Simple methodology for methylmercury and inorganic mercury determinations by high-performance liquid chromatography–cold vapour atomic fluorescence spectrometry

Simple interface of high-performance liquid chromatography–atomic fluorescence spectrometry hyphenated system for speciation of mercury based on photo-induced chemical vapour generation with formic acid in mobile phase as reaction reagent

“…Thus, CVG have many advantages in real sample analysis. CVG is widely used as a liquid-sample introduction technique for atomic spectroscopy and as interface for HPLC and atomic spectroscopy such as atomic absorption spectrometry (AAS) [2][3][4], inductively coupled plasma mass spectrometry (ICP-MS) [5,6], atomic fluorescence spectrometry (AFS) [7][8][9][10][11][12][13][14] and atomic emission spectrometry (AES) [15]. Hydride or cold elemental vapour generation, as interface of HPLC and atomic spectroscopy is widely used for speciation of Hg, Sn, Se, As and other metals.…”

Extractive Spectrophotometric Determination of Trace Hg (II) in Eye Drops and Ayurvedic Medicines Using Pyridine 2-Carboxaldehyde 2-Hydroxybenzoylhydrazone

“…The assessment of environmental impact, predictions of mobility of mercury in the environment or evaluation of concentrations in removal technologies require the speciation of the element. For this purpose, inorganic and organic mercury species have been separated by GC or HPLC 32,33 and references therein. GC techniques require long clean-up procedures, and several organomercury compounds are thermally stable or strongly retained by the columns26.…”

UV Irradiation Controlled Cold Vapor Generation Using SnCl2 as Reductant for Mercury Speciation

“…Traditionally, SnCl2 is used for the determination of mercury by cold vapor atomic absorption spectrometry. Occasionally, SnCl2 has also been used as a reductant for speciation analysis of mercury, but additional pretreatments such as microwave digestion 31 or UV irradiation 32 with or without oxidation reagent before reducing MeHg to Hg 0 is necessary. It was found in this work that SnCl2 cannot reduce MeHg to Hg 0 , except under UV irradiation.…”

“…The most common methods for mercury speciation are gas chromatography (GC) and highperformance liquid chromatography (HPLC) coupled with a mercury-specific detection system including atomic absorption spectrometry (AAS) 3,4 atomic fluorescence spectrometry (AFS), [5][6][7][8][9] atomic emission spectrometry (AES), [10][11][12] or inductively coupled plasma-mass spectrometry (ICP-MS). [13][14][15] Although these detection methods are attractive for mercury speciation because of their high sensitivity and selectivity, the relatively high instrumental and/or running costs as well as relatively complicated instrumentation and operation are the drawbacks of these hyphenated techniques for routine speciation analysis of mercury species.…”