Determination of thio-based additives for biopharmaceuticals by pulsed electrochemical detection following HPLC

Modi, Swati J.; LaCourse, William R.; Shansky, Richard E.

doi:10.1016/j.jpba.2004.08.042

Cited by 12 publications

(10 citation statements)

References 22 publications

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“…Hence, the contribution to the overall signal from the reduction of dissolved O 2 is minimized. On-line degassing as part of the solvent delivery system can relax constraints on the start/end potential [25]. The detection steps in both waveforms are followed by a large negative potential pulse (-2000 mV for 10 ms) to induce both cathodic cleaning of the electrode [26] and reduce any partly-soluble Au [27].…”

Section: Electrochemical Response and Waveform Optimizationmentioning

confidence: 99%

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Wang¹,

Lin²

2017

Mod Chem Appl

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A novel method for the determination of isopropyl-β-D-thiogalactopyranoside (IPTG), a sulfur-containing compound, a pharmaceutical additive in commercial recombinant human growth hormone (rhGH), has been developed using pulsed electrochemical detection (PED), following ion Chromatographic separation. A Dionex-500 ion chromatograph coupled with an electrochemical detector was employed, equipped with a gold working electrode and an Acclaim 300 analytical C18 column, with a mobile phase consisting of sodium acetate (NaOAc) buffer (pH 5.45, 0.01 mol/L) and acetonitrile (ACN)(90/10, v/v). Upon optimization, IPTG was found to have a limit of detection of 1 ng/mL (0.1 μmol) with 25 uL injection volume. This method was successfully applied to the determination of IPTG in rhGH samples with the characteristics of simplicity, high sensitivity and good repeatability.

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Section: Electrochemical Response and Waveform Optimizationmentioning

confidence: 99%

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Wang¹,

Lin²

2017

Mod Chem Appl

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“…Even though electrodes are not readily fouled, electrode surfaces do eventually deteriorate and need to be changed when analysts observe diminished signal strength of quality control samples. PEDs have been used to analyze pharmaceutical preparations with hydroxyl and sulfur-containing organic compounds, explosive-containing environmental specimens, and enantiomers (163,164). Kaushik et al (165) identified and quantified ethyl glucuronide, a direct metabolite of ethanol used to assess drinking status, in human urine; the authors proposed a generalized approach using PED assays to analyze drug glucuronides (166).…”

Section: Cyclic Voltammetry and Pulsed Electrochemical Detectors (Ped)mentioning

confidence: 99%

Modern Instrumental Methods in Forensic Toxicology

Smith

Vorce

Holler

et al. 2007

Journal of Analytical Toxicology

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This article reviews modern analytical instrumentation in forensic toxicology for identification and quantification of drugs and toxins in biological fluids and tissues. A brief description of the theory and inherent strengths and limitations of each methodology is included. The focus is on new technologies that address current analytical limitations. A goal of this review is to encourage innovations to improve our technological capabilities and to encourage use of these analytical techniques in forensic toxicology practice.

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“…Since decomposition products resulting from IPTG have an unpleasant thiol smell, IPTG solutions have to be put into light-protected bottles and carefully monitored. 11,12 Even so, IPTG's rapid clearance and short half-life limit its usage and present a major drawback of using this popular gene induction system in animal systems. 13−15 To circumvent this stability problem, our group has reported the design and synthesis of a new lac inducer, isobutyl-β-Cgalactoside (IBCG, 1), 16 as a C-glycoside analogue of IPTG.…”

Section: ■ Introductionmentioning

confidence: 99%

Multigram Synthesis of Isobutyl-β-C-galactoside as a Substitute of Isopropylthiogalactoside for Exogenous Gene Induction in Mammalian Cells

et al. 2012

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Herein we report that isobutyl-β-C-galactoside (IBCG) is also a promising inducer of gene expression in mammalian cells and report a new synthetic route to the compound that should make obtaining the multigram quantities of material required for animal studies more feasible. A convenient synthesis of IBCG, an inducer of genes controlled by the lac operon system in bacterial cells, was achieved in 5 steps from galactose in 81% overall yield without any chromatographic separation steps. An optimized microwave-assisted reaction at high concentration was key to making the C-glycosidic linkage. A Wittig reaction on a per-O-silylated rather than per-O-acetylated or -benzylated substrate proved most effective in installing the final carbon atom.

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Determination of thio-based additives for biopharmaceuticals by pulsed electrochemical detection following HPLC

Cited by 12 publications

References 22 publications

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Modern Instrumental Methods in Forensic Toxicology

Multigram Synthesis of Isobutyl-β-C-galactoside as a Substitute of Isopropylthiogalactoside for Exogenous Gene Induction in Mammalian Cells

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