Simultaneous 2nd Order Derivative Spectrophotometry Determination of Sorbic and Benzoic Acids in Soft Drinks

Spectrophotometric determination of sorbic and benzoic acids in fruit juices by a net analyte signal-based method with selection of the wavelength range to avoid non-modelled interferences

“…On the other hand, capillary electrophoresis has been extensively used to develop methods to analyse these preservatives in food samples, the technique micellar electrokinetic capillary chromatography (MEKC) being the one most usually applied [3,8,9,10,11]. Other methods have been reported including: second-order derivative spectrophotometry after solvent extraction [12], polarography [13] and enzymatic [14]. The AOAC official methods for analysing sorbic acid in beverages involve steam distillation followed by UV absorption spectrometry at 260 nm or reaction with thiobarbituric acid and colorimetry at 532 nm [15,16].…”

An isocratic normal-phase high-performance liquid chromatographic method for the simultaneous determination of benzoyl peroxide and benzoic acid in one pharmaceutical preparation and their stability in different solvents

“…Benzoic acid [BA] Benzoic acid is carboxylic acid, it is present naturally either free or bounds benzoic acid esters and can be found in many natural products [10].Benzoic acid is extensively used as preserving agent most suitable for foods, fruit juices, and soft drinks. It is harmful at higher than permitted safety levels [11].In humans, the acute toxicity of benzoic acid is low symptoms appear shortly after exposure and disappear within a fewhours, even at low doses [12].Analytical methods for the determination of benzoic acid include spectrophotometric methods [13], which need extensive extraction procedures and are not very specific; gas chromatographic (GC) methods [14], which are more sensitive and specific but need lengthy sample preparation and derivatization prior to determination using high-performance liquid chromatography(HPLC) [15], which has a high specificity and minimum sample preparation and does not require derivatization. Other methods were used to assay benzoic acid like GC-MAS [16] and GC with on line pyrolytic methylation technique [17].…”