Simultaneous Analysis of Guaiacol and Vanillin in a Vanilla Extract by Using High-Performance Liquid Chromatography with Electrochemical Detection

Abstract

We developed and validated a new high-performance liquid chromatographic analysis for electrochemically detecting guaiacol and vanillin as important components in vanilla extract. Separation was achieved with Capcell Pak C-18 MG, the potential of the working electrode being set at þ1000 mV. The respective calibration curves for guaiacol and vanillin were linear in the range of 1.60-460 g/L and 5.90-1180 g/L. The respective limits for the quantities of guaiacol and vanillin were 1.60 g/L and 2.36 g/L. The related standard deviations of the intra-and inter-day precision of the retention time and peak area were all less than 4%. The recovery of guaiacol and vanillin was both more than 97%, all of the validation data being within an acceptable range. This analysis method is well suited for the simultaneous and convenient analysis of guaiacol and vanillin in a vanilla extract to evaluate the quality of the vanilla extract.

Method development for simultaneous detection of ferulic acid and vanillin using high-performance thin layer chromatography

“…HPLC-ECD, a new method was developed for the analysis of phenolic compound. However, use of HPLC with ECD is not feasible as a routine analytical method (Takahashi et al 2013). Even with the HPLC UV detector system, the analysis is time consuming and the quantity of the solvents is the main concern.…”

Tahitian Vanilla (Vanilla ×tahitensis): A Vanilla Species with Unique Features

“…However, we were able to show that the strongest correlations were between phenolic, woody, smoky notes and a pool of phenolic compounds such as guaiacol and creosol. Such correlation between guaiacol and woody notes has also been highlighted for V. planifolia [25] and led to the development of more appropriate analysis methods to characterize vanilla flavour also based on the detection of negative compounds such as guaiacol [30]. Anisyl compounds, especially methyl anisate were well correlated with anise notes.…”

A colorimetric assay for vanillin detection by determination of the luminescence of o-toluidine condensates

“…In a study on milk powder analysis, sample preparation took over 1 hour[ 26 ]. HPLC remains the most dominant technique for the analysis of vanillin, but such methods also have disadvantages, such as the considerable time required for column equilibration[ 17 ]. Therefore, an extraction technique should be rapid, simple and inexpensive.…”

“…There are several methods for vanillin detection, such as liquid chromatography-mass spectrometry (LC-MS)[ 11 , 12 ], capillary electrophoresis (CE)[ 13 ], electrochemical methods[ 14 – 16 ], high-performance liquid chromatography (HPLC)[ 17 ], and gas chromatography-mass spectrometry (GC-MS)[ 18 ]. In the past ten years, LC-MS has been widely used for the determination of vanillin[ 12 , 19 , 20 ] but has suffered from a few disadvantages, such as its complicated and time-consuming nature and poor recovery.…”