Simulação do tamanho ótimo de amostra em duas fases para um custo fixo de amostragem

Abstract

Genetic research on the coffee culture, have had great expansion and, in most cases, the samples consist of collecting leaves or fruits in different plants constituting sampling in more than one stage. In two-stage sampling or sub-sampling, designated hierarchical sampling, the population is N_1 primary units and each primary unit by N_2 individuals. n_1 primary units are selected, and each selected n_2 individuals. To determine the optimal size of the biological sample is necessary to have data obtained from experiments conducted well and faithfully express the variability between coffee plants and fruits, from plants for conditions which may vary according to the studiedgenes. In general, the size of the biological sample used may be underestimated due mainly to the relationship between the variances and cost ratio.