simultaneous determination and quantification of fusidic acid and steroids (prednisone, betamethasone valerate, hydrocortisone acetate, and dexamethasone sodium) from bulk drugs and human plasma. A RP-HPLC, operated at ambient temperature, was equipped with a UV detector for monitoring the effluents at 235 nm. The mobile phase consisted of methanol, acetonitrile, and 0.05 M phosphoric acid (10:60:30, v/v/v), and separation was achieved on a Medeterrane, C18 (5 µm, 12.5 x 0.46 mm) column at a flow rate of 1.7 mL min −1 . Calibration curves were linear over concentration range 0.625-10 μg mL −1 with correlation coefficient (r 2 ) greater than 0.9999. The coefficient of variation (CV) and relative error (RE) for intra-and interassay were <2% and <1%, respectively. Interference of other already administered common medicaments, such as aspirin, paracetamol, caffeine, nicotine, and other plasma components, were not found.

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Simultaneous analysis of hydrocortisone and hydrocortisone phosphate by high‐pressure liquid chromatography: Reversed‐phase, ion‐pairing approach

Cited by 35 publications

References 1 publication

Retention behavior of carboxylic acids and their quaternary ammonium ion pairs in reversed phase chromatography with acetonitrile as organic modifier in the mobile phase

Retention behavior of carboxylic acids and their quaternary ammonium ion pairs in reversed phase chromatography with acetonitrile as organic modifier in the mobile phase

Liquid chromatographic analysis of pharmaceutical syrups using pre-columns and salt-adsorption on Amberlite XAD-2

Simultaneous determination of fusidic acid and steroids from bulk drugs and human plasma by reversed phase HPLC

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