Simple purification of small-molecule-labelled peptides via palladium enolate formation from β-ketoamide tags

Abstract

A solid-supported palladium complex successfully captured β-ketoamide-tagged peptides as palladium enolates and released them in high yield upon acid treatment.

Chemical reagents for the enrichment of modified peptides in MS-based identification

“…The stability of Pd enolates in air and moisture promoted the research on their use as peptide purification systems. 26 β-Ketocarbonyl groups were tagged to peptides and they could be specifically captured by a solid-supported Pd complex in a peptide mixture. Then the captured peptides were released under treatment with an acid.…”

“…2C and D). 26,27 This review focuses on the current progress in the development of chemical reagents for the enrichment of modified peptides in MS-based identification.…”