The aim of this study was to apply high performance liquid chromatography -UV detector to determine desmopressin in pharmaceutical preparation using one pump (isocratic pump) and ODS, 5um column. And Mobil phase acetonitrile: N, N diethyl formamide in ratio of 85:15 which show good quantitative and qualitative analysis in desmopressin acetate nasal spray (Cipla LTD INDIA) New controlled condition for setting-up the system to get a result with high resolution was shown in this study.
The mixture of paracetamol, chlorpheniramine maleate and ascorbic acid is widely used in medicine as anti flu remedy .Pharmaceutically ,this mixture isprepared as a tablet by different manufacturers and it has good reputation in the market .However, this drug mixture is still non-official in pharmacopoiea, butthe USP has described a non specific method of HPLC for the mixture of paracetamol with antihistamine and other antitussive drugs .
Application of the USP- HPLC method was found unreliable for the analysis of tablet containing this drug mixture of three components, since different chromatographic conditions should be applied for each component. Some workers also prescribed a method of analysis by specific procedure for each component individually after previous extraction, which rather long and tedious.In this work; an HPLC method was developed by using the reversed phase mode of chromatography (ODS column) and a mobile phase consists of ammonium phosphate 0.1M and methanol 20% .the peaks of three components of the studied mixture were well separated and the retention times of ascorbic acid ,chlorpheniramine maleate and paracetamol were 2.10,2.76 and 6.60 Minutes respectively and the whole analysis was accomplished in 10 minutes.The accuracy and precision of this method were approved for the quantitative analysis of each component of the mixture.
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