Simplified rat lung transplantation using a cuff technique

Mizuta, T; Kawaguchi, Akira T.; Nakahara, Kazunari; Kawashima, Yasunaru

doi:10.1016/s0022-5223(19)34549-0

Cited by 173 publications

(68 citation statements)

References 3 publications

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“…Orthotopic left lung transplantation was performed (syngeneic: LEW to LEW, allogenic: ACI to LEW) utilizing a cuff technique as previously described (18,19). Both donor and recipient rats were mechanically ventilated with a mixture of 100% oxygen and isoflurane, positive end expiratory pressure (PEEP) of 2 cm H 2 O (tidal volume; 10 mL/kg, respiratory rate; 70/min).…”

Section: Rat Lung Transplantation (Ltx)mentioning

confidence: 99%

Carbon Monoxide Protects Rat Lung Transplants From Ischemia‐Reperfusion Injury via a Mechanism Involving p38 MAPK Pathway

Kohmoto

Nakao

Stolz

et al. 2007

American Journal of Transplantation

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Carbon monoxide (CO) provides protection against oxidative stress via anti-inflammatory and cytoprotective actions. In this study, we tested the hypothesis that a low concentration of exogenous (inhaled) CO would protect transplanted lung grafts from cold ischemia-reperfusion injury via a mechanism involving the mitogen-activated protein kinase (MAPK) signaling pathway. Lewis rats underwent orthotopic syngeneic or allogeneic left lung transplantation with 6 h of cold static preservation. Exposure of donors and recipients (1 h before and then continuously post-transplant) to 250 ppm CO resulted in significant improvement in gas exchange, reduced leukocyte sequestration, preservation of parenchymal and endothelial cell ultrastructure and reduced inflammation compared to animals exposed to air. The beneficial effects of CO were associated with p38 MAPK phosphorylation and were significantly prevented by treatment with a p38 MAPK inhibitor, suggesting that CO's efficacy is at least partially mediated by activation of p38 MAPK. Furthermore, CO markedly suppressed inflammatory events in the contralateral naïve lung. This study demonstrates that perioperative exposure of donors and recipients to CO at a low concentration can impart potent anti-inflammatory and cytoprotective effects in a clinically relevant model of lung transplantation and support further evaluation for potential clinical use.

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Section: Rat Lung Transplantation (Ltx)mentioning

confidence: 99%

Carbon Monoxide Protects Rat Lung Transplants From Ischemia‐Reperfusion Injury via a Mechanism Involving p38 MAPK Pathway

Kohmoto

Nakao

Stolz

et al. 2007

American Journal of Transplantation

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“…Lung transplantations using the cuff technique for the vascular anastomosis and sutures for the bronchial anastomosis were essentially performed as described [23]. The cuffs were made from 16 G Abbocath catheters (Abbott, Sligo, Ireland).…”

Section: Lung Transplantationmentioning

confidence: 99%

Macrophages in experimental rat lung isografts and allografts: infiltration and proliferation in situ

Schmidt

Sucke

Fuchs-Moll

et al. 2006

Journal of Leukocyte Biology

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Alveolar macrophages (AMs) and peribronchial/perivascular macrophages are probably involved in lung allograft damage. We investigate leukocyte infiltration into graft tissue and address the question whether proliferation in situ contributes to macrophage homeostasis and accumulation. Lung transplantation was performed in the Lewis (LEW)-to-LEW and in the Dark Agouti-to-LEW rat strain combination. Graft infiltration by ED1+ and ED2+ (CD163) macrophages was analyzed by immunohistochemistry (IHC) and compared with infiltration by lymphocytes. Cells in the S-phase of the cell cycle were pulse-labeled with BrdU and detected immunohistochemically. Finally, the donor or recipient origin of AMs was determined by IHC and in situ hybridization. ED1+ AMs in allogeneic transplants increased by more than 25-fold from Days 1 to 5. In addition, large, peribronchial/perivascular infiltrates developed containing numerous ED1+ cells. Although AMs in normal rat lungs are CD163-, AMs up-regulated CD163 between Days 4 and 5, reaching maximum values on Day 6. Lymphocytes were less numerous than macrophages. About 16% of the AMs and 10% of the peribronchial/perivascular macrophages were in the S-phase of the cell cycle on Day 2 post-transplantation. No differences in the frequency of BrdU+ macrophages were obvious between isografts and allografts. AMs of donor origin increased in number considerably during allograft rejection. In conclusion, the cellular infiltrate in lung allografts is dominated by macrophages, which exhibit an unusual phenotype and a strong capacity for mitotic self-renewal.

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“…First attempted in 1971 [8], then in 1982, Marck et al used microsurgery with some modifications to directly suture the hilar structures, but the technical complexity of vascular and bronchial sutures remained an obstacle [9]. Mizuta et al [10] developed their experimental model in rats using cuffs, and investigators have frequently used this model, with some modifications [11,19,20], to study ischemia-reperfusion injury in lung transplantation. However, in some reports the bronchial cuff failed to develop BOS, causing concern that the bronchial cuff was related to cuff-induced granulation tissue, which could occlude the bronchial lumen resulting in short-or long-term graft failure [12,13].…”

Section: Discussionmentioning

confidence: 99%

The orthotopic left lung transplantation in rats: a valuable experimental model without using cuff technique

Zhang¹,

Wang²,

Yin³

et al. 2008

Transplant International

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Summary Advances in the field of clinical lung transplantation must rely on observations made in animal models. In this study, we introduced a new procedure in the rat, orthotopic left lung transplantation without using the cuff technique, in which the donor pulmonary artery, pulmonary vein, and membranous parts of the bronchus were anastomosed continuously in the lumen using a mattress suture under a surgical microscope; meanwhile, a second, low‐pressure perfusion through the pulmonary artery and turnover of the vascular stump were made, which also made the vessel anastomosis easy. Transplantations were completed in 68 rats (89.5%), the mean time used for suturing the left lung hilar structure was 23.5 ± 4.6 min. All lung grafts had good life‐sustaining function because of there being no cuff‐induced granulation tissue in bronchial anastomotic stoma, and three out of 12 allografts were observed with active bronchiolitis obliterans lesions at 8 weeks after transplantation. This model is a simple, valuable experimental model for studying lung transplantation and new therapies for preventing acute or chronic rejection.

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Simplified rat lung transplantation using a cuff technique

Cited by 173 publications

References 3 publications

Carbon Monoxide Protects Rat Lung Transplants From Ischemia‐Reperfusion Injury via a Mechanism Involving p38 MAPK Pathway

Carbon Monoxide Protects Rat Lung Transplants From Ischemia‐Reperfusion Injury via a Mechanism Involving p38 MAPK Pathway

Macrophages in experimental rat lung isografts and allografts: infiltration and proliferation in situ

The orthotopic left lung transplantation in rats: a valuable experimental model without using cuff technique

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