Simple, rapid, and sensitive on-site detection of Hg2+ in water samples through combining portable evanescent wave optofluidic biosensor and fluorescence resonance energy transfer principle

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

“…Optical fibers can be chemically modified at the interface before use or left unmodified, depending on the different sensing mechanisms. Unmodified optical fibers have been used for the detection of diverse types of targets including bacteria and metal ions [ 60 ]. Taking advantage of the large size of bacteria (at least submicron in diameter) and the short evanescent wave depth (typically less than 100 nm), an unmodified bare SiO 2 fiber has been used for the detection of E. coli O157:H7.…”

“…Many works use a similar approach to immobilize amino aptamers on the fiber surface with slight differences [ 51 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. For examples, glycine [ 60 , 79 ] and NaCNBH 3 [ 60 , 80 ] were used to deplete the remaining aldehyde sites after the coupling of aptamers. A similar route has also been used for the attachment of nucleic acid probes on PMMA fibers [ 81 ].…”

A novel sensitive DNAzyme-based optical fiber evanescent wave biosensor for rapid detection of Pb2+ in human serum

“…According to the open reports, pH would affect the DNA hybridization, the activity of GR-5E-BHQ2 or the structure and morphology of Pb 2+ . 21 To obtain the optimal pH condition, MOP solution with different pH values including 2, 3, 4, 5, 6, 7, 8, 9, and 11 was applied to prepare GR-5S-Cy3, GR-5E-BHQ2 and Pb 2+ solution with the optimal concentrations. After the pre-reaction, the mixture samples were pumped into the optofluidic cell for detection.…”

Screen‐printed electrode‐based homogeneous electrochemical aptasensor for mercury (II) based on reduced graphene oxide and exonuclease III‐driven cyclic reaction

“…At present, the main detection methods of Hg 2+ include fluorescence spectrometry, [3][4][5] atomic absorption/emission spectrometry, [6][7][8] inductively coupled plasma mass spectrometry, [9][10][11] surface-enhanced Raman scattering [12][13][14] and electrochemical sensors [15,16] and so on. Among these methods, electrochemical methods have attracted much attention due to their advantages of low cost, fast response, easy miniaturization of the instrument, and being suitable for field detection.…”