Challenging core-shell stationary phases with the separation of closely related anti-cancer compounds: performance studies and application to drug quantitation in cell cultures with multi-well plate clean-up

González‐Ruiz, Víctor; Olives, Ana I.; Martín, M. Antonia

doi:10.1016/j.chroma.2014.08.054

Cited by 8 publications

(7 citation statements)

References 78 publications

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Section: Results and Discussionmentioning

confidence: 80%

“…Other scientific contributions highlighted the higher performances of partially porous particles on totatlly porous packed material, specifically estimating the different contributions of all the Van Deemter terms on the column efficiency. 47,48 For all these reason and because of the experimental evidence of different LRI values obtained on the totally porous stationary phase, it is possible to assert that the analytestationary phase interaction is different between partially and totally porous columns, due to both thermodynamic and kinetic factors. The partially porous 100 mm × 2.1 mm, L × i.d., 2.0 μm d p , was selected for the evaluation of the effect of the other parameters.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Proposal of a Linear Retention Index System for Improving Identification Reliability of Triacylglycerol Profiles in Lipid Samples by Liquid Chromatography Methods

et al. 2018

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The retention index system was proposed in 1958 by Kováts. It is based on the correlation between the retention time of the analytes and the ones of a series of reference standards, making retention data dependent on the chromatographic phenomenon only, viz., on the three-term interaction analyte-stationary phase-mobile phase. Particularly in GC, because the mobile phase has a negligible influence, the retention of the analytes depends almost entirely on the stationary phase and it is as independent as possible from operating conditions. This makes retention index databases usable in samples identification at both the intra- and interlaboratory levels. The scope of this research was to create a similar identification system in LC, where the identification of unknowns is still a challenge because of the unavailability of spectral databases related to the low reproducibility of mass spectra generated by means of the atmospheric pressure ionization technique normally interfaced to LC. Lipid compounds, specifically triacylglycerols, were selected as target analytes mainly due to their regular chromatographic LC profile under reversed phase conditions. The odd carbon chain number triacylglycerol series from trinonain to trinonadecanoin was chosen as the basis of the retention index scale and a database of 209 triacylglycerols was built. For this purpose an ultra high-performance LC method, able to maximize the baseline separation of triacylglycerols in different real-world samples (vegetable oils, fish, and milk samples) was developed.

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Section: Results and Discussionmentioning

confidence: 80%

Section: ■ Results and Discussionmentioning

confidence: 99%

Proposal of a Linear Retention Index System for Improving Identification Reliability of Triacylglycerol Profiles in Lipid Samples by Liquid Chromatography Methods

et al. 2018

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“…Core–shell particles are then an appealing alternative to reduce solvent consumption with regular HPLC instrumentation. The volume of acetonitrile employed for the analysis of antitumor agents derived from camptothecin was reduced by 71% (Figure ) by using 2.6 μm core–shell particles compared to 5 μm conventional fully porous silica particles. , The chromatographic behavior and the applications of the stationary phases based on core–shell particles have been the subject of many reviews. − …”

Section: Columns and Stationary Phase Modificationsmentioning

confidence: 99%

Sustainable and Eco-Friendly Alternatives for Liquid Chromatographic Analysis

Olives

González‐Ruiz

Martín

2017

ACS Sustainable Chem. Eng.

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This Perspectives article covers the most relevant current strategies aimed at reducing the environmental fingerprint caused by mobile phases in liquid chromatography. Modern stationary phases, such as columns packed with core− shell particles or stationary phases allowing high-temperature separations, are discussed. Mobile-phase additives creating secondary partitioning equilibria (micelle-forming reagents, cyclodextrins, or ionic liquids) and alternatives to acetonitrile as organic solvent are critically compared in terms of separation efficacy and wastes' toxicity. Advantages of CO 2 and superheated solvents are also considered to complete the picture of the most recent developments in the field.

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“…In search for different selectivity stationary phases, other nonpolar sorbents were introduced and in particular surfaces bearing, among other things, aromatic ligands, e.g., phenyl, naphthyl, anthryl, pyrenyl [2][3][4][5] and pentafluorophenyl [6]. These aromatic surfaces offer additional -interactions apart from their hydrophobicity [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Robust naphthyl methacrylate monolithic column for high performance liquid chromatography of a wide range of solutes

Jonnada

Rassi

2015

Journal of Chromatography A

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Challenging core-shell stationary phases with the separation of closely related anti-cancer compounds: performance studies and application to drug quantitation in cell cultures with multi-well plate clean-up

Cited by 8 publications

References 78 publications

Proposal of a Linear Retention Index System for Improving Identification Reliability of Triacylglycerol Profiles in Lipid Samples by Liquid Chromatography Methods

Proposal of a Linear Retention Index System for Improving Identification Reliability of Triacylglycerol Profiles in Lipid Samples by Liquid Chromatography Methods

Sustainable and Eco-Friendly Alternatives for Liquid Chromatographic Analysis

Robust naphthyl methacrylate monolithic column for high performance liquid chromatography of a wide range of solutes

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