Simple Sequence Repeats in the National Longitudinal Study of Adolescent Health: An Ethnically Diverse Resource for Genetic Analysis of Health and Behavior

Haberstick, Brett C.; Smolen, Andrew; Stetler, Gary L.; Tabor, Joyce W.; Roy, Taylor; H., Rick Casey,; Pardo, A. Meijide; Roy, Forest; A., Ryals, Lauren; Hewitt, Christina; Whitsel, Eric A.; Halpern, Carolyn Tucker; Killeya‐Jones, Ley A.; Lessem, Jeffrey M.; Hewitt, John K.; Harris, Kathleen Mullan

doi:10.1007/s10519-014-9662-x

Cited by 17 publications

(25 citation statements)

References 45 publications

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“…These studies included the: 1) National Longitudinal Study of Adolescent to Adult Health (Add Health; n = 14,784; Harris et al, 2013; Haberstick et al, 2014); 2) Genes in Context (GIC; n = 1,368; Foshee et al, 2014); 3) Rochester Youth and Development Study (RYDS; n = 784; Thornberry et al, 2013); 4) Singapore Cardiovascular Cohort Study (SCCS II; n = 4222; Kaur and Bishop, 2013); 5) Family Transitions Project (FTP; n = 2379; Conger et al, 2012); 6) Cameroon Language and Genetics Study (CLGS; n = 529). Further information on each of these studies is provided in the online Supplement.…”

Section: Methodsmentioning

confidence: 99%

“…5HTTLPR genotype was determined using polymerase chain reaction [PCR] (Haberstick et al, 2014) using DNA collected using the Oragene system (DNAgenotek, Kanata, Ontario, Canada). PCR reactions contained one µl of DNA [20 ng or less], 1× Buffer II [Life Technologies (Life Tech), Grand Island, NY], 1.8 mM MgCl 2 , 180 µM each deoxynucleotide [dNTP, NEB], with 7’-deaza-2’-deoxyGTP (deaza-GTP, Roche Applied Science, Indianapolis, Indiana) substituted for one-half of the dGTP, 10% dimethylsulfoxide (Sigma-Aldrich, St. Louis, MO) forward (fluorescently labeled) and reverse primers, and one unit of AmpliTag Gold ® polymerase (Life Tech) in a total volume of 20 µl.…”

Section: Methodsmentioning

confidence: 99%

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Population Frequencies of the Triallelic 5HTTLPR in Six Ethnicially Diverse Samples from North America, Southeast Asia, and Africa

et al. 2015

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Genetic differences between populations are a potentially an important contributor to health disparities around the globe. As differences in gene frequencies influence study design, it is important to have a thorough understanding of the natural variation of the genetic variant(s) of interest. Along these lines, we characterized the variation of the 5HTTLPR and rs25531 polymorphisms in six samples from North America, Southeast Asia, and Africa (Cameroon) that differ in their racial and ethnic composition. Allele and genotype frequencies were determined for 24,066 participants. Results indicated higher frequencies of the rs25531 G-allele among Black and African populations as compared with White, Hispanic and Asian populations. Further, we observed a greater number of ‘extra-long’ (‘XL’) 5HTTLPR alleles than have previously been reported. Extra-long alleles occurred almost entirely among Asian, Black and Non-White Hispanic populations as compared with White and Native American populations where they were completely absent. Lastly, when considered jointly, we observed between sample differences in the genotype frequencies within racial and ethnic populations. Taken together, these data underscore the importance of characterizing the L-G allele to avoid misclassification of participants by genotype and for further studies of the impact XL alleles may have on the transcriptional efficiency of SLC6A4.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Population Frequencies of the Triallelic 5HTTLPR in Six Ethnicially Diverse Samples from North America, Southeast Asia, and Africa

et al. 2015

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“…The assay for 5HTTLPR and the SNP rs25531 are detailed in Haberstick et al 56 for the Add Health sample and in Wray et al 57 for the Singapore sample. The most common S and L alleles contain 14 or 16 repeat units, respectively.…”

Section: Methodsmentioning

confidence: 99%

Population differences in associations of serotonin transporter promoter polymorphism (5HTTLPR) di- and triallelic genotypes with blood pressure and hypertension prevalence

