Simplified Technique for Detection of Significant Bacteriuria by Microscopic Examination of Urine

Cardoso, Celso Luíz; Muraro, Carla Barbosa; Siqueira, Vera Lúcia Dias; Guilhermetti, Márcio

doi:10.1128/jcm.36.3.820-823.1998

Cited by 26 publications

(10 citation statements)

References 24 publications

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“…A urocultura é considerada padrão-ouro no diagnóstico de ITU (Cardoso et al, 2000;Pires et al, 2007). A infecção urinária é caracterizada pelo crescimento de, no mínimo, 100 mil unidades formadoras de colônia (UFC) por mililitro de urina colhida em jato médio e de maneira asséptica (Braoios et al, 2009;CLSI, 2009).…”

Section: Introductionunclassified

Prevalência E Resistência Bacteriana Aos Agentes Antimicrobianos De Primeira Escolha Nas Infecções Do Trato Urinário No Município De São Luís-Ma

Santana¹,

Pereira²,

Monteiro³

et al. 2012

Rev Patol Trop

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RESUMOO presente trabalho objetivou determinar a prevalência e resistência aos agentes antimicrobianos de primeira escolha dos patógenos envolvidos nas infecções do trato urinário diagnosticadas em um laboratório particular do município de São Luís-MA. Foram analisadas 875 (37%) uroculturas positivas realizadas de janeiro de 2005 a junho de 2008. Pacientes do sexo feminino foram as mais acometidas com 69% (603) dos casos. Os agentes responsáveis pelas uroculturas foram separados em três grupos e as enterobactérias predominaram entre os isolados de 85,5% das amostras (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter aerogenes e Serratia marcenses). Cocos gram-positivos (Staphylococcus aureus, Enterococus faecalis) e bacilos gram-negativos não fermentadores (Pseudomonas aeruginosa) recuperados chegaram a 89 (10,2%) e 38 (4,3%), respectivamente. Como são escassos os trabalhos de vigilância epidemiológica e laboratorial nesta região do território nacional, os dados obtidos foram relevantes no sentido de demonstrar a participação de patógenos com elevado grau de resistência aos antimicrobianos Ampicilina, Cefalotina e Sulfametoxazoltrimetropim em quadros de infecção de pacientes em tratamento ambulatorial (n=638; 73%) e pacientes hospitalizados (n=237;27%). DESCRITORES: Infecções bacterianas. Infecções por bactérias gram-negativas. Infecções por Enterobacteriaceae. Infecções por Escherichia coli.

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Section: Introductionunclassified

Prevalência E Resistência Bacteriana Aos Agentes Antimicrobianos De Primeira Escolha Nas Infecções Do Trato Urinário No Município De São Luís-Ma

Santana¹,

Pereira²,

Monteiro³

et al. 2012

Rev Patol Trop

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“…Gram stain examination was having similar sensitivity (96.0%), specificity (99.2%), positive predictive value (97.6%) and negative predictive value (98.7%) according to a study conducted by Celso Lui´Z Cardoso et al [38] from Brazil (2006). Another study conducted by Taneja et al [39] showed sensitivity, specificity, positive predictive value and negative predictive value of wet film examination for pyuria to be 68.4%, 60.8%, 32.7% and 87.3% respectively.…”

Section: Critical Care Obstetrics and Gynecology Issn 2471-9803mentioning

confidence: 64%

Microbiological Profile of Asyptomatic Bacteriuria in Pregnancy

Bose¹,

PK²

2016

Crit Care Obst & Gyne

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Background: Asymptomatic bacteriuria is the presence of actively multiplying bacteria within the urinary tract in the absence of any symptoms. Anatomical and physiological changes make women more susceptible to UTI in pregnancy.Objectives: To determine the percentage of pregnant women with asymptomatic bacteriuria and its microbiological profile.Methods: A total of 555 antenatal women who had no clinical features of urinary tract infection were recruited for this study over a period of one year. Clean catch mid-stream urine sample was collected and semi quantitatively cultured immediately. Significant bacteriuria was identified and antibiotic sensitivity found out by conventional methods.Results: Significant growth was observed in 26 samples. There was no association between age, parity, gravidity, period of gestation and asymptomatic bacteriuria. Gram stain was found to be the best screening test. Escherichia coli was the commonest organism isolated. Escherichia coli and Staphylococcus saprophyticus were the most resistant organisms. Conclusion:Escherichia coli, the most common organism isolated, was resistant to most of the commonly used antibiotics. Wet film examination, the most commonly used screening test in our set up, was less sensitive and specific. So, culture has to be done in all antenatal cases for screening asymptomatic bacteriuria of pregnancy.

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“…For physicians, this should mean prompt reporting of normal specimens and improvement in the quality of patient care. Over the past decade several rapid screening methods have been reported, including semiautomated microscopy combined with reagent strip chemical determinations (Yellow IRIS) (1), reagent strip chemical determinations alone (Clinitek 200) (2), detection of catalase activity (Uriscreen) (6), and microscopic examination of Gram-stained unspun urine (3). Results for the detection of significant bacteriuria by these methods were compared with reference urine culture results.…”

Section: Discussionmentioning

confidence: 99%

Detection of Significant Bacteriuria by Automated Urinalysis Using Flow Cytometry

et al. 2000

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A new flow cytometry-based automated urine analyzer, the UF-50, was evaluated for its ability to screen urine samples for significant bacteriuria. One hundred eighty-six urine specimens from patients attending an outpatient clinic of a university-based hospital were examined. The results obtained with the UF-50 were compared with those obtained by conventional quantitative urine culture. The UF-50 detected significant bacteriuria with a sensitivity of 83.1%, a specificity of 76.4%, a positive predictive value of 62.0%, a negative predictive value of 90.7%, and an accuracy of 78.5%. These results are comparable to those obtained by previously reported screening procedures. Besides detecting significant bacteriuria, the UF-50 can also perform routine urinalysis, including measurement of concentrations of red blood cells, white blood cells, epithelial cells, and casts, within 70 s. This capability renders this new flow cytometry-based urine analyzer superior to previously reported rapid screening methods.

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Simplified Technique for Detection of Significant Bacteriuria by Microscopic Examination of Urine

Cited by 26 publications

References 24 publications

Prevalência E Resistência Bacteriana Aos Agentes Antimicrobianos De Primeira Escolha Nas Infecções Do Trato Urinário No Município De São Luís-Ma

Prevalência E Resistência Bacteriana Aos Agentes Antimicrobianos De Primeira Escolha Nas Infecções Do Trato Urinário No Município De São Luís-Ma

Microbiological Profile of Asyptomatic Bacteriuria in Pregnancy

Detection of Significant Bacteriuria by Automated Urinalysis Using Flow Cytometry

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