Simulated weightlessness and synbiotic diet effects on rat bone mechanical strength

Sarper, Hüseyin; Blanton, Cynthia; DePalma, Jude L.; Melnykov, Igor; Gabaldón, Annette M.

doi:10.1016/j.lssr.2014.09.003

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…The mechanical strength of the femur mid-diaphysis was evaluated using a three-point bending tester ( Figure 3 A). The machine was constructed by the Colorado State University-Pueblo Engineering Department and has been used in previous studies on both rat and mouse bones [ 25 , 26 ]. Prior to testing, the right femur bone from each mouse carcass was dissected from the lower extremity and all soft tissues were removed.…”

Section: Methodsmentioning

confidence: 99%

Dietary Hempseed Decreases Femur Maximum Load in a Young Female C57BL/6 Mouse Model but Does Not Influence Bone Mineral Density or Micro-Architecture

et al. 2022

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Numerous seed and seed extract diets have been investigated as a means of combating age-related bone loss, with many findings suggesting that the seeds/extracts confer positive effects on bone. Recently, there has been rising interest in the use of dietary hempseed in human and animal diets due to a perceived health benefit from the seed. Despite this, there has been a lack of research investigating the physiologic effects of dietary hempseed on bone. Previous studies have suggested that hempseed may enhance bone strength. However, a complete understanding of the effects of hempseed on bone mineralization, bone micro-architecture, and bone biomechanical properties is lacking. Using a young and developing female C57BL/6 mouse model, we aimed to fill these gaps in knowledge. From five to twenty-nine weeks of age, the mice were raised on either a control (0%), 50 g/kg (5%), or 150 g/kg (15%) hempseed diet (n = 8 per group). It was found that the diet did not influence the bone mineral density or micro-architecture of either the right femur or L5 vertebrae. Furthermore, it did not influence the stiffness, yield load, post-yield displacement, or work-to-fracture of the right femur. Interestingly, it reduced the maximum load of the right femur in the 15% hempseed group compared to the control group. This finding suggests that a hempseed-enriched diet provides no benefit to bone in young, developing C57BL/6 mice and may even reduce bone strength.

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Section: Methodsmentioning

confidence: 99%

Dietary Hempseed Decreases Femur Maximum Load in a Young Female C57BL/6 Mouse Model but Does Not Influence Bone Mineral Density or Micro-Architecture

et al. 2022

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“…1. Unscrew the crosshead beam attached to the load sensor on the three-point bending machine 7 (see 1C).…”

Section: Conversion Of a Three-point Bending Machine To A Compression...mentioning

confidence: 99%

“…However, its modification and application for use in mouse vertebral body compression testing was not been explored. The design and construction of the three-point bending machine have been previously described 7 . This report will focus on methods used to modify the machine for compression testing and to correct for system displacement.…”

Section: Introductionmentioning

confidence: 99%

Mouse Lumbar Vertebra Uniaxial Compression Testing with Embedding of the Loading Surface

Sparks,

Ansaf,

Gabaldón

2023

JoVE

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There is increasing awareness that cortical and cancellous bone differ in regulating and responding to pharmaceutical therapies, hormone therapies, and other treatments for age-related bone loss. Three-point bending is a common method used to assess the influence of a treatment on the mid-diaphysis region of long bones, which is rich in cortical bone. Uniaxial compression testing of mouse vertebrae, though capable of assessing bones rich in cancellous bone, is less commonly performed due to technical challenges. Even less commonly performed is the pairing of three-point bending and compression testing to determine how a treatment may influence a long bone's middiaphysis region and a vertebral centrum similarly or differently. Here, we describe two procedures to make compression testing of mouse lumbar vertebrae a less challenging method to perform in parallel with three-point bending: first, a procedure to convert a three-point bending machine into a compression testing machine, and second, an embedding method for preparing a mouse lumbar vertebra loading surface.

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Mimicking the effects of spaceflight on bone: Combined effects of disuse and chronic low-dose rate radiation exposure on bone mass in mice

Doherty

Genik

et al. 2017

Life Sciences in Space Research

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Simulated weightlessness and synbiotic diet effects on rat bone mechanical strength

Cited by 3 publications

References 21 publications

Dietary Hempseed Decreases Femur Maximum Load in a Young Female C57BL/6 Mouse Model but Does Not Influence Bone Mineral Density or Micro-Architecture

Dietary Hempseed Decreases Femur Maximum Load in a Young Female C57BL/6 Mouse Model but Does Not Influence Bone Mineral Density or Micro-Architecture

Mouse Lumbar Vertebra Uniaxial Compression Testing with Embedding of the Loading Surface

Mimicking the effects of spaceflight on bone: Combined effects of disuse and chronic low-dose rate radiation exposure on bone mass in mice

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