Williams

Bishop

Haberstick

et al. 2017

American Heart Journal

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Background Based on prior research finding the 5HTTLPR L allele associated with increased cardiovascular reactivity to laboratory stressors and increased risk of myocardial infarction, we hypothesized that the 5HTTLPR L allele will be associated with increased blood pressure (BP) and increased hypertension prevalence in 2 large nationally representative samples in the United States and Singapore. Methods Logistic regression and linear models tested associations between triallelic (L’S’, based on rs25531) 5HTTLPR genotypes and hypertension severity and mean systolic and diastolic blood pressure (SBP and DBP) collected during the Wave IV survey of the National Longitudinal Study of Adolescent to Adult Health (Add Health, N = 11,815) in 2008–09 and during 2004–07 in 4196 Singaporeans. Results In US Whites, L’ allele carriers had higher SBP (0.9 mm Hg, 95% CI = 0.26–1.56) and greater odds (OR = 1.23, 95% CI = 1.10–1.38) of more severe hypertension than those with S’S’ genotypes. In African Americans, L’ carriers had lower mean SBP (−1.27 mm Hg, 95% CI = −2.53 to −0.01) and lower odds (OR = 0.78, 95% CI = 0.65–0.94) of more severe hypertension than those with the S’S’ genotype. In African Americans, those with L’L’ genotypes had lower DBP (−1.13 mm Hg, 95% CI = −2.09 to −0.16) than S’ carriers. In Native Americans, L’ carriers had lower SBP (−6.05 mm Hg, 95% CI = −9.59 to −2.51) and lower odds of hypertension (OR = 0.34, 95% CI = 0.13–0.89) than those with the S’S’ genotype. In Asian/Pacific Islanders those carrying the L’ allele had lower DBP (−1.77 mm Hg, 95% CI = −3.16 to −0.38) and lower odds of hypertension (OR = 0.68, 95% CI = 0.48–0.96) than those with S’S’. In the Singapore sample S’ carriers had higher SBP (3.02 mm Hg, 95% CI = 0.54–5.51) and DBP (1.90 mm Hg, 95% CI = 0.49–3.31) than those with the L’L’ genotype. Conclusions These findings suggest that Whites carrying the L’ allele, African Americans and Native Americans with the S’S’ genotype, and Asians carrying the S’ allele will be found to be at higher risk of developing cardiovascular disease and may benefit from preventive measures.

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“…Genomic DNA was isolated from saliva samples collected using a commercial product (Oragene™, DNAgenotek, Kanata, Ontario, Canada), and 5HTTLPR was genotyped as described in Haberstick et al, (2014). The genotypes were distributed according to Hardy-Weinberg Equilibrium (28% l/l, 47% s/l and 25% s/s).…”

Section: Methodsmentioning

confidence: 99%

Genetic variation in the serotonin transporter gene influences ERP old/new effects during recognition memory

et al. 2015

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Recognition memory is defined as the ability to recognize a previously encountered stimulus and has been associated with spatially and temporally distinct event related potentials (ERPs). Allelic variations of the serotonin transporter gene (SLC6A4) have recently been shown to impact memory performance. Common variants of the serotonin transporter-linked polymorphic region (5HTTLPR) of the SLC6A4 gene result in long (l) and short (s) allelic variants with carriers of the s allele having lowered transcriptional efficiency. Thus, the current study examines the effects polymorphisms of the SLC6A4 gene have on performance and ERP amplitudes commonly associated with recognition memory. Electroencephalogram (EEG), genetic, and behavioral data were collected from sixty participants as they performed an item and source memory recognition task. In both tasks, participants studied and encoded 200 words, which were then mixed with 200 new words during retrieval. Participants were monitored with EEG during the retrieval portion of each memory task. EEG electrodes were grouped into four ROIs, left anterior superior, right anterior superior, left posterior superior, and right posterior superior. ERP mean amplitudes during hits in the item and source memory task were compared to correctly recognizing new items (correct rejections). Results show that s-carriers have decreased mean hit amplitudes in both the right anterior superior ROI 1000–1500 ms post stimulus during the source memory task and the left anterior superior ROI 300–500 ms post stimulus during the item memory task. These results suggest that individual differences due to genetic variation of the serotonin transporter gene influences recognition memory.

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Simple Sequence Repeats in the National Longitudinal Study of Adolescent Health: An Ethnically Diverse Resource for Genetic Analysis of Health and Behavior

Cited by 17 publications

References 45 publications

Population Frequencies of the Triallelic 5HTTLPR in Six Ethnicially Diverse Samples from North America, Southeast Asia, and Africa

Population Frequencies of the Triallelic 5HTTLPR in Six Ethnicially Diverse Samples from North America, Southeast Asia, and Africa

Population differences in associations of serotonin transporter promoter polymorphism (5HTTLPR) di- and triallelic genotypes with blood pressure and hypertension prevalence

Genetic variation in the serotonin transporter gene influences ERP old/new effects during recognition memory

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