Simultaneous analysis of homocysteine and methionine in plasma

Jandík, Petr; Cheng, Jun; Evrovski, Jovan; Avdalović, Nebojša

doi:10.1016/s0378-4347(01)00219-5

Cited by 22 publications

(20 citation statements)

References 14 publications

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“…The voltammetric detection of Hcy was successfully performed for the first time in our study in the artificial urine medium, AU‐Siriraj by using disposable PGE. The single‐use graphite electrodes could bring many important advantages over the existing methods in the literature 15–20, 32–39, 41–46, such as, being easy to use resulting with a less time consuming and cheaper detection protocol of Hcy, even in the mixture samples containing Hcy with low molecular‐mass biothiols derivates; L ‐cysteine, L ‐methionine and glutathione. This study enabled to perform, simple and precise determination of homocysteine using the single‐use PGE and DPV technique.…”

Section: Discussionmentioning

confidence: 99%

Electrochemical Determination of Homocysteine at Disposable Graphite Electrodes

Ekşin

Erdem

2014

Electroanalysis

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In the case of disruption of Hcy metabolism, the blood level of Hcy increases and it causes particularly the cardiovascular diseases, cancer, dementia and Parkinson’s disease. Thus, the sensitive analysis of Hcy levels in biological fluids is very important. Hcy analysis was performed herein using very practical and cost‐effective protocol using differential pulse voltammetry and graphite electrode. Detection limit of Hcy was found to be 1.21 µM in the linear range from 2 µM to 20 µM. The electrochemical Hcy detection in artificial urine medium was also successfully performed even in the presence of L‐Cysteine, L‐Methionine and Glutathione.

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Section: Discussionmentioning

confidence: 99%

Electrochemical Determination of Homocysteine at Disposable Graphite Electrodes

Ekşin

Erdem

2014

Electroanalysis

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“…16 It is useful in the cure of AIDS associated myelopathy 17 and also cancer. 18 Human uids like blood plasma, 19,20 urine and serum contain Met. 21 An abnormal concentration of Met leads mainly to coronary artery disease in the human body 22 and hyper methioninemia and hyperhomocysteinemia in infants.…”

Section: Introductionmentioning

confidence: 99%

“…24,25 Levels of Met for a healthy human were determined to be $30.4 mM in human plasma, 19 $25.5 mM in human serum 21 and $5.9 mM in human urine. 21 A number of methods to detect Met have been described, including gas chromatography-mass spectrometry, 19,20 HPLC detection, 26 spectrophotometry 27 and conductometry. 28 However, limitations such as advanced devices, long time analysis and technical expertise are mentioned in connection with these methods.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Ru–Pt bimetallic monolayer on nanoporous gold film electrode and its application as an ultrasensitive sensor for determination of methionine

2017

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We report here the fabrication of ruthenium/platinum (RuPt) bimetallic monolayer coated on a nanoporous gold film electrode (RuPtNPGF) by underpotential deposition of copper (UPD) with the Cu layer then replaced spontaneously by Ru and Pt. The present method could provide a very low RuPt-loading electrode and the results demonstrated that ultra thin RuPt coating behaved efficiently as a bimetallic nanostructure RuPt for electrocatalytic oxidation of methionine. The structure and morphology of the catalyst were defined by using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM), respectively. The RuPtNPGF electrode displayed good performance along with low working potential, high selectivity, and a low detection limit for the oxidation of methionine. Whereas at the surface of the bare electrode a redox peak for methionine cannot be observed, a sharp anodic peak at a potential of 0.73 V (vs. Ag/AgCl) in pH ¼ 7.0 is obtained using the prepared RuPtNPGF electrode. Under optimized conditions differential pulse voltammetry (DPV) of methionine showed two linear ranges for the determination of methionine: 6-105 nmol L À1 and 3-102 mmol L À1 with a detection limit of 2 nmol L À1 . Finally, the proposed sensor was successfully applied for the determination of methionine in human urine, with satisfactory results.

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“…In addition, PED at a gold electrode has proven to be selective for sulfur-containing compounds under mildly acidic conditions [10,19,20]. The use of PED following HPLC has been applied to methionine [20,21], glutathione oxidized/reduced [10,22], cysteine [21,22], homocysteine [21,23], antibiotics including penicillins and cephalosporins [11,[24][25][26], and other reduced and oxidized sulfur-containing compounds.…”

Section: Introductionmentioning

confidence: 99%