Simplified analytical methodology for glucosinolate hydrolysis products: a miniaturized extraction technique and multivariate optimization

Fusari, Cecilia M.; Ramírez, Daniela Andrea; Camargo, Alejandra Beatríz

doi:10.1039/c8ay02442a

Cited by 15 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An exploratory analysis of the efficacy of different solvents to extract PEITC was performed; 1.00 g of the pulverized WC was suspended in 15 mL of 25 mM phosphate buffer pH 7.0 for 2 h at 37 • C to allow the hydrolysis of the glucosinolates [10]. Then, 0.15 g of surfactant (Genapol X-080, Tergitol 15-S-7), or 5 mL of n-hexane, or 5 mL of ACN/CF 10/7 mixture was added.…”

Section: Extraction Proceduresmentioning

confidence: 99%

“…PEITC extracts were diluted and analysed by high-performance liquid chromatography (HPLC-DAD). The separation was carried out by applying the operating conditions described by Fusari, Ramirez, and Camargo [10], with slight modifications. Briefly, the operating conditions were as follows: gradient elution starts at 50% mobile phase A (ultra-pure water + 0.1% formic acid) and 50% mobile phase B (methanol + 0.1% formic acid), with a phase B increment up to 80% for 20 min, remaining isocratic for 10 min, and returning to 50% of phase B the next 5 min, at a continuous flow of 0.80 mL/min.…”

Section: Peit Chromatographic Analysismentioning

confidence: 99%

“…In this case, we decided simultaneously to optimise autolysis and extraction based on the surfactant concentration, incubation temperature, and pH. The time remained constant, since it has already been reported as ideal for 2 h [10]. Considering this, we selected an experimental design of the Box-Behnken type (described in Section 2.3).…”

Section: Optimised Peitc Extractionmentioning

confidence: 99%

“…Therefore, the production and extraction of PEITC from WC discards may represent an opportunity to extract it in a stable and bioactive form taking advantage of an important by-product, generating great value. In that sense, the reported work is scarce and, even more, the existent studies are not using a sustainable approach [9][10][11]. The applied methodologies, in general, are analytical and use polluting organic solvents that are unfeasible at the industrial level.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation

2020

View full text Add to dashboard Cite

Phenylethyl isothiocyanate (PEITC) was reported as a useful antioxidant, anti-inflammatory, and chemopreventive agent. Due to technological and stability issues, it is necessary to be able to extract PEITC from its natural matrix (watercress) through sustainable and scalable methodologies. In this article, we explored, for the first time, the extractive capacity of aqueous micellar systems (AMSs) of two non-ionic surfactants. For this, we compared the AMSs with conventional organic solvents. Furthermore, we developed and optimised a new integral PEITC production and extraction process by a multifactorial experimental design. Finally, we analysed the antioxidant capacity by the oxygen radical absorbance capacity (ORAC) and ABTS methods. As results, the AMSs were able to extract PEITC at the same level as the tested conventional solvents. In addition, we optimised by response surface methodology the integrated process (2.0% m/m, 25.0 °C, pH 9.0), which was equally effective (ca. 2900 µg PEITC/g watercress), regardless of the surfactant used. The optimal extracts showed greater antioxidant capacity than pure PEITC, due to other antioxidant compounds extracted in the process. In conclusion, by the present work, we developed an innovative cost-effective and low environmental impact process for obtaining PEITC extracts from watercress by-products.

show abstract

Section: Extraction Proceduresmentioning

confidence: 99%

Section: Peit Chromatographic Analysismentioning

confidence: 99%

Section: Optimised Peitc Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation

2020

View full text Add to dashboard Cite

show abstract

“…Broccoli and cauliflower have higher content of I3C, I3A, and IAA, compared with Chinese cabbage and cabbage. This result is similar to previous work, where it was found that the indole content in reproductive tissues (flower and seed) were higher than in leaf tissue . The indole DIM was not detected (below the LOD of the method) in cauliflower and Chinese cabbage.…”

Section: Resultsmentioning

confidence: 99%

Rapid measurement of indole levels in Brassica vegetables using one millilitre binary organic extraction solvent and capillary electrophoresis‐UV analysis

Suparman

Inpota

Phonchai

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Brassica vegetables contain high levels of indole compounds which have been found to provide health benefits, especially as cancer‐preventive agents. An efficient and rapid method using solvent extraction with capillary electrophoresis (CE) and ultraviolet (UV) detection was developed for the determination of four major indoles from four types of Brassica vegetables. Materials and Methods Freeze‐dried samples of four Brassica vegetables, i.e. broccoli, cauliflower, Chinese cabbage and cabbage, were selected. Hence, 1 mL of the binary solvent dimethylformamide (DMF)–methanol, 4:1 (v/v), was used for sample extraction. The extracts were diluted with the running buffer and directly analysed using CE with UV detection of four indole compounds. Results The binary solvent DMF–methanol, 4:1 (v/v) was selected from studies of the extraction efficiency of standard indoles spiked in ivy gourd (as the negative control sample) and using diphenylamine as the internal standard. Recovery was 80(±10)–120(±3)% for the four indoles: indole‐3‐carbinol (I3C), indole‐3‐acetonitrile (I3A), indole‐3‐acetic acid (IAA), and 3,3′‐diindolylmethane (DIM). For direct analysis suitable dilution of the extract with the running buffer was required. The linear range of the quantitation is 0.75–25.0 μg/mL, limit of detection (LOD) of 0.14–0.52 μg/mL and r2 > 0.998. The amount of indole in the Brassica vegetables are in the order I3C > > IAA, I3A > DIM. Conclusion A rapid method for extraction and quantitation of four indoles in four Brassica vegetables using CE with UV detection was developed. It has the potential as an efficient technique for generating data for use in agricultural and nutritional studies.

show abstract

Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation

Ramírez

Beretta

Torres‐Palazzolo

et al. 2023

Food Safety and Health

Self Cite

View full text Add to dashboard Cite

Radishes (Raphanus sativus L.) are cruciferous vegetables with remarkable nutraceutical properties given their distinctive isothiocyanates (ITCs) profile. These compounds are formed after glucosinolates‐Myrosinase enzymolysis. Although it is important to characterize radishes' ITCs levels, it is also necessary to evaluate the bioactive compounds' physiological fate after radishes ingestion. To do so, the extraction techniques should adapt to such conditions of high aqueous environment. In this work, we studied the bioaccessibility of ITCs and indole‐3‐carbinol (I3C) in radishes taproots considering the analytical implications of this biological process. Results showed that ITCs and indole profiles in the radish's taproots after aqueous‐myrosinase hydrolysis followed by Dispersive Liquid‐Liquid Microextraction were distinctively different from other reports. After in vitro digestion, raphasatin showed the highest bioaccessibility despite its low quantitative yields. Notably, I3C and S‐Sulforaphene become promising phytochemicals, due to their bioaccessibility and their considerable remaining amounts after digestion.

show abstract

Simplified analytical methodology for glucosinolate hydrolysis products: a miniaturized extraction technique and multivariate optimization

Abstract: Miniaturized extraction techniques are one of the most significant advances in analytical chemistry today.

Cited by 15 publications

References 39 publications

Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation

Phenylethyl Isothiocyanate Extracted from Watercress By-Products with Aqueous Micellar Systems: Development and Optimisation

Rapid measurement of indole levels in Brassica vegetables using one millilitre binary organic extraction solvent and capillary electrophoresis‐UV analysis

Raphanus sativus functional potential: Impact of the analytical extraction technique on the bioaccessibility interpretation

Contact Info

Product

Resources

About