Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Kuswandi, Bambang; Sari, Prawita; Hidayat, Moch. Amrun

doi:10.12991/jrp.2018.87

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…Since the dual indicator label could be read by the naked eye, the colour changes of the label were taken by scanometric method ( 28 , 29 ) using a flatbed scanner (Canoscan, LIDE 110, Tokyo, Japan), with the colour image resolution set at 300 dots-per-inch (dpi). Then the ImageJ® program for Windows® ( 30 ) was employed to analyze the colour value.…”

Section: Methodsmentioning

confidence: 99%

Colorimetric Paper-Based Dual Indicator Label for Real-Time Monitoring of Fish Freshness

Kuswandi

Hasanah

Pratoko

et al. 2022

Food Technol. Biotechnol. (Online)

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Research background. Fish freshness and quality monitoring are of high importance for consumers, retailers, and companies. Therefore, developing novel approaches that are simple, fast, non-destructive, and inexpensive to monitor fish freshness in real-time is of great value. One alternative is using Intelligent or smart packaging to monitor the freshness level or condition of packaged fish. Experimental approach. On-package dual indicator label based on paper-based pH sensors was developed for real-time monitoring of the milkfish (Chanos chanos) freshness. The paper-based pH sensor was prepared using bromocresol purple (BCP) and bromothymol blue (BTB) that were immobilized onto filter paper via dip-coating. Herein, the fish degradation could be monitored visually by the dual indicator label, where the BCP change from yellow to pink, then finally to purple, while the BTP change from orange to green-yellow, and finally to green-blue for fresh, medium, and spoilage respectively. Results and conclusion. The label responds sensitively to the fish freshness, in terms of its pH change due to the fish degradation as presented by the dual indicator color change to indicate the fish freshness status at room and chiller conditions. This pH change was followed by changes in the other parameters related to freshness, such as TVBN (total volatile basic amine), TVC (total viable count), texture, and odor evaluation. The threshold of fish spoilage indication was observed at 8 h in room temperature and 7 days in chiller condition as the deterioration time point was met with color changes. Thus, it can be concluded that the dual indicator label can be applied as a simple and low-cost on-package active label for fish freshness monitoring. Novelty and scientific contribution. Increasing consumer concerns for quality and safe food worldwide has boosted the search for a novel approach to food monitoring. In this work, a simple and practical on-package dual indicators label for real-time monitoring of fish freshness was developed. The colorimetric pH sensor was fabricated simply via dip-coating of filter paper, and yet easy detection using a naked eye for accurate fish spoilage. The dual indicators label changes color in a similar tendency toward other freshness parameters, such as TVBN, TVC, texture, and odor.

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Section: Methodsmentioning

confidence: 99%

Colorimetric Paper-Based Dual Indicator Label for Real-Time Monitoring of Fish Freshness

Kuswandi

Hasanah

Pratoko

et al. 2022

Food Technol. Biotechnol. (Online)

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show abstract

“…Penambahan larutan analit pada sensor akan mengubah warna sensor yang semula putih menjadi biru. Perubahan warna sensor ini ditangkap dengan pemindai dokumen (flatbed scanner) dan dikuantifikasi dengan bantuan program pengukur piksel (pixel) warna seperti ImageJ yang dikenal sebagai metode skanometri [18]- [20] . Metode skanometri sebagai bagian dari metode kolorimetri lebih ekonomis daripada metode kolorimetri umum yang menggunakan instrumen spektrofotometer atau spektrofluoremeter.…”

Section: Pemerintah Melalui Badan Pengawasan Obat Dan Makanan (Pom) D...unclassified

Pengembangan Sensor Kimia Berbasis Kertas Untuk Penetapan Kadar Kafein Sampel Kopi

Hidayat

Wahyuning²,

Ningsih³

et al. 2023

J.Ris.Kim.

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The caffeine chemical sensor was developed by co-immobilizing sodium periodate (NaIO4), 3-methyl-2-benzothiazolinone hydrazone (MBTH), and acetic acid (CH3COOH) onto paper by using an adsorption technique. The addition of caffeine solution could change the color of the sensor from white to pale blue which can be then captured by using a flatbed scanner and quantified by the ImageJ program, known as a scanometric technique. Method validation such as linearity, LOD, LOQ, precision, and accuracy of the sensor was done by using caffeine standards. The result of caffeine analysis using the developed chemical sensor-scanometric method agreed with that of the spectrophotometric method, suggesting that the developed sensor with scanometric technique can be used as an alternative method for caffeine assay in coffee samples.

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The CUPRAC-paper microzone plates as a simple and rapid method for total antioxidant capacity determination of plant extract

Hidayat

Chassana

Ningsih

et al. 2019

Eur Food Res Technol

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Simple scanometric assay based on DPPH immobilized on pharmaceutical blister for determination of antioxidant capacity in the herbal extracts

Cited by 3 publications

References 43 publications

Colorimetric Paper-Based Dual Indicator Label for Real-Time Monitoring of Fish Freshness

Colorimetric Paper-Based Dual Indicator Label for Real-Time Monitoring of Fish Freshness

Pengembangan Sensor Kimia Berbasis Kertas Untuk Penetapan Kadar Kafein Sampel Kopi

The CUPRAC-paper microzone plates as a simple and rapid method for total antioxidant capacity determination of plant extract

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