Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland

Abstract

The aim of this study was to evaluate the occurrence of genital mycoplasmas, especially Ureaplasma parvum and Ureaplasma urealyticum, in women with atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL) and high grade squamous intraepithelial lesions (HSIL), compared to women with normal cytology living in Katowice, Poland. Two sterile swabs were used to obtain material from the posterior vaginal fornix of 143 women with squamous intraepithelial lesions and 39 healthy women: first for general bacteriology, second for detection of urogenital mycoplasmas using Mycoplasma IST2 kit. From each positive Mycoplasma IST2 culture DNA was isolated and PCR was performed for identification of U. parvum and U. urealyticum. Mycoplasma IST was positive in 34.1% cases. Urogenital mycoplasmas were demonstrated in women with HSIL significantly more often compared to women with LSIL, ASCUS, and with normal cytology. DNA of U. parvum was demonstrated in majority of Mycoplasma IST2-positive cases, U. urealyticum DNA-only in 9 (4.9%). Predominance of 3/14 serovars of U. parvum was demonstrated. U. urealyticum biovar 2 was present more often in women with squamous intraepithelial lesions.

Association of human papillomavirus and bacterial vaginosis with increased risk of high-grade squamous intraepithelial cervical lesions

“…Mollicutes have been described for over 30 years as being related to urogenital disorders. Some species are the main cause of non-gonococcal and non-chlamydial urethritis, as in the case of bacterial vaginosis 31. Mollicutes may induce the release of cytokines and chemokines and may cause intense inflammation.…”

Ureaplasma urealyticum and Ureaplasma parvum in women of reproductive age

“…Zdorowska-Stefanov et al [16] found slightly lower ureaplasma prevalence (29.8% of 541 women tested), while Kechagia et al [17] found slightly higher prevalence (37.0% of 369 women tested). Molecular based studies have shown that most of ureaplasma isolates thus far considered UU were actually UP [18][19][20]. According to Kacerovský et al [21], the prevalence of UP in a cohort of healthy women who were not pregnant was 57%, which is a much higher prevalence than those of other genital mycoplasmas, viruses, Chlamydia or GBS infections.…”

Prevalence and Antimicrobial Susceptibility ofMycoplasma hominis,Ureaplasma urealyticumandUreaplasma parvumin Individuals With or Without Symptoms of Genitourinary Infections

“…MH, UU, 그리고 UP는 비임균성 요도염, 전립선염, 자궁경부염, 자궁내막염, 산후열, 불임, 조기 분만 진통, 조기 양막 파열 등과 관 련이 있다는 보고가 있으나 [1,2,[7][8][9], 건강한 성인 남녀의 비뇨생 식기에도 정상 상재균으로 존재하기 때문에 그 병원성에 대하여는 논란이 있어 왔다 [1,2,7,10]. 따라서 이들 균들은 단순히 균이 검 출되었다는 결과만으로 치료를 시작하지 않고 임상증상을 고려하 여 치료 여부를 선택하게 된다 [11].…”