Simple, rapid and sensitive method for the determination of indomethacin in plasma by high-performance liquid chromatography with ultraviolet detection

Sato, Juichi; Amizuka, Takasuke; Niida, Yuichi; Umetsu, Masao; Ito, Keiji

doi:10.1016/s0378-4347(96)00463-x

Cited by 34 publications

(19 citation statements)

References 5 publications

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“…Plasma was separated by centrifugation and frozen until the measurement of indomethacin. The PIC was determined by high-performance liquid chromatography with ultraviolet detection [19] .…”

Section: Measurementsmentioning

confidence: 99%

Delayed Neonatal Closure of the Ductus Arteriosus Following Early in utero Exposure to Indomethacin in the Rat

Momma

Toyoshima²,

Ito³

et al. 2009

Neonatology

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Background: Indomethacin is used to close the patent ductus arteriosus in premature infants and for tocolysis of preterm labor. Clinically and experimentally, early in utero exposure to indomethacin induces the paradoxical delay of postnatal closure of the ductus arteriosus. Objectives: To clarify the pharmacological nature of the delay of closure of the ductus arteriosus in the rat. Methods: We studied early in utero exposure to indomethacin (dose and timing) in addition to other drugs, inducing a delay in postnatal ductal closure. Pregnant rats at near term were studied by cesarean section on gestational day 21 (D21), incubated in room air at 33°C, followed by rapid whole-body freezing. Results: The delay in closure of the ductus arteriosus was dose dependent. A large dose of indomethacin (10 mg/kg) 1 or 2 days before birth induced a delay of 3–4 times. A timing study revealed maximum delay with administration of indomethacin 2 days before birth and minimum delay with administration 5 days before. Aspirin, ibuprofen, the selective COX1 inhibitor SC 560, the selective COX2 inhibitor rofecoxib and a prostaglandin EP₄ receptor blocker, ONO-208, all delayed neonatal ductal closure following maternal administration on D19 and D20. Conclusions: The delay by indomethacin was dose dependent. The maximum delay was induced by 2 doses of 10 mg/kg indomethacin on D19 and D20. The delay was induced by a decreased stimulus to the prostaglandin EP₄ receptor system in the last 2 days in utero. The delay was temporary with recovery 3 days or more after exposure.

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“…Plasma was separated by centrifugation and frozen until the measurement of indomethacin. The PIC was determined by high-performance liquid chromatography with ultraviolet detection [19] .…”

Section: Measurementsmentioning

confidence: 99%

Delayed Neonatal Closure of the Ductus Arteriosus Following Early in utero Exposure to Indomethacin in the Rat

Momma

Toyoshima²,

Ito³

et al. 2009

Neonatology

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“…The test was performed at 37-C in an incubator-shaker at 50 rpm. Previously published method with some modifications was adopted to determine indomethacin levels in plasma (26). To a 100 ml aliquot of plasma, 100 ml of internal standard solution (mefenamic acid in acetonitrile) and 400 ml of acetonitrile were added.…”

Section: In Vitro Drug Release Studymentioning

confidence: 99%

Local Delivery of Indomethacin to Arthritis-Bearing Rats through Polymeric Micelles Based on Amphiphilic Polyphosphazenes

Zhang

Yan

et al. 2007

Pharm Res

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“…31 To a 100 lL aliquot of plasma, 100 lL of internal standard solution (mefenamic acid in acetonitrile) and 400 lL of acetonitrile were added. Samples were mixed by vortexing for 60 s and centrifuged at 8000 rpm for 5 min.…”

Section: Determination Of Ind Content In Polymeric Micelles and Rat Pmentioning

confidence: 99%

Physicochemical characterization, in vitro, and in vivo evaluation of indomethacin‐loaded nanocarriers self‐assembled by amphiphilic polyphosphazene

Zhang

et al. 2007

J Biomedical Materials Res

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Copolymeric nanocarriers assembled by amphiphilic polyphosphazene bearing poly(N-isopropylacrylamide) (PNIPAAm) and ethyl glycinate (EtGly) as substitutes, were investigated as drug vehicles for indomethacin (IND). The physicochemical characteristics of the novel nanocontainers were studied, including lower critical solution temperature (LCST), critical micelle concentration (CMC) and drug loading capacity. LCST measurements revealed that copolymer is more sensitive to the introduction of salts into aqueous solution compared with homopolymer. A significant decrease in CMC was observed when the temperature increased above LCST. As evidenced by transmission electron microscopy (TEM) measurement, morphological transformation from multicompartment into spherical nanoparticles was observed when nanocarriers with higher IND content were concerned. In vitro release tests suggested that IND-loaded nanocontainers exhibited pH dependent release profiles. In vivo pharmacokinetic study after subcutaneous administration provided a relatively sustained release behavior. Additionally, compared with free drug solution at the same dose, IND concentration in rat plasma showed a prolonged retention in experimental group treated with IND-loaded micelles. In vivo pharmacodynamic study based on both carrageenan-induced acute and complete Freund's adjuvant (CFA) induced adjuvant-arthritis models indicated that sustained therapeutic efficacy could be achieved through intraarticular injection of IND-loaded micelles. Most importantly, local delivery of IND can avoid the severe gastrointestinal stimulation, which is frequently associated with oral administration.

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Simple, rapid and sensitive method for the determination of indomethacin in plasma by high-performance liquid chromatography with ultraviolet detection

Cited by 34 publications

References 5 publications

Delayed Neonatal Closure of the Ductus Arteriosus Following Early in utero Exposure to Indomethacin in the Rat

Delayed Neonatal Closure of the Ductus Arteriosus Following Early in utero Exposure to Indomethacin in the Rat

Local Delivery of Indomethacin to Arthritis-Bearing Rats through Polymeric Micelles Based on Amphiphilic Polyphosphazenes

Physicochemical characterization, in vitro, and in vivo evaluation of indomethacin‐loaded nanocarriers self‐assembled by amphiphilic polyphosphazene

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