Simultaneous analysis of biochemical markers in vitreous humour and serum: a preliminary study on the effect of storage time at –20°C

Gürler, Mukaddes; Öztürk, Gülfer; Kır, Muhammed Ziya; Giniş, Zeynep; Erden, Gönül; Akyol, Sümeyya; Kaya, Mehmet; Karapırlı, Mustafa; Akyol, Ömer

doi:10.1080/00450618.2015.1042046

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…The analysis of Mg, Ca, total bilirubin, creatinine, AST, urea, total protein showed no significant differences with the increase in PMI. Similar results of these parameters after death were also found for humans (GURLER et al, 2016). Nevertheless, the post-mortem urea and creatinine concentrations correlate with the ante-mortem serum levels of these parameters, and they could give a valuable insight into the metabolic status before death (HANNA et al, 1990;ZHU et al, 2007).…”

Section: Discussionmentioning

confidence: 57%

Estimation of red deer (Cervus elaphus) post mortal interval based on the biochemical parameters of vitreous fluid using linear regression analyses

Severin¹,

Džaja²,

Šperanda³

et al. 2018

Vet. arhiv

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ŠATROVIĆ, K. STARČEVIĆ: Estimation of red deer (Cervus elaphus) post mortal interval based on the biochemical parameters of vitreous fluid using linear regression analyses. Vet. arhiv 88, 511-519, 2018. ABSTRACT Vitreous fluid is a stable biological fluid, which is well-protected from contamination and post mortem degradation, which makes it suitable in forensic pathology for determination of the post mortem interval (PMI). The present study was conducted to discover the correlation between increasing PMI and levels of various vitreous biochemical parameters (sodium, calcium, magnesium, chloride, potassium, phosphor, AST, urea, creatinine, total protein, glucose, total cholesterol). The estimation of PMI in red deer is of great interest in cases of illegal hunting. The vitreous humour samples were collected from six red deer carcasses. The eyes were enucleated, covered by parafilm and aluminium foil, packed in a small container and maintained at +4 ºC. Sampling of vitreous fluid was done again at 8, 10 and then regularly each 10 hours until finally 90 hours after death. A significant linear correlation was found for potassium, sodium and chloride (P<0.0001). The present study revealed that biochemical analysis of vitreous fluid could be used to determine post mortem interval in red deer.

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Section: Discussionmentioning

confidence: 57%

Estimation of red deer (Cervus elaphus) post mortal interval based on the biochemical parameters of vitreous fluid using linear regression analyses

Severin¹,

Džaja²,

Šperanda³

et al. 2018

Vet. arhiv

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“…Hx concentration in the blood increases after death because of hypoxia, promoting Hx generation from adenosine monophosphate (AMP) [ 47 , 48 ]. Hypoxanthine has already been considered a potential marker of hypoxia and PMI for many years, especially in anatomically isolated fluids, such as ocular fluids: aqueous humor (AH) and vitreous humor (VH) [ 15 , 49 , 50 , 51 ]. AH and VH have advantages over blood PMI biomarkers because postmortem metabolomic changes are affected by many processes in the body.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Animal Model for Estimating the Time since Death with Attention to Early Postmortem Stage

et al. 2022

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Estimating the postmortem interval (PMI) has remained the subject of investigations in forensic medicine for many years. Every kind of death results in changes in metabolites in body tissues and fluids due to lack of oxygen, altered circulation, enzymatic reactions, cellular degradation, and cessation of anabolic production of metabolites. Metabolic changes may provide markers determining the time since death, which is challenging in current analytical and observation-based methods. The study includes metabolomics analysis of blood with the use of an animal model to determine the biochemical changes following death. LC-MS is used to fingerprint postmortem porcine blood. Metabolites, significantly changing in blood after death, are selected and identified using univariate statistics. Fifty-one significant metabolites are found to help estimate the time since death in the early postmortem stage. Hypoxanthine, lactic acid, histidine, and lysophosphatidic acids are found as the most promising markers in estimating an early postmortem stage. Selected lysophosphatidylcholines are also found as significantly increased in blood with postmortal time, but their practical utility as PMI indicators can be limited due to a relatively low increasing rate. The findings demonstrate the great potential of LC-MS-based metabolomics in determining the PMI due to sudden death and provide an experimental basis for applying this attitude in investigating various mechanisms of death. As we assume, our study is also one of the first in which the porcine animal model is used to establish PMI metabolomics biomarkers.

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Post-mortem changes in the metabolomic compositions of rabbit blood, aqueous and vitreous humors

et al. 2016

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Simultaneous analysis of biochemical markers in vitreous humour and serum: a preliminary study on the effect of storage time at –20°C

Cited by 5 publications

References 31 publications

Estimation of red deer (Cervus elaphus) post mortal interval based on the biochemical parameters of vitreous fluid using linear regression analyses

Estimation of red deer (Cervus elaphus) post mortal interval based on the biochemical parameters of vitreous fluid using linear regression analyses

In Vitro Animal Model for Estimating the Time since Death with Attention to Early Postmortem Stage

Post-mortem changes in the metabolomic compositions of rabbit blood, aqueous and vitreous humors

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