Simple quantitative transesterification of lipids 2. Applications

Stránský, Karel; Jursík, Tomáš

doi:10.1002/lipi.19960980207

Cited by 10 publications

(3 citation statements)

References 39 publications

(2 reference statements)

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“…After centrifugation, the supernatant was directly analyzed by GC or GC-MS. Using this method, fatty acid methyl esters were more easily prepared also from the free fatty acids and from free hydroxy acids [51,52].…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Non‐Polar Lipid Components of Human Cerumen

Stránský

Valterová

Kofroňová

et al. 2011

Lipids

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Human cerumen was separated by column chromatography into the following groups of compounds: hydrocarbons, squalene, wax esters and cholesterol esters, triacylglycerols, free fatty acids, free fatty alcohols, monoacylglycerols, free cholesterol, free sterols, and free hydroxy acids. The groups of compounds obtained were examined in detail by gas chromatography and gas chromatography-mass spectrometry. In total, about one thousand compounds have been identified.

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Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Non‐Polar Lipid Components of Human Cerumen

Stránský

Valterová

Kofroňová

et al. 2011

Lipids

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“…The TAG fractions were transesterified using a method described earlier [21]. Briefly, the TAG were dissolved in CHCl 3 /CH 3 OH (2:3, v/v) in a small glass ampoule.…”

Section: Transesterificationmentioning

confidence: 99%

Changes in the Composition of Triacylglycerols in the Fat Bodies of Bumblebee Males During Their Lifetime

Jiroš

Cvačka

Hanus

et al. 2011

Lipids

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The age-dependent changes in the composition of triacylglycerols (TAG) in the fat bodies of bumblebee males were studied using HPLC/MS. Two related species (Bombus terrestris and B. lucorum) were compared, with the age of the males being 0-30 days. The total amount of TAG in B. lucorum was about 2.7 times higher than that in B. terrestris for all of the ages studied. One to three-day-old males had the highest content of TAG in their fat bodies (1.6-2.3 mg/individual in B. terrestris and 3.8-4.2 mg/individual in B. lucorum). The analytical data show different patterns in both species. The qualitative composition of fatty acids in TAG was similar, but the mean relative abundance between B. terrestris and B. lucorum differed: 14:0, 7 and 14%; 16:0, 20 and 44%; 18:3, 62 and 23%; 18:1, 3 and 8%, respectively (the data is based on a GC/MS integration). A statistical evaluation of the dynamic changes in the TAG composition revealed that in B. terrestris different age classes were well separated according to their TAG composition while in B. lucorum the TAG did not change substantially during the male's life. The TAG analyses provide more precise information on the differences between the classes studied than the FA composition alone.

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“…The latter is a two-step method that includes an alkaline and an acid catalysis. (Liu, 1994;Stransky and Jursik, 1996;Ackman, 1998;Eras et al, 2001). Moreover, the vast majority of methods devoted to the solid and paste samples include an initial step to extract the oil or fat before the reaction with volatile esters (very often methyl esters) is carried out (Ngeh-Ngwainbi et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Note. Chlorotrimethylsilane – A Reagent for the Direct Analysis of Fats and Oils in Solid and Paste Samples

Eras¹,

Dolcet²,

Ferran

et al. 2004

Food sci. technol. int.

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Fatty acid pentyl esters (FAPEs) from eight solid and paste samples of various substances – olives, sunflower seeds, semiroasted hazelnuts, infant milk powder, mayonnaise sauce, sausage, muesli and cheese – were directly prepared using chlorotrimethylsilane (CTMS) and pentanol. The pentyl esters were analysed by GLC and the relative percentages were compared with those obtained applying the CTMS method to the oil extracted from the samples. Although significant differences in individual fatty acid compositions were observed in some cases, those differences were randomly distributed. Moreover, applying the minimum least square regression method the two sets of results might be considered statistically equivalent. Thus, calculated slope and intercept did not differ significantly from 1 and 0.

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Simple quantitative transesterification of lipids 2. Applications

Cited by 10 publications

References 39 publications

Non‐Polar Lipid Components of Human Cerumen

Non‐Polar Lipid Components of Human Cerumen

Changes in the Composition of Triacylglycerols in the Fat Bodies of Bumblebee Males During Their Lifetime

Note. Chlorotrimethylsilane – A Reagent for the Direct Analysis of Fats and Oils in Solid and Paste Samples

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