Simple liquid chromatography method for the quantification of irinotecan and SN38 in sheep plasma: Application to in vivo pharmacokinetics after pulmonary artery chemoembolization using drug eluting beads

Pulmonary artery chemoembolization in a sheep model: Evaluation of performance and safety of irinotecan eluting beads (DEB‐IRI)

“…Free irinotecan and SN38 were quantified in plasma by mean of a validated liquid chromatography-fluorescence detection method. 34 The same quantification method was applied to lung samples. The selectivity of the method was validated in drug free samples of sheep lung tissue for irinotecan and SN38.…”

“…Second, drug-specific reason is that sheep express carboxylesterase in the lung tissue, as do humans [30][31][32] ; this is the key enzyme for irinotecan activation into its cytotoxic metabolite SN38. 33 Both irinotecan and SN38 can be detected and quantified in sheep plasma 34 and lung tissue.…”

Challenging core-shell stationary phases with the separation of closely related anti-cancer compounds: performance studies and application to drug quantitation in cell cultures with multi-well plate clean-up

“…Different chromatographic approaches to the analysis of camptothecin and its clinically available derivatives (irinotecan and topotecan) have been summarized [15]. Due to the notable fluorescence of these pentacyclic compounds, fluorescence detection has been widely employed [16], since the sensitivity obtained is adequate for the determination of camptothecins levels in biological fluids and tissues [17][18][19]. Some HPLC methods were developed with the aim to distinguish and quantify separately its lactone and carboxylate forms [20], a useful piece of information in clinical practice.…”

Liquid chromatography–tandem mass spectrometry method for simultaneous determination of seven commonly used anticancer drugs in human plasma

“…So a plasma concentration method is urgently needed, which could offer ease of sample preparation, high sample throughput with a small sample size requirement, and high sensitivity and selectivity. HPLC methods combined with ultraviolet detection, fluorescence detection, or mass spectrometry for quantification of cyclophosphamide, ifosfamide, irinotecan, etoposide, gemcitabine, carboplatin or pemetrexed have been developed in the last five years [14][15][16][17][18][19][20]. In addition, a colorimetric method for the determination of cyclophosphamide and ifosphamide in pure and in dosage forms was suggested [21].…”