Simple Method for Determining Biovar and Serovar Types of
            <i>Ureaplasma urealyticum</i>
            Clinical Isolates Using PCR–Single-Strand Conformation Polymorphism Analysis

Pitcher, D.G.; Sillis, Margaret; Robertson, Janet A.

doi:10.1128/jcm.39.5.1840-1844.2001

Cited by 21 publications

(10 citation statements)

References 30 publications

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“…On top of that, ureaarginine broth used in Mycoplasma IST is suitable for isolation of mycoplasmal DNA (12). This is why in the case of PCR detection we only used samples positive in Mycoplasma IST2.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland

et al. 2009

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The aim of this study was to evaluate the occurrence of genital mycoplasmas, especially Ureaplasma parvum and Ureaplasma urealyticum, in women with atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL) and high grade squamous intraepithelial lesions (HSIL), compared to women with normal cytology living in Katowice, Poland. Two sterile swabs were used to obtain material from the posterior vaginal fornix of 143 women with squamous intraepithelial lesions and 39 healthy women: first for general bacteriology, second for detection of urogenital mycoplasmas using Mycoplasma IST2 kit. From each positive Mycoplasma IST2 culture DNA was isolated and PCR was performed for identification of U. parvum and U. urealyticum. Mycoplasma IST was positive in 34.1% cases. Urogenital mycoplasmas were demonstrated in women with HSIL significantly more often compared to women with LSIL, ASCUS, and with normal cytology. DNA of U. parvum was demonstrated in majority of Mycoplasma IST2-positive cases, U. urealyticum DNA-only in 9 (4.9%). Predominance of 3/14 serovars of U. parvum was demonstrated. U. urealyticum biovar 2 was present more often in women with squamous intraepithelial lesions.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland

et al. 2009

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“…the 16S-23S rRNA intergenic regions), should be considered. R The usefulness of biovar-specific PCRs for biotyping wild-type isolates has been demonstrated for serotyped isolates by using a pair of PCRs based upon 16S rRNA genes (Robertson et al, 1993) and multiple-banded antigen genes (Pitcher et al, 2001), and for nonserotyped isolates by using a number of different PCRs. These include single-test PCRs based upon the following : 16S rRNA genes (Jacobs et al, 1994 ;Abele-Horn et al, 1997) ; urease gene regions (Blanchard et al, 1993) by those workers and by Povlsen et al, 1998 ;multiple-banded antigen genes (PCRs ;Teng et al, 1995) by Nelson et al (1998) and Kong et al (2000) ; and by an arbitrarily primed PCR (Grattard et al, 1995a, b). plasmas (serovars 2, 4, 5, 7, 8, 9, 10, 11, 12 or 13) but not to any antigenic determinants associated with the proposed U. parvum (parvo biovar of human ureaplasmas or its serovars 1, 3, 6 or 14).…”

Section: Description Of Ureaplasma Parvummentioning

confidence: 99%

Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001.

Robertson¹,

Stemke²,

Davis³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

162

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The phenotypic and genotypic properties of Ureaplasma urealyticum (family Mycoplasmataceae, order Mycoplasmatales, class Mollicutes) are reviewed here. The 14 recognized serovar standard strains found in humans exhibit no serological cross-reactivity with ureaplasmas from other hosts and uniquely express human immuoglobulin A1 protease activity. However, they exhibit many characteristics which place them in two distinct clusters known as the parvo biovar (or biovar 1 or B) and the T960T biovar (or biovar 2 or A). Established phenotypic markers of the biovars include clustering of antigenic types, polypeptide patterns of whole-cell preparations, differential inhibition by manganese, and polymorphism among their ureases, pyrophosphatases and diaphorases. Established genotypic markers of the biovars are DNA-DNA hybridization of 60% between biovars, and distinctive RFLP patterns and genome sizes. Divergent nucleotide sequences of several highly conserved genes attest to the phylogenetic distinctiveness of the two biovars. PCRs founded upon the sequences for 16S rRNA, the 16S-23S rRNA intergenic regions, the genus-defining urease, the serovar-defining, multiple-banded antigen genes or randomly amplified polymorphic DNA tests differentiate the biovars unambiguously. With the availability of rapid, reliable and economical tests for biovar determination, it is now appropriate to propose that the taxonomic status of U. urealyticum be emended. Serovar standard strains exhibiting traits of biovar parvo (serovars 1, 3, 6 and 14) will be designated as a separate species, Ureaplasma parvum sp. nov., as befits its smaller genome size. The serovar 3 standard (strain 27T) will be the type strain of U. parvum and is represented by ATCC 27815T and NCTC 11736T. Serovar standard strains exhibiting traits of biovar T960T (2, 4, 5, 7, 8T, 9, 10, 11, 12 and 13) will retain the U. urealyticum designation and type strain, the serovar 8 standard (strain T960T), represented by ATCC 27618T and NCTC 10177T.

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“…Several studies have described various schemes based on the PCR assay for organism detection and determination of biovars and serotypes of Ureaplasma spp. (151,153,219,238,294). Some of these methods have been applied directly to address the question of differential pathogenicity.…”

Section: Other Infectionsmentioning

confidence: 99%

Mycoplasmas and Ureaplasmas as Neonatal Pathogens

Katz²,

2005

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SUMMARY The genital mycoplasmas represent a complex and unique group of microorganisms that have been associated with a wide array of infectious diseases in adults and infants. The lack of conclusive knowledge regarding the pathogenic potential of Mycoplasma and Ureaplasma spp. in many conditions is due to a general unfamiliarity of physicians and microbiology laboratories with their fastidious growth requirements, leading to difficulty in their detection; their high prevalence in healthy persons; the poor design of research studies attempting to base association with disease on the mere presence of the organisms in the lower urogenital tract; the failure to consider multifactorial aspects of diseases; and considering these genital mycoplasmas only as a last resort. The situation is now changing because of a greater appreciation of the genital mycoplasmas as perinatal pathogens and improvements in laboratory detection, particularly with regard to the development of powerful molecular nucleic acid amplification tests. This review summarizes the epidemiology of genital mycoplasmas as causes of neonatal infections and premature birth; evidence linking ureaplasmas with bronchopulmonary dysplasia; recent changes in the taxonomy of the genus Ureaplasma; the neonatal host response to mycoplasma and ureaplasma infections; advances in laboratory detection, including molecular methods; and therapeutic considerations for treatment of systemic diseases.

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Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single-Strand Conformation Polymorphism Analysis

Cited by 21 publications

References 30 publications

Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland

Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland

Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001.

Mycoplasmas and Ureaplasmas as Neonatal Pathogens

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