Simplified Tracking of a Soy Allergen in Processed Food Using a Monoclonal Antibody-Based Sandwich ELISA Targeting the Soybean 2S Albumin Gly m 8

Ueberham, Elke; Spiegel, Holger; Havenith, Heide; Rautenberger, Paul; Lidzba, Norbert; Schillberg, Stefan; Lehmann, Jörg

doi:10.1021/acs.jafc.9b02717

Cited by 27 publications

(27 citation statements)

References 47 publications

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“…Soy allergens in food samples are currently detected in most cases using ELISAs based on antibodies raised against bulk soybean proteins or specifically targeting soybean trypsin inhibitor, conglycinin, or glycinin, but the results are often incorrect because the antibodies cross-react with other proteins. Ueberham et al developed a monoclonal antibody-based sandwich ELISA targeting the soybean 2S albumin Gly m 8 soy allergen to detect traces of soy proteins in food samples ( 29 ). The various commercial ELISAs lack standardized reference material, and the results are often inaccurate because the antibodies cross-react with proteins from other legumes.…”

Section: Methods For the Detection And Analysis Of Allergensmentioning

confidence: 99%

Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

Tuzimski

Petruczynik

2020

Journal of AOAC INTERNATIONAL

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Background Allergies represent an important health problem in industrialized countries. Allergen sensitization is an important risk factor for the development of allergic diseases; thus, the identification of an individual’s allergen sensitization is essential for the diagnosis and treatment of diseases. Objective This review compares different modern methods applied for the analysis of allergens in various matrices (from 2015 to the end of September 2019). Conclusions Immunological methods are still most frequently used for detection of allergens. These methods are sensitive, but the lack of specificity and cross-reaction of some antibodies can still be a relevant source of errors. DNA-based methods are fast and reliable for determination of protein allergens, but the epitopes of protein allergens with posttranslational modifications and their changes, originated during various processing, cannot be identified through the use of this method. Methods based on application of biosensors are very rapid and easy to use, and can be readily implemented as screening methods to monitor allergens. Recent developments of new high-resolution MS instruments are encouraging and enable development in the analysis of allergens. Fast, very sensitive, reliable, and accurate detection and quantification of allergens in complex samples can be used in the near future. Mass spectrometry coupled with LC, GC, or electrophoretic methods bring additional advances in allergen analysis. The use of LC-MS or LC-MS/MS for the quantitative detection of allergens in various matrices is at present gaining acceptance as a protein-based confirmatory technique over the routinely performed enzyme-linked immunosorbent assays.

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Section: Methods For the Detection And Analysis Of Allergensmentioning

confidence: 99%

Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

Tuzimski

Petruczynik

2020

Journal of AOAC INTERNATIONAL

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“…Studies have shown that with the increase in the use of soybean and soybean products, about 1-6% of infants and children are allergic to soybean and the incidence of soybean allergies in adults is also increasing [128,129]. There are 11 kinds of allergenic proteins found in soybeans, namely: β-conglycinin (Gly m 5), hydrophobic protein, defensive protein, inhibitory protein, SAM22, 7S globulin, glycinin (Gly m 6), 2S albumin, lectin, lipoxidase, and trypsin inhibitor [130][131][132]. Among them, Gly m Bd 28K and Gly m Bd 30K of the 7S globulin and the α-subunit Gly m Bd 60K of β-conglycinin are the main allergens in soybeans.…”

Section: Soybeanmentioning

confidence: 99%

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Hong

Pan

Xie

et al. 2021

Foods

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Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.

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“…In Art v 1, the major pollen allergen from mugwort (Artemisia vulgaris) mono arabinosylated Hyp has been shown to react with serum antibodies from allergic patients [33]. While allergenic epitopes must be avoided in recombinant therapeutics, plant-based production systems offer the possibility to produce authentic recombinant allergens [34,35] to facilitate componentresolved diagnosis and subsequent immunotherapy of allergic patients.…”

Section: Glycan Engineeringmentioning

confidence: 99%

Optimizing product quality in molecular farming

Schiermeyer

2020

Current Opinion in Biotechnology

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With a first commercial biopharmaceutical product on the market, and other products proceeding through clinical development stages, the concept of plant molecular farming has proven a viable alternative to traditional production platforms. To extend the range of target molecules amenable for molecular farming, there is constant ambition to tailor the current plant host systems to improve the quality of the final product. These efforts have been focused mainly on glycan engineering, as the majority of target molecules are glycoproteins. In the past few years also more research addressed proteolytic processing of recombinant target molecules and strategies have been devised to protect valuable target proteins from degradation. Recent developments in these areas as well as in downstream processing including extraction and purification processes will be discussed.

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Simplified Tracking of a Soy Allergen in Processed Food Using a Monoclonal Antibody-Based Sandwich ELISA Targeting the Soybean 2S Albumin Gly m 8

Cited by 27 publications

References 47 publications

Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Optimizing product quality in molecular farming

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