Chemical reagents for the enrichment of modified peptides in MS-based identification

Huangfu, Shangwei; Yu, Xianqiang; Sun, Ziyu; Jiang, Biao; Chen, Hongli

doi:10.1039/d3cc05260e

Chem. Commun.

2024

DOI: 10.1039/d3cc05260e

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Chemical reagents for the enrichment of modified peptides in MS-based identification

Shangwei Huangfu,

Xianqiang Yu,

Ziyu Sun

et al.

Abstract: Chemical reagents with special groups as enrichable handles have empowered the ability to label and enrich modified peptides.

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A Highly Efficient Click Linker for Enrichment of Alkyne‐Tagged Proteins in Living Cells

Qin,

Duong,

et al. 2024

Helvetica Chimica Acta

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Alkyne tags have been widely used for the enrichment of labeled proteins to enable their profiling at a proteome‐wide scale by mass spectrometry. The key component in the enrichment process is an azido‐terminated cleavable linker for capturing the labeled proteins/peptides via click reaction. Herein, we report a new efficient click linker (APA biotin) featuring an acid‐cleavable acetal linkage end‐caped with a highly reactive picolyl azido head and a biotin handle for anchoring onto streptavidin‐coated supports. Using an amine‐reactive probe to profile the proteome structural changes in living S. cerevisiae cells within 5 minutes of heat shock, we demonstrated that the linker allowed identification of >9400 labeled sites, among which 457‐1656 with significantly altered reactivity upon heat shock. This study represented the first chemical labeling mass spectrometry (CL‐MS)‐based profiling of proteome structural changes in living cells in response to external stimuli. Data are available via ProteomeXchange with identifier PXD051279.

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A Highly Efficient Click Linker for Enrichment of Alkyne‐Tagged Proteins in Living Cells

Qin,

Duong,

et al. 2024

Helvetica Chimica Acta

View full text Add to dashboard Cite

show abstract

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Chemical reagents for the enrichment of modified peptides in MS-based identification

Abstract: Chemical reagents with special groups as enrichable handles have empowered the ability to label and enrich modified peptides.

Cited by 1 publication

References 55 publications

A Highly Efficient Click Linker for Enrichment of Alkyne‐Tagged Proteins in Living Cells

A Highly Efficient Click Linker for Enrichment of Alkyne‐Tagged Proteins in Living Cells

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