A novel sensitive DNAzyme-based optical fiber evanescent wave biosensor for rapid detection of Pb²⁺ in human serum

Abstract

We described here a portable DNAzyme-based optical fiber evanescent wave biosensor (OFEWB) for rapid and sensitive detection of Pb2+ in human serum. Differing from other biosensors, the complex biorecognition element...

Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors

“…The DNAzyme 8–17 and GR-5 were respectively used to construct the separation-free FOEW sensors by direct detection of the fluorophore-labeled cleaved substrate (LOD = 20 nM) either using the cDNA functionalized fiber ( Figure 13 C) [ 79 ] or the bare fiber ( Figure 13 D) [ 100 ]. The hybridization and the nonspecific absorption were respectively utilized to capture the cleaved substrate.…”

“… Schematic diagrams of FOEW sensors for Pb 2+ detection using ( A ) G-rich probe [ 99 ] or DNAzymes via ( B ) offline magnetic-separation-based strategy [ 65 ] and ( C , D ) separation-free means enabled by cDNA functionalized fiber [ 79 ] or bare fiber [ 100 ]. …”

Fast and sensitive graphene oxide‐DNAzyme‐based biosensor for Vibrio alginolyticus detection

“…Moreover, its preparation and storage costs are very low and they are easy to chemically synthesize, modify, and combine with other methods (Huo et al., 2020). These advantages have promoted its development and application in the fields of biosensing, tumor therapy, genetic diagnosis, and bioimaging (Li et al., 2018; Wang et al., 2017a). In recent years, there have been many reports on the application of DNAzyme in pathogen detection.…”

Liquid-Core Hydrogel Optical Fiber Fluorescence Probes

“…Optical fiber fluorescence probes, which detect targets based on fluorescence signal sensing and quantification, have the advantages of compact configuration, signal transmission-detection integration, and remote detection capability. − Therefore, optical fiber fluorescence probes are widely employed in various chemical, biological, and medical , applications, especially those facing outdoor detection challenges. Many optical fiber fluorescence probes have been prepared based on commercial silica optical fibers. , One classical preparation tactic is modifying sensitive materials on the fiber side surfaces. In detail, after fiber cladding corrosion, sensitive materials can be modified on the bare core to bind targets, thus inducing changes in fluorescence signals for target sensing. , Unfortunately, these optical fiber fluorescence probes suffer from a series of shortcomings.…”

“…Many optical fiber fluorescence probes have been prepared based on commercial silica optical fibers. 9,10 One classical preparation tactic is modifying sensitive materials on the fiber side surfaces. In detail, after fiber cladding corrosion, sensitive materials can be modified on the bare core to bind targets, thus inducing changes in fluorescence signals for target sensing.…